Purification of cross-linked RNA-protein complexes by phenol-toluol extraction

Purification of cross-linked RNA-protein complexes by phenol-toluol extraction

RNA binding proteins are important regulators of RNA function. Here the authors describe a method for isolation of RNA-protein complexes that does not rely on a specific RNA sequence or motif, and demonstrate the approach by providing the global RNA-bound proteomes of human HEK293 cells and Salmonel...

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Autores principales: Erika C. Urdaneta, Carlos H. Vieira-Vieira, Timon Hick, Hans-Herrmann Wessels, Davide Figini, Rebecca Moschall, Jan Medenbach, Uwe Ohler, Sander Granneman, Matthias Selbach, Benedikt M. Beckmann
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/4c172901827e4b4c9950ae7fba3c2b89
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https://doaj.org/article/4c172901827e4b4c9950ae7fba3c2b89

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