Development of an inducible mouse model of iRFP713 to track recombinase activity and tumour development in vivo

Abstract While the use of bioluminescent proteins for molecular imaging is a powerful technology to further our understanding of complex processes, fluorescent labeling with visible light fluorescent proteins such as GFP and RFP suffers from poor tissue penetration and high background autofluorescen...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Andreas K. Hock, Eric C. Cheung, Timothy J. Humpton, Tiziana Monteverde, Viola Paulus-Hock, Pearl Lee, Ewan McGhee, Alessandro Scopelliti, Daniel J. Murphy, Douglas Strathdee, Karen Blyth, Karen H. Vousden
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4c0cb0ea555f4d09bb9cec3a0e510cea
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4c0cb0ea555f4d09bb9cec3a0e510cea
record_format	dspace
spelling	oai:doaj.org-article:4c0cb0ea555f4d09bb9cec3a0e510cea2021-12-02T12:32:47ZDevelopment of an inducible mouse model of iRFP713 to track recombinase activity and tumour development in vivo10.1038/s41598-017-01741-02045-2322https://doaj.org/article/4c0cb0ea555f4d09bb9cec3a0e510cea2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01741-0https://doaj.org/toc/2045-2322Abstract While the use of bioluminescent proteins for molecular imaging is a powerful technology to further our understanding of complex processes, fluorescent labeling with visible light fluorescent proteins such as GFP and RFP suffers from poor tissue penetration and high background autofluorescence. To overcome these limitations, we generated an inducible knock-in mouse model of iRFP713. This model was used to assess Cre activity in a Rosa Cre–ER background and quantify Cre activity upon different tamoxifen treatments in several organs. We also show that iRFP can be readily detected in 3D organoid cultures, FACS analysis and in vivo tumour models. Taken together we demonstrate that iRFP713 is a progressive step in in vivo imaging and analysis that widens the optical imaging window to the near-infrared spectrum, thereby allowing deeper tissue penetration, quicker image acquisition without the need to inject substrates and a better signal to background ratio in genetically engineered mouse models (GEMMs).Andreas K. HockEric C. CheungTimothy J. HumptonTiziana MonteverdeViola Paulus-HockPearl LeeEwan McGheeAlessandro ScopellitiDaniel J. MurphyDouglas StrathdeeKaren BlythKaren H. VousdenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Andreas K. Hock Eric C. Cheung Timothy J. Humpton Tiziana Monteverde Viola Paulus-Hock Pearl Lee Ewan McGhee Alessandro Scopelliti Daniel J. Murphy Douglas Strathdee Karen Blyth Karen H. Vousden Development of an inducible mouse model of iRFP713 to track recombinase activity and tumour development in vivo
description	Abstract While the use of bioluminescent proteins for molecular imaging is a powerful technology to further our understanding of complex processes, fluorescent labeling with visible light fluorescent proteins such as GFP and RFP suffers from poor tissue penetration and high background autofluorescence. To overcome these limitations, we generated an inducible knock-in mouse model of iRFP713. This model was used to assess Cre activity in a Rosa Cre–ER background and quantify Cre activity upon different tamoxifen treatments in several organs. We also show that iRFP can be readily detected in 3D organoid cultures, FACS analysis and in vivo tumour models. Taken together we demonstrate that iRFP713 is a progressive step in in vivo imaging and analysis that widens the optical imaging window to the near-infrared spectrum, thereby allowing deeper tissue penetration, quicker image acquisition without the need to inject substrates and a better signal to background ratio in genetically engineered mouse models (GEMMs).
format	article
author	Andreas K. Hock Eric C. Cheung Timothy J. Humpton Tiziana Monteverde Viola Paulus-Hock Pearl Lee Ewan McGhee Alessandro Scopelliti Daniel J. Murphy Douglas Strathdee Karen Blyth Karen H. Vousden
author_facet	Andreas K. Hock Eric C. Cheung Timothy J. Humpton Tiziana Monteverde Viola Paulus-Hock Pearl Lee Ewan McGhee Alessandro Scopelliti Daniel J. Murphy Douglas Strathdee Karen Blyth Karen H. Vousden
author_sort	Andreas K. Hock
title	Development of an inducible mouse model of iRFP713 to track recombinase activity and tumour development in vivo
title_short	Development of an inducible mouse model of iRFP713 to track recombinase activity and tumour development in vivo
title_full	Development of an inducible mouse model of iRFP713 to track recombinase activity and tumour development in vivo
title_fullStr	Development of an inducible mouse model of iRFP713 to track recombinase activity and tumour development in vivo
title_full_unstemmed	Development of an inducible mouse model of iRFP713 to track recombinase activity and tumour development in vivo
title_sort	development of an inducible mouse model of irfp713 to track recombinase activity and tumour development in vivo
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4c0cb0ea555f4d09bb9cec3a0e510cea
work_keys_str_mv	AT andreaskhock developmentofaninduciblemousemodelofirfp713totrackrecombinaseactivityandtumourdevelopmentinvivo AT ericccheung developmentofaninduciblemousemodelofirfp713totrackrecombinaseactivityandtumourdevelopmentinvivo AT timothyjhumpton developmentofaninduciblemousemodelofirfp713totrackrecombinaseactivityandtumourdevelopmentinvivo AT tizianamonteverde developmentofaninduciblemousemodelofirfp713totrackrecombinaseactivityandtumourdevelopmentinvivo AT violapaulushock developmentofaninduciblemousemodelofirfp713totrackrecombinaseactivityandtumourdevelopmentinvivo AT pearllee developmentofaninduciblemousemodelofirfp713totrackrecombinaseactivityandtumourdevelopmentinvivo AT ewanmcghee developmentofaninduciblemousemodelofirfp713totrackrecombinaseactivityandtumourdevelopmentinvivo AT alessandroscopelliti developmentofaninduciblemousemodelofirfp713totrackrecombinaseactivityandtumourdevelopmentinvivo AT danieljmurphy developmentofaninduciblemousemodelofirfp713totrackrecombinaseactivityandtumourdevelopmentinvivo AT douglasstrathdee developmentofaninduciblemousemodelofirfp713totrackrecombinaseactivityandtumourdevelopmentinvivo AT karenblyth developmentofaninduciblemousemodelofirfp713totrackrecombinaseactivityandtumourdevelopmentinvivo AT karenhvousden developmentofaninduciblemousemodelofirfp713totrackrecombinaseactivityandtumourdevelopmentinvivo
_version_	1718393975953424384

Development of an inducible mouse model of iRFP713 to track recombinase activity and tumour development in vivo

Ejemplares similares