Improved Bacterial 16S rRNA Gene (V4 and V4-5) and Fungal Internal Transcribed Spacer Marker Gene Primers for Microbial Community Surveys

Improved Bacterial 16S rRNA Gene (V4 and V4-5) and Fungal Internal Transcribed Spacer Marker Gene Primers for Microbial Community Surveys

ABSTRACT Designing primers for PCR-based taxonomic surveys that amplify a broad range of phylotypes in varied community samples is a difficult challenge, and the comparability of data sets amplified with varied primers requires attention. Here, we examined the performance of modified 16S rRNA gene a...

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Bibliographic Details
Main Authors: William Walters, Embriette R. Hyde, Donna Berg-Lyons, Gail Ackermann, Greg Humphrey, Alma Parada, Jack A. Gilbert, Janet K. Jansson, J. Gregory Caporaso, Jed A. Fuhrman, Amy Apprill, Rob Knight
Format: article
Language:EN
Published: American Society for Microbiology 2016
Subjects:
microbial ecology
marker genes
primers
16S
ITS
Microbiology
QR1-502
Online Access:https://doaj.org/article/45bcc9711b4a4111b1ae30133e5925a4
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https://doaj.org/article/45bcc9711b4a4111b1ae30133e5925a4

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