Purification of cross-linked RNA-protein complexes by phenol-toluol extraction

RNA binding proteins are important regulators of RNA function. Here the authors describe a method for isolation of RNA-protein complexes that does not rely on a specific RNA sequence or motif, and demonstrate the approach by providing the global RNA-bound proteomes of human HEK293 cells and Salmonel...

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Autores principales:	Erika C. Urdaneta, Carlos H. Vieira-Vieira, Timon Hick, Hans-Herrmann Wessels, Davide Figini, Rebecca Moschall, Jan Medenbach, Uwe Ohler, Sander Granneman, Matthias Selbach, Benedikt M. Beckmann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/4c172901827e4b4c9950ae7fba3c2b89
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spelling	oai:doaj.org-article:4c172901827e4b4c9950ae7fba3c2b892021-12-02T15:35:14ZPurification of cross-linked RNA-protein complexes by phenol-toluol extraction10.1038/s41467-019-08942-32041-1723https://doaj.org/article/4c172901827e4b4c9950ae7fba3c2b892019-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-08942-3https://doaj.org/toc/2041-1723RNA binding proteins are important regulators of RNA function. Here the authors describe a method for isolation of RNA-protein complexes that does not rely on a specific RNA sequence or motif, and demonstrate the approach by providing the global RNA-bound proteomes of human HEK293 cells and Salmonella Typhimurium.Erika C. UrdanetaCarlos H. Vieira-VieiraTimon HickHans-Herrmann WesselsDavide FiginiRebecca MoschallJan MedenbachUwe OhlerSander GrannemanMatthias SelbachBenedikt M. BeckmannNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-17 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Erika C. Urdaneta Carlos H. Vieira-Vieira Timon Hick Hans-Herrmann Wessels Davide Figini Rebecca Moschall Jan Medenbach Uwe Ohler Sander Granneman Matthias Selbach Benedikt M. Beckmann Purification of cross-linked RNA-protein complexes by phenol-toluol extraction
description	RNA binding proteins are important regulators of RNA function. Here the authors describe a method for isolation of RNA-protein complexes that does not rely on a specific RNA sequence or motif, and demonstrate the approach by providing the global RNA-bound proteomes of human HEK293 cells and Salmonella Typhimurium.
format	article
author	Erika C. Urdaneta Carlos H. Vieira-Vieira Timon Hick Hans-Herrmann Wessels Davide Figini Rebecca Moschall Jan Medenbach Uwe Ohler Sander Granneman Matthias Selbach Benedikt M. Beckmann
author_facet	Erika C. Urdaneta Carlos H. Vieira-Vieira Timon Hick Hans-Herrmann Wessels Davide Figini Rebecca Moschall Jan Medenbach Uwe Ohler Sander Granneman Matthias Selbach Benedikt M. Beckmann
author_sort	Erika C. Urdaneta
title	Purification of cross-linked RNA-protein complexes by phenol-toluol extraction
title_short	Purification of cross-linked RNA-protein complexes by phenol-toluol extraction
title_full	Purification of cross-linked RNA-protein complexes by phenol-toluol extraction
title_fullStr	Purification of cross-linked RNA-protein complexes by phenol-toluol extraction
title_full_unstemmed	Purification of cross-linked RNA-protein complexes by phenol-toluol extraction
title_sort	purification of cross-linked rna-protein complexes by phenol-toluol extraction
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/4c172901827e4b4c9950ae7fba3c2b89
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Purification of cross-linked RNA-protein complexes by phenol-toluol extraction

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