A split protease-E. coli ClpXP system quantifies protein–protein interactions in Escherichia coli cells

Wang et al. developed a fluorescence-assisted single-cell methodology (split protease-E. coli ClpXP (SPEC)) to characterise protein-protein interactions for both eukaryotic and prokaryotic species in E. coli cells. Their method can quantify these interactions with high sensitivity, easy manipulation...

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Bibliographic Details
Main Authors: Shengchen Wang, Faying Zhang, Meng Mei, Ting Wang, Yueli Yun, Shihui Yang, Guimin Zhang, Li Yi
Format: article
Language:EN
Published: Nature Portfolio 2021
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Online Access:https://doaj.org/article/475f0a3ec6e54d2ca2b968a008ba7339
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