Biofilm and persister cell fo mation variability in clinical isolates of Klebsiella pneumoniae in Colombia
Klebsiella pneumoniae is an opportunistic pathogen associated with nosocomial infections. Persister cells are a fraction of a bacterial population that can escape antibiotic treatment and are associated with antibiotic therapy failure. In this work, we analyzed persistent cells in planktonic cul...
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| Format: | article |
| Langue: | EN ES |
| Publié: |
Pontificia Universidad Javeriana
2020
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| Accès en ligne: | https://doaj.org/article/478525ea74b142d6bf6288e96fef81a8 |
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| Résumé: | Klebsiella pneumoniae is an opportunistic pathogen associated with
nosocomial infections. Persister cells are a fraction of a bacterial
population that can escape antibiotic treatment and are associated
with antibiotic therapy failure. In this work, we analyzed persistent
cells in planktonic cultures and biofilms using 10 K. pneumoniae
clinical isolates and four different antibiotic types. The isolates had
different antibiotic susceptibility profiles that did not correlate with
their capacity to form biofilms. Persister cells were found under all
conditions tested, although their population numbers varied depending
on the antibiotic used. A larger number of persister cells were
found in biofilms than in planktonic cultures. Antibiotic treatment
with trimethoprim-sulfamethoxazole resulted in the largest persister
cell sub-population compared with other antibiotics tested, while
ciprofloxacin was the antibiotic that produced fewer persister cells. These
results indicate that K. pneumoniae clinical isolates vary not only in their
susceptibility to antibiotics but also in properties relevant to diseases, such
as biofilm formation and persister cell populations. |
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