Genome sequencing of the multicellular alga Astrephomene provides insights into convergent evolution of germ-soma differentiation

Abstract Germ-soma differentiation evolved independently in many eukaryotic lineages and contributed to complex multicellular organizations. However, the molecular genetic bases of such convergent evolution remain unresolved. Two multicellular volvocine green algae, Volvox and Astrephomene, exhibit...

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Autores principales:	Shota Yamashita, Kayoko Yamamoto, Ryo Matsuzaki, Shigekatsu Suzuki, Haruyo Yamaguchi, Shunsuke Hirooka, Yohei Minakuchi, Shin-ya Miyagishima, Masanobu Kawachi, Atsushi Toyoda, Hisayoshi Nozaki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4c38e1570fdd4a0ab5afa34fa4462dd9
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spelling	oai:doaj.org-article:4c38e1570fdd4a0ab5afa34fa4462dd92021-11-28T12:21:21ZGenome sequencing of the multicellular alga Astrephomene provides insights into convergent evolution of germ-soma differentiation10.1038/s41598-021-01521-x2045-2322https://doaj.org/article/4c38e1570fdd4a0ab5afa34fa4462dd92021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01521-xhttps://doaj.org/toc/2045-2322Abstract Germ-soma differentiation evolved independently in many eukaryotic lineages and contributed to complex multicellular organizations. However, the molecular genetic bases of such convergent evolution remain unresolved. Two multicellular volvocine green algae, Volvox and Astrephomene, exhibit convergent evolution of germ-soma differentiation. The complete genome sequence is now available for Volvox, while genome information is scarce for Astrephomene. Here, we generated the de novo whole genome sequence of Astrephomene gubernaculifera and conducted RNA-seq analysis of isolated somatic and reproductive cells. In Volvox, tandem duplication and neofunctionalization of the ancestral transcription factor gene (RLS1/rlsD) might have led to the evolution of regA, the master regulator for Volvox germ-soma differentiation. However, our genome data demonstrated that Astrephomene has not undergone tandem duplication of the RLS1/rlsD homolog or acquisition of a regA-like gene. Our RNA-seq analysis revealed the downregulation of photosynthetic and anabolic gene expression in Astrephomene somatic cells, as in Volvox. Among genes with high expression in somatic cells of Astrephomene, we identified three genes encoding putative transcription factors, which may regulate somatic cell differentiation. Thus, the convergent evolution of germ-soma differentiation in the volvocine algae may have occurred by the acquisition of different regulatory circuits that generate a similar division of labor.Shota YamashitaKayoko YamamotoRyo MatsuzakiShigekatsu SuzukiHaruyo YamaguchiShunsuke HirookaYohei MinakuchiShin-ya MiyagishimaMasanobu KawachiAtsushi ToyodaHisayoshi NozakiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shota Yamashita Kayoko Yamamoto Ryo Matsuzaki Shigekatsu Suzuki Haruyo Yamaguchi Shunsuke Hirooka Yohei Minakuchi Shin-ya Miyagishima Masanobu Kawachi Atsushi Toyoda Hisayoshi Nozaki Genome sequencing of the multicellular alga Astrephomene provides insights into convergent evolution of germ-soma differentiation
description	Abstract Germ-soma differentiation evolved independently in many eukaryotic lineages and contributed to complex multicellular organizations. However, the molecular genetic bases of such convergent evolution remain unresolved. Two multicellular volvocine green algae, Volvox and Astrephomene, exhibit convergent evolution of germ-soma differentiation. The complete genome sequence is now available for Volvox, while genome information is scarce for Astrephomene. Here, we generated the de novo whole genome sequence of Astrephomene gubernaculifera and conducted RNA-seq analysis of isolated somatic and reproductive cells. In Volvox, tandem duplication and neofunctionalization of the ancestral transcription factor gene (RLS1/rlsD) might have led to the evolution of regA, the master regulator for Volvox germ-soma differentiation. However, our genome data demonstrated that Astrephomene has not undergone tandem duplication of the RLS1/rlsD homolog or acquisition of a regA-like gene. Our RNA-seq analysis revealed the downregulation of photosynthetic and anabolic gene expression in Astrephomene somatic cells, as in Volvox. Among genes with high expression in somatic cells of Astrephomene, we identified three genes encoding putative transcription factors, which may regulate somatic cell differentiation. Thus, the convergent evolution of germ-soma differentiation in the volvocine algae may have occurred by the acquisition of different regulatory circuits that generate a similar division of labor.
format	article
author	Shota Yamashita Kayoko Yamamoto Ryo Matsuzaki Shigekatsu Suzuki Haruyo Yamaguchi Shunsuke Hirooka Yohei Minakuchi Shin-ya Miyagishima Masanobu Kawachi Atsushi Toyoda Hisayoshi Nozaki
author_facet	Shota Yamashita Kayoko Yamamoto Ryo Matsuzaki Shigekatsu Suzuki Haruyo Yamaguchi Shunsuke Hirooka Yohei Minakuchi Shin-ya Miyagishima Masanobu Kawachi Atsushi Toyoda Hisayoshi Nozaki
author_sort	Shota Yamashita
title	Genome sequencing of the multicellular alga Astrephomene provides insights into convergent evolution of germ-soma differentiation
title_short	Genome sequencing of the multicellular alga Astrephomene provides insights into convergent evolution of germ-soma differentiation
title_full	Genome sequencing of the multicellular alga Astrephomene provides insights into convergent evolution of germ-soma differentiation
title_fullStr	Genome sequencing of the multicellular alga Astrephomene provides insights into convergent evolution of germ-soma differentiation
title_full_unstemmed	Genome sequencing of the multicellular alga Astrephomene provides insights into convergent evolution of germ-soma differentiation
title_sort	genome sequencing of the multicellular alga astrephomene provides insights into convergent evolution of germ-soma differentiation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4c38e1570fdd4a0ab5afa34fa4462dd9
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Genome sequencing of the multicellular alga Astrephomene provides insights into convergent evolution of germ-soma differentiation

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