Influence Of Combinations Of Some Permeable Cryoprotectants With Chicken And Duck Egg Yolks On Freezability And Dna Integrity Of Buffalo Spermatozoa

The present study aimed to investigate the cryoprotective effect of different combinations of glycerol (G), dimethyl sulphoxide (DMSO) and dimethylformamide (DMF) with chicken egg yolk (CEY) and duck egg yolk (DEY) on freezability of buffalo spermatozoa. Semen samples were collected from 4 buffalo...

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Autores principales:	Rawash, Z.M., Amal Z. Leil, Abeer M. Anwer, El-Badry, D.A.
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2020
Materias:	buffalo cryoprotectants duck egg yolk freezing glycerol spermatozoa Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2020.98371 https://doaj.org/article/47bc27fb47cc40568800dadfd5478a1e
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spelling	oai:doaj.org-article:47bc27fb47cc40568800dadfd5478a1e2021-12-02T17:04:24ZInfluence Of Combinations Of Some Permeable Cryoprotectants With Chicken And Duck Egg Yolks On Freezability And Dna Integrity Of Buffalo Spermatozoahttps://dx.doi.org/10.21608/javs.2020.983711687-40722090-3308https://doaj.org/article/47bc27fb47cc40568800dadfd5478a1e2020-07-01T00:00:00Zhttps://javs.journals.ekb.eg/article_98371.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308The present study aimed to investigate the cryoprotective effect of different combinations of glycerol (G), dimethyl sulphoxide (DMSO) and dimethylformamide (DMF) with chicken egg yolk (CEY) and duck egg yolk (DEY) on freezability of buffalo spermatozoa. Semen samples were collected from 4 buffalo bulls and diluted with Tris-based extender supplemented with 7% (V/V) cryoprotectant including G, DMSO, DMF or a mixture of 3.5% G + 3.5% DMSO, 3.5% G + 3.5% DMF and 3.5% DMSO + 3.5% DMF. All the fore-mentioned aliquots were divided into two equal portions; one is supplemented with chicken's egg yolk (CEY) and the other with duck's egg yolk (DEY). Post-thawing sperm motility, viability and acrosomal, plasmatic membrane and DNA integrities were assessed. The results clearly indicated that adding a mixture of 3.5% G + 3.5% DMSO with DEY to Tris extender significantly improved (p<0.01) post-thawing sperm motility, viability index and percentage of spermatozoa with intact acrosomes, plasma membrane and DNA (58.33%±1.66%, 173.33±5.46, 69.00±1.58%, 65.00±1.15% and 96.91±0.13%, respectively) compared with the use of 7% G and CEY (51.50±1.44%, 146.25±6.49, 61.00±2.30, 58.00±1.15% and 91.40±0.17, respectively). In conclusion, motility and functional integrity of cryopreserved buffalo spermatozoa could be enhanced by using Tris extender containing a mixture of 3.5% glycerol + 3.5% DMSO with duck’s egg yolk.Rawash, Z.M.Amal Z. LeilAbeer M. AnwerEl-Badry, D.A.Egyptian Society for Animal Managementarticlebuffalocryoprotectantsduck egg yolkfreezingglycerolspermatozoaZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 5, Iss 3, Pp 49-56 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	buffalo cryoprotectants duck egg yolk freezing glycerol spermatozoa Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	buffalo cryoprotectants duck egg yolk freezing glycerol spermatozoa Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Rawash, Z.M. Amal Z. Leil Abeer M. Anwer El-Badry, D.A. Influence Of Combinations Of Some Permeable Cryoprotectants With Chicken And Duck Egg Yolks On Freezability And Dna Integrity Of Buffalo Spermatozoa
description	The present study aimed to investigate the cryoprotective effect of different combinations of glycerol (G), dimethyl sulphoxide (DMSO) and dimethylformamide (DMF) with chicken egg yolk (CEY) and duck egg yolk (DEY) on freezability of buffalo spermatozoa. Semen samples were collected from 4 buffalo bulls and diluted with Tris-based extender supplemented with 7% (V/V) cryoprotectant including G, DMSO, DMF or a mixture of 3.5% G + 3.5% DMSO, 3.5% G + 3.5% DMF and 3.5% DMSO + 3.5% DMF. All the fore-mentioned aliquots were divided into two equal portions; one is supplemented with chicken's egg yolk (CEY) and the other with duck's egg yolk (DEY). Post-thawing sperm motility, viability and acrosomal, plasmatic membrane and DNA integrities were assessed. The results clearly indicated that adding a mixture of 3.5% G + 3.5% DMSO with DEY to Tris extender significantly improved (p<0.01) post-thawing sperm motility, viability index and percentage of spermatozoa with intact acrosomes, plasma membrane and DNA (58.33%±1.66%, 173.33±5.46, 69.00±1.58%, 65.00±1.15% and 96.91±0.13%, respectively) compared with the use of 7% G and CEY (51.50±1.44%, 146.25±6.49, 61.00±2.30, 58.00±1.15% and 91.40±0.17, respectively). In conclusion, motility and functional integrity of cryopreserved buffalo spermatozoa could be enhanced by using Tris extender containing a mixture of 3.5% glycerol + 3.5% DMSO with duck’s egg yolk.
format	article
author	Rawash, Z.M. Amal Z. Leil Abeer M. Anwer El-Badry, D.A.
author_facet	Rawash, Z.M. Amal Z. Leil Abeer M. Anwer El-Badry, D.A.
author_sort	Rawash, Z.M.
title	Influence Of Combinations Of Some Permeable Cryoprotectants With Chicken And Duck Egg Yolks On Freezability And Dna Integrity Of Buffalo Spermatozoa
title_short	Influence Of Combinations Of Some Permeable Cryoprotectants With Chicken And Duck Egg Yolks On Freezability And Dna Integrity Of Buffalo Spermatozoa
title_full	Influence Of Combinations Of Some Permeable Cryoprotectants With Chicken And Duck Egg Yolks On Freezability And Dna Integrity Of Buffalo Spermatozoa
title_fullStr	Influence Of Combinations Of Some Permeable Cryoprotectants With Chicken And Duck Egg Yolks On Freezability And Dna Integrity Of Buffalo Spermatozoa
title_full_unstemmed	Influence Of Combinations Of Some Permeable Cryoprotectants With Chicken And Duck Egg Yolks On Freezability And Dna Integrity Of Buffalo Spermatozoa
title_sort	influence of combinations of some permeable cryoprotectants with chicken and duck egg yolks on freezability and dna integrity of buffalo spermatozoa
publisher	Egyptian Society for Animal Management
publishDate	2020
url	https://dx.doi.org/10.21608/javs.2020.98371 https://doaj.org/article/47bc27fb47cc40568800dadfd5478a1e
work_keys_str_mv	AT rawashzm influenceofcombinationsofsomepermeablecryoprotectantswithchickenandduckeggyolksonfreezabilityanddnaintegrityofbuffalospermatozoa AT amalzleil influenceofcombinationsofsomepermeablecryoprotectantswithchickenandduckeggyolksonfreezabilityanddnaintegrityofbuffalospermatozoa AT abeermanwer influenceofcombinationsofsomepermeablecryoprotectantswithchickenandduckeggyolksonfreezabilityanddnaintegrityofbuffalospermatozoa AT elbadryda influenceofcombinationsofsomepermeablecryoprotectantswithchickenandduckeggyolksonfreezabilityanddnaintegrityofbuffalospermatozoa
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Influence Of Combinations Of Some Permeable Cryoprotectants With Chicken And Duck Egg Yolks On Freezability And Dna Integrity Of Buffalo Spermatozoa

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