Methanol-essential growth of Escherichia coli

Engineering synthetic methylotrophy remains challenging. Here, the authors engineer a methanol-essential E. coli by an in silico-guided multiple knockout approach and show a laboratory evolved strain can incorporate up to 24% methanol into core metabolites during growth.

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Autores principales:	Fabian Meyer, Philipp Keller, Johannes Hartl, Olivier G. Gröninger, Patrick Kiefer, Julia A. Vorholt
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/4654240b271f4b1c99e0ce6a7b6e08fb
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spelling	oai:doaj.org-article:4654240b271f4b1c99e0ce6a7b6e08fb2021-12-02T16:56:55ZMethanol-essential growth of Escherichia coli10.1038/s41467-018-03937-y2041-1723https://doaj.org/article/4654240b271f4b1c99e0ce6a7b6e08fb2018-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-03937-yhttps://doaj.org/toc/2041-1723Engineering synthetic methylotrophy remains challenging. Here, the authors engineer a methanol-essential E. coli by an in silico-guided multiple knockout approach and show a laboratory evolved strain can incorporate up to 24% methanol into core metabolites during growth.Fabian MeyerPhilipp KellerJohannes HartlOlivier G. GröningerPatrick KieferJulia A. VorholtNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Fabian Meyer Philipp Keller Johannes Hartl Olivier G. Gröninger Patrick Kiefer Julia A. Vorholt Methanol-essential growth of Escherichia coli
description	Engineering synthetic methylotrophy remains challenging. Here, the authors engineer a methanol-essential E. coli by an in silico-guided multiple knockout approach and show a laboratory evolved strain can incorporate up to 24% methanol into core metabolites during growth.
format	article
author	Fabian Meyer Philipp Keller Johannes Hartl Olivier G. Gröninger Patrick Kiefer Julia A. Vorholt
author_facet	Fabian Meyer Philipp Keller Johannes Hartl Olivier G. Gröninger Patrick Kiefer Julia A. Vorholt
author_sort	Fabian Meyer
title	Methanol-essential growth of Escherichia coli
title_short	Methanol-essential growth of Escherichia coli
title_full	Methanol-essential growth of Escherichia coli
title_fullStr	Methanol-essential growth of Escherichia coli
title_full_unstemmed	Methanol-essential growth of Escherichia coli
title_sort	methanol-essential growth of escherichia coli
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/4654240b271f4b1c99e0ce6a7b6e08fb
work_keys_str_mv	AT fabianmeyer methanolessentialgrowthofescherichiacoli AT philippkeller methanolessentialgrowthofescherichiacoli AT johanneshartl methanolessentialgrowthofescherichiacoli AT olivierggroninger methanolessentialgrowthofescherichiacoli AT patrickkiefer methanolessentialgrowthofescherichiacoli AT juliaavorholt methanolessentialgrowthofescherichiacoli
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Methanol-essential growth of Escherichia coli

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