Muscle transcriptome analysis reveals molecular pathways and biomarkers involved in extreme ultimate pH and meat defect occurrence in chicken

Abstract The processing ability and sensory quality of chicken breast meat are highly related to its ultimate pH (pHu), which is mainly determined by the amount of glycogen in the muscle at death. To unravel the molecular mechanisms underlying glycogen and meat pHu variations and to identify predict...

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Autores principales:	Stéphane Beauclercq, Christelle Hennequet-Antier, Christophe Praud, Estelle Godet, Anne Collin, Sophie Tesseraud, Sonia Métayer-Coustard, Marie Bourin, Marco Moroldo, Frédéric Martins, Sandrine Lagarrigue, Elisabeth Le Bihan-Duval, Cécile Berri
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/473703f56c5a4140bff8f4947517826f
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spelling	oai:doaj.org-article:473703f56c5a4140bff8f4947517826f2021-12-02T12:31:53ZMuscle transcriptome analysis reveals molecular pathways and biomarkers involved in extreme ultimate pH and meat defect occurrence in chicken10.1038/s41598-017-06511-62045-2322https://doaj.org/article/473703f56c5a4140bff8f4947517826f2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06511-6https://doaj.org/toc/2045-2322Abstract The processing ability and sensory quality of chicken breast meat are highly related to its ultimate pH (pHu), which is mainly determined by the amount of glycogen in the muscle at death. To unravel the molecular mechanisms underlying glycogen and meat pHu variations and to identify predictive biomarkers of these traits, a transcriptome profiling analysis was performed using an Agilent custom chicken 8 × 60 K microarray. The breast muscle gene expression patterns were studied in two chicken lines experimentally selected for high (pHu+) and low (pHu−) pHu values of the breast meat. Across the 1,436 differentially expressed (DE) genes found between the two lines, many were involved in biological processes related to muscle development and remodelling and carbohydrate and energy metabolism. The functional analysis showed an intensive use of carbohydrate metabolism to produce energy in the pHu− line, while alternative catabolic pathways were solicited in the muscle of the pHu+ broilers, compromising their muscle development and integrity. After a validation step on a population of 278 broilers using microfluidic RT-qPCR, 20 genes were identified by partial least squares regression as good predictors of the pHu, opening new perspectives of screening broilers likely to present meat quality defects.Stéphane BeauclercqChristelle Hennequet-AntierChristophe PraudEstelle GodetAnne CollinSophie TesseraudSonia Métayer-CoustardMarie BourinMarco MoroldoFrédéric MartinsSandrine LagarrigueElisabeth Le Bihan-DuvalCécile BerriNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stéphane Beauclercq Christelle Hennequet-Antier Christophe Praud Estelle Godet Anne Collin Sophie Tesseraud Sonia Métayer-Coustard Marie Bourin Marco Moroldo Frédéric Martins Sandrine Lagarrigue Elisabeth Le Bihan-Duval Cécile Berri Muscle transcriptome analysis reveals molecular pathways and biomarkers involved in extreme ultimate pH and meat defect occurrence in chicken
description	Abstract The processing ability and sensory quality of chicken breast meat are highly related to its ultimate pH (pHu), which is mainly determined by the amount of glycogen in the muscle at death. To unravel the molecular mechanisms underlying glycogen and meat pHu variations and to identify predictive biomarkers of these traits, a transcriptome profiling analysis was performed using an Agilent custom chicken 8 × 60 K microarray. The breast muscle gene expression patterns were studied in two chicken lines experimentally selected for high (pHu+) and low (pHu−) pHu values of the breast meat. Across the 1,436 differentially expressed (DE) genes found between the two lines, many were involved in biological processes related to muscle development and remodelling and carbohydrate and energy metabolism. The functional analysis showed an intensive use of carbohydrate metabolism to produce energy in the pHu− line, while alternative catabolic pathways were solicited in the muscle of the pHu+ broilers, compromising their muscle development and integrity. After a validation step on a population of 278 broilers using microfluidic RT-qPCR, 20 genes were identified by partial least squares regression as good predictors of the pHu, opening new perspectives of screening broilers likely to present meat quality defects.
format	article
author	Stéphane Beauclercq Christelle Hennequet-Antier Christophe Praud Estelle Godet Anne Collin Sophie Tesseraud Sonia Métayer-Coustard Marie Bourin Marco Moroldo Frédéric Martins Sandrine Lagarrigue Elisabeth Le Bihan-Duval Cécile Berri
author_facet	Stéphane Beauclercq Christelle Hennequet-Antier Christophe Praud Estelle Godet Anne Collin Sophie Tesseraud Sonia Métayer-Coustard Marie Bourin Marco Moroldo Frédéric Martins Sandrine Lagarrigue Elisabeth Le Bihan-Duval Cécile Berri
author_sort	Stéphane Beauclercq
title	Muscle transcriptome analysis reveals molecular pathways and biomarkers involved in extreme ultimate pH and meat defect occurrence in chicken
title_short	Muscle transcriptome analysis reveals molecular pathways and biomarkers involved in extreme ultimate pH and meat defect occurrence in chicken
title_full	Muscle transcriptome analysis reveals molecular pathways and biomarkers involved in extreme ultimate pH and meat defect occurrence in chicken
title_fullStr	Muscle transcriptome analysis reveals molecular pathways and biomarkers involved in extreme ultimate pH and meat defect occurrence in chicken
title_full_unstemmed	Muscle transcriptome analysis reveals molecular pathways and biomarkers involved in extreme ultimate pH and meat defect occurrence in chicken
title_sort	muscle transcriptome analysis reveals molecular pathways and biomarkers involved in extreme ultimate ph and meat defect occurrence in chicken
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/473703f56c5a4140bff8f4947517826f
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Muscle transcriptome analysis reveals molecular pathways and biomarkers involved in extreme ultimate pH and meat defect occurrence in chicken

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