Structural elucidation of the exopolysaccharide produced by Curvularia lunata isolate RJ01

Abstract. Jayus J, Akroman R, Nurhayati, Nugraha AS, Piluharto B, Seviour RJ. 2021. Structural elucidation of the exopolysaccharide produced by Curvularia lunata isolate RJ01. Biodiversitas 22: 2699-2705. An exopolysaccharide (EPS) was recovered from culture filtrates of the fungus Curvularia lunata...

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Autores principales:	JAY JAYUS, ROBBY AKROMAN, NURHAYATI NURHAYATI, ARI SATIA NUGRAHA, BAMBANG PILUHARTO, ROBERT JAMES SEVIOUR
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	curvularia lunata fungal exopolysaccharide β-glucan rheological and functional properties Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/4a52ca6d69e542898af4feedfe10d5f9
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spelling	oai:doaj.org-article:4a52ca6d69e542898af4feedfe10d5f92021-11-22T12:13:35ZStructural elucidation of the exopolysaccharide produced by Curvularia lunata isolate RJ011412-033X2085-472210.13057/biodiv/d220530https://doaj.org/article/4a52ca6d69e542898af4feedfe10d5f92021-04-01T00:00:00Zhttps://smujo.id/biodiv/article/view/7935https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Jayus J, Akroman R, Nurhayati, Nugraha AS, Piluharto B, Seviour RJ. 2021. Structural elucidation of the exopolysaccharide produced by Curvularia lunata isolate RJ01. Biodiversitas 22: 2699-2705. An exopolysaccharide (EPS) was recovered from culture filtrates of the fungus Curvularia lunata isolate RJ01 prepared in Jember, Indonesia. Based on a prominent peak at 883 cm-1, FTIR analysis suggested it is a ?-D-glucan, a proposal confirmed by subsequent nuclear magnetic resonance (NMR) analysis which showed anomeric protons omit the signal at d4.37 and 4.8 ppm, and sugar proton signal in the region of 2.5 to 4.2 ppm. High-performance liquid chromatography (HPLC) analysis of the acid hydrolysates of this glucan revealed the presence of glucose (64%) and a mixture of galactose and mannose (36%). Digestion with (1-->3)-?- and (1-->6)-?-glucanase from Acremonium sp. IMI 383068 gave consistent linear products of (1-->3)-?-glycosidic linkages, since the glucan was digested by (1-->3)-?-glucanase only. The rheological behavior of aqueous EPS solution suggests it behaves as a pseudoplastic non-Newtonian fluid and thus has the potential for use as a thickening agent in foods. This study is the first report on the structural elucidation of EPS from C. lunata.JAY JAYUSROBBY AKROMANNURHAYATI NURHAYATIARI SATIA NUGRAHABAMBANG PILUHARTOROBERT JAMES SEVIOURMBI & UNS Soloarticlecurvularia lunatafungal exopolysaccharideβ-glucanrheological and functional propertiesBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 5 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	curvularia lunata fungal exopolysaccharide β-glucan rheological and functional properties Biology (General) QH301-705.5
spellingShingle	curvularia lunata fungal exopolysaccharide β-glucan rheological and functional properties Biology (General) QH301-705.5 JAY JAYUS ROBBY AKROMAN NURHAYATI NURHAYATI ARI SATIA NUGRAHA BAMBANG PILUHARTO ROBERT JAMES SEVIOUR Structural elucidation of the exopolysaccharide produced by Curvularia lunata isolate RJ01
description	Abstract. Jayus J, Akroman R, Nurhayati, Nugraha AS, Piluharto B, Seviour RJ. 2021. Structural elucidation of the exopolysaccharide produced by Curvularia lunata isolate RJ01. Biodiversitas 22: 2699-2705. An exopolysaccharide (EPS) was recovered from culture filtrates of the fungus Curvularia lunata isolate RJ01 prepared in Jember, Indonesia. Based on a prominent peak at 883 cm-1, FTIR analysis suggested it is a ?-D-glucan, a proposal confirmed by subsequent nuclear magnetic resonance (NMR) analysis which showed anomeric protons omit the signal at d4.37 and 4.8 ppm, and sugar proton signal in the region of 2.5 to 4.2 ppm. High-performance liquid chromatography (HPLC) analysis of the acid hydrolysates of this glucan revealed the presence of glucose (64%) and a mixture of galactose and mannose (36%). Digestion with (1-->3)-?- and (1-->6)-?-glucanase from Acremonium sp. IMI 383068 gave consistent linear products of (1-->3)-?-glycosidic linkages, since the glucan was digested by (1-->3)-?-glucanase only. The rheological behavior of aqueous EPS solution suggests it behaves as a pseudoplastic non-Newtonian fluid and thus has the potential for use as a thickening agent in foods. This study is the first report on the structural elucidation of EPS from C. lunata.
format	article
author	JAY JAYUS ROBBY AKROMAN NURHAYATI NURHAYATI ARI SATIA NUGRAHA BAMBANG PILUHARTO ROBERT JAMES SEVIOUR
author_facet	JAY JAYUS ROBBY AKROMAN NURHAYATI NURHAYATI ARI SATIA NUGRAHA BAMBANG PILUHARTO ROBERT JAMES SEVIOUR
author_sort	JAY JAYUS
title	Structural elucidation of the exopolysaccharide produced by Curvularia lunata isolate RJ01
title_short	Structural elucidation of the exopolysaccharide produced by Curvularia lunata isolate RJ01
title_full	Structural elucidation of the exopolysaccharide produced by Curvularia lunata isolate RJ01
title_fullStr	Structural elucidation of the exopolysaccharide produced by Curvularia lunata isolate RJ01
title_full_unstemmed	Structural elucidation of the exopolysaccharide produced by Curvularia lunata isolate RJ01
title_sort	structural elucidation of the exopolysaccharide produced by curvularia lunata isolate rj01
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/4a52ca6d69e542898af4feedfe10d5f9
work_keys_str_mv	AT jayjayus structuralelucidationoftheexopolysaccharideproducedbycurvularialunataisolaterj01 AT robbyakroman structuralelucidationoftheexopolysaccharideproducedbycurvularialunataisolaterj01 AT nurhayatinurhayati structuralelucidationoftheexopolysaccharideproducedbycurvularialunataisolaterj01 AT arisatianugraha structuralelucidationoftheexopolysaccharideproducedbycurvularialunataisolaterj01 AT bambangpiluharto structuralelucidationoftheexopolysaccharideproducedbycurvularialunataisolaterj01 AT robertjamesseviour structuralelucidationoftheexopolysaccharideproducedbycurvularialunataisolaterj01
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Structural elucidation of the exopolysaccharide produced by Curvularia lunata isolate RJ01

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