Mucoadhesive, Thermoreversible Hydrogel, Containing Tetracaine-Loaded Nanostructured Lipid Carriers for Topical, Intranasal Needle-Free Anesthesia

Recent advances have been reported for needle-free local anesthesia in maxillary teeth by administering a nasal spray of tetracaine (TTC) and oxymetazoline, without causing pain, fear, and stress. This work aimed to assess whether a TTC-loaded hybrid system could reduce cytotoxicity, promote sustain...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Giovana Maria Fioramonti Calixto, Bruno Vilela Muniz, Simone R. Castro, Jaiza Samara Macena de Araujo, Klinger de Souza Amorim, Lígia N. M. Ribeiro, Luiz Eduardo Nunes Ferreira, Daniele Ribeiro de Araújo, Eneida de Paula, Michelle Franz-Montan
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	nanostructured lipid carriers thermoreversible hydrogel nasal administration mucoadhesion topical anesthesia tetracaine Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/47407dbe31b5403ca40d04eb1642c9de
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:47407dbe31b5403ca40d04eb1642c9de
record_format	dspace
spelling	oai:doaj.org-article:47407dbe31b5403ca40d04eb1642c9de2021-11-25T18:40:27ZMucoadhesive, Thermoreversible Hydrogel, Containing Tetracaine-Loaded Nanostructured Lipid Carriers for Topical, Intranasal Needle-Free Anesthesia10.3390/pharmaceutics131117601999-4923https://doaj.org/article/47407dbe31b5403ca40d04eb1642c9de2021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4923/13/11/1760https://doaj.org/toc/1999-4923Recent advances have been reported for needle-free local anesthesia in maxillary teeth by administering a nasal spray of tetracaine (TTC) and oxymetazoline, without causing pain, fear, and stress. This work aimed to assess whether a TTC-loaded hybrid system could reduce cytotoxicity, promote sustained permeation, and increase the anesthetic efficacy of TTC for safe, effective, painless, and prolonged analgesia of the maxillary teeth in dental procedures. The hybrid system based on TTC (4%) encapsulated in nanostructured lipid carriers (NLC) and incorporated into a thermoreversible hydrogel of poloxamer 407 (TTC<sub>NLC-HG4%</sub>) displayed desirable rheological, mechanical, and mucoadhesive properties for topical application in the nasal cavity. Compared to control formulations, the use of TTC<sub>NLC-HG4%</sub> slowed in vitro permeation of the anesthetic across the nasal mucosa, maintained cytotoxicity against neuroblastoma cells, and provided a three-fold increase in analgesia duration, as observed using the tail-flick test in mice. The results obtained here open up perspectives for future clinical evaluation of the thermoreversible hybrid hydrogel, which contains TTC-loaded NLC, with the aim of creating an effective, topical, intranasal, needle-free anesthesia for use in dentistry.Giovana Maria Fioramonti CalixtoBruno Vilela MunizSimone R. CastroJaiza Samara Macena de AraujoKlinger de Souza AmorimLígia N. M. RibeiroLuiz Eduardo Nunes FerreiraDaniele Ribeiro de AraújoEneida de PaulaMichelle Franz-MontanMDPI AGarticlenanostructured lipid carriersthermoreversible hydrogelnasal administrationmucoadhesiontopical anesthesiatetracainePharmacy and materia medicaRS1-441ENPharmaceutics, Vol 13, Iss 1760, p 1760 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nanostructured lipid carriers thermoreversible hydrogel nasal administration mucoadhesion topical anesthesia tetracaine Pharmacy and materia medica RS1-441
spellingShingle	nanostructured lipid carriers thermoreversible hydrogel nasal administration mucoadhesion topical anesthesia tetracaine Pharmacy and materia medica RS1-441 Giovana Maria Fioramonti Calixto Bruno Vilela Muniz Simone R. Castro Jaiza Samara Macena de Araujo Klinger de Souza Amorim Lígia N. M. Ribeiro Luiz Eduardo Nunes Ferreira Daniele Ribeiro de Araújo Eneida de Paula Michelle Franz-Montan Mucoadhesive, Thermoreversible Hydrogel, Containing Tetracaine-Loaded Nanostructured Lipid Carriers for Topical, Intranasal Needle-Free Anesthesia
description	Recent advances have been reported for needle-free local anesthesia in maxillary teeth by administering a nasal spray of tetracaine (TTC) and oxymetazoline, without causing pain, fear, and stress. This work aimed to assess whether a TTC-loaded hybrid system could reduce cytotoxicity, promote sustained permeation, and increase the anesthetic efficacy of TTC for safe, effective, painless, and prolonged analgesia of the maxillary teeth in dental procedures. The hybrid system based on TTC (4%) encapsulated in nanostructured lipid carriers (NLC) and incorporated into a thermoreversible hydrogel of poloxamer 407 (TTC<sub>NLC-HG4%</sub>) displayed desirable rheological, mechanical, and mucoadhesive properties for topical application in the nasal cavity. Compared to control formulations, the use of TTC<sub>NLC-HG4%</sub> slowed in vitro permeation of the anesthetic across the nasal mucosa, maintained cytotoxicity against neuroblastoma cells, and provided a three-fold increase in analgesia duration, as observed using the tail-flick test in mice. The results obtained here open up perspectives for future clinical evaluation of the thermoreversible hybrid hydrogel, which contains TTC-loaded NLC, with the aim of creating an effective, topical, intranasal, needle-free anesthesia for use in dentistry.
format	article
author	Giovana Maria Fioramonti Calixto Bruno Vilela Muniz Simone R. Castro Jaiza Samara Macena de Araujo Klinger de Souza Amorim Lígia N. M. Ribeiro Luiz Eduardo Nunes Ferreira Daniele Ribeiro de Araújo Eneida de Paula Michelle Franz-Montan
author_facet	Giovana Maria Fioramonti Calixto Bruno Vilela Muniz Simone R. Castro Jaiza Samara Macena de Araujo Klinger de Souza Amorim Lígia N. M. Ribeiro Luiz Eduardo Nunes Ferreira Daniele Ribeiro de Araújo Eneida de Paula Michelle Franz-Montan
author_sort	Giovana Maria Fioramonti Calixto
title	Mucoadhesive, Thermoreversible Hydrogel, Containing Tetracaine-Loaded Nanostructured Lipid Carriers for Topical, Intranasal Needle-Free Anesthesia
title_short	Mucoadhesive, Thermoreversible Hydrogel, Containing Tetracaine-Loaded Nanostructured Lipid Carriers for Topical, Intranasal Needle-Free Anesthesia
title_full	Mucoadhesive, Thermoreversible Hydrogel, Containing Tetracaine-Loaded Nanostructured Lipid Carriers for Topical, Intranasal Needle-Free Anesthesia
title_fullStr	Mucoadhesive, Thermoreversible Hydrogel, Containing Tetracaine-Loaded Nanostructured Lipid Carriers for Topical, Intranasal Needle-Free Anesthesia
title_full_unstemmed	Mucoadhesive, Thermoreversible Hydrogel, Containing Tetracaine-Loaded Nanostructured Lipid Carriers for Topical, Intranasal Needle-Free Anesthesia
title_sort	mucoadhesive, thermoreversible hydrogel, containing tetracaine-loaded nanostructured lipid carriers for topical, intranasal needle-free anesthesia
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/47407dbe31b5403ca40d04eb1642c9de
work_keys_str_mv	AT giovanamariafioramonticalixto mucoadhesivethermoreversiblehydrogelcontainingtetracaineloadednanostructuredlipidcarriersfortopicalintranasalneedlefreeanesthesia AT brunovilelamuniz mucoadhesivethermoreversiblehydrogelcontainingtetracaineloadednanostructuredlipidcarriersfortopicalintranasalneedlefreeanesthesia AT simonercastro mucoadhesivethermoreversiblehydrogelcontainingtetracaineloadednanostructuredlipidcarriersfortopicalintranasalneedlefreeanesthesia AT jaizasamaramacenadearaujo mucoadhesivethermoreversiblehydrogelcontainingtetracaineloadednanostructuredlipidcarriersfortopicalintranasalneedlefreeanesthesia AT klingerdesouzaamorim mucoadhesivethermoreversiblehydrogelcontainingtetracaineloadednanostructuredlipidcarriersfortopicalintranasalneedlefreeanesthesia AT ligianmribeiro mucoadhesivethermoreversiblehydrogelcontainingtetracaineloadednanostructuredlipidcarriersfortopicalintranasalneedlefreeanesthesia AT luizeduardonunesferreira mucoadhesivethermoreversiblehydrogelcontainingtetracaineloadednanostructuredlipidcarriersfortopicalintranasalneedlefreeanesthesia AT danieleribeirodearaujo mucoadhesivethermoreversiblehydrogelcontainingtetracaineloadednanostructuredlipidcarriersfortopicalintranasalneedlefreeanesthesia AT eneidadepaula mucoadhesivethermoreversiblehydrogelcontainingtetracaineloadednanostructuredlipidcarriersfortopicalintranasalneedlefreeanesthesia AT michellefranzmontan mucoadhesivethermoreversiblehydrogelcontainingtetracaineloadednanostructuredlipidcarriersfortopicalintranasalneedlefreeanesthesia
_version_	1718410830167408640

Mucoadhesive, Thermoreversible Hydrogel, Containing Tetracaine-Loaded Nanostructured Lipid Carriers for Topical, Intranasal Needle-Free Anesthesia

Ejemplares similares