Rapid and site-specific deep phosphoproteome profiling by data-independent acquisition without the need for spectral libraries

Localizing phosphorylation sites by data-independent acquisition (DIA)-based proteomics is still challenging. Here, the authors develop algorithms for phosphosite localization and stoichiometry determination, and incorporate them into single-shot DIA-phosphoproteomics workflows.

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Autores principales:	Dorte B. Bekker-Jensen, Oliver M. Bernhardt, Alexander Hogrebe, Ana Martinez-Val, Lynn Verbeke, Tejas Gandhi, Christian D. Kelstrup, Lukas Reiter, Jesper V. Olsen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/448cb8a30d124c708cc21f898c76c600
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spelling	oai:doaj.org-article:448cb8a30d124c708cc21f898c76c6002021-12-02T16:56:49ZRapid and site-specific deep phosphoproteome profiling by data-independent acquisition without the need for spectral libraries10.1038/s41467-020-14609-12041-1723https://doaj.org/article/448cb8a30d124c708cc21f898c76c6002020-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-14609-1https://doaj.org/toc/2041-1723Localizing phosphorylation sites by data-independent acquisition (DIA)-based proteomics is still challenging. Here, the authors develop algorithms for phosphosite localization and stoichiometry determination, and incorporate them into single-shot DIA-phosphoproteomics workflows.Dorte B. Bekker-JensenOliver M. BernhardtAlexander HogrebeAna Martinez-ValLynn VerbekeTejas GandhiChristian D. KelstrupLukas ReiterJesper V. OlsenNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Dorte B. Bekker-Jensen Oliver M. Bernhardt Alexander Hogrebe Ana Martinez-Val Lynn Verbeke Tejas Gandhi Christian D. Kelstrup Lukas Reiter Jesper V. Olsen Rapid and site-specific deep phosphoproteome profiling by data-independent acquisition without the need for spectral libraries
description	Localizing phosphorylation sites by data-independent acquisition (DIA)-based proteomics is still challenging. Here, the authors develop algorithms for phosphosite localization and stoichiometry determination, and incorporate them into single-shot DIA-phosphoproteomics workflows.
format	article
author	Dorte B. Bekker-Jensen Oliver M. Bernhardt Alexander Hogrebe Ana Martinez-Val Lynn Verbeke Tejas Gandhi Christian D. Kelstrup Lukas Reiter Jesper V. Olsen
author_facet	Dorte B. Bekker-Jensen Oliver M. Bernhardt Alexander Hogrebe Ana Martinez-Val Lynn Verbeke Tejas Gandhi Christian D. Kelstrup Lukas Reiter Jesper V. Olsen
author_sort	Dorte B. Bekker-Jensen
title	Rapid and site-specific deep phosphoproteome profiling by data-independent acquisition without the need for spectral libraries
title_short	Rapid and site-specific deep phosphoproteome profiling by data-independent acquisition without the need for spectral libraries
title_full	Rapid and site-specific deep phosphoproteome profiling by data-independent acquisition without the need for spectral libraries
title_fullStr	Rapid and site-specific deep phosphoproteome profiling by data-independent acquisition without the need for spectral libraries
title_full_unstemmed	Rapid and site-specific deep phosphoproteome profiling by data-independent acquisition without the need for spectral libraries
title_sort	rapid and site-specific deep phosphoproteome profiling by data-independent acquisition without the need for spectral libraries
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/448cb8a30d124c708cc21f898c76c600
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Rapid and site-specific deep phosphoproteome profiling by data-independent acquisition without the need for spectral libraries

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