Modeling and live imaging of mechanical instabilities in the zebrafish aorta during hematopoiesis

Abstract All blood cells originate from hematopoietic stem/progenitor cells (HSPCs). HSPCs are formed from endothelial cells (ECs) of the dorsal aorta (DA), via endothelial-to-hematopoietic transition (EHT). The zebrafish is a primary model organism to study the process in vivo. While the role of me...

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Autores principales:	Dmitrii Chalin, Charlotte Bureau, Andrea Parmeggiani, Sergei Rochal, Karima Kissa, Ivan Golushko
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4c755f40f6754e448911725955fc6061
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spelling	oai:doaj.org-article:4c755f40f6754e448911725955fc60612021-12-02T17:39:32ZModeling and live imaging of mechanical instabilities in the zebrafish aorta during hematopoiesis10.1038/s41598-021-88667-w2045-2322https://doaj.org/article/4c755f40f6754e448911725955fc60612021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88667-whttps://doaj.org/toc/2045-2322Abstract All blood cells originate from hematopoietic stem/progenitor cells (HSPCs). HSPCs are formed from endothelial cells (ECs) of the dorsal aorta (DA), via endothelial-to-hematopoietic transition (EHT). The zebrafish is a primary model organism to study the process in vivo. While the role of mechanical stress in controlling gene expression promoting cell differentiation is actively investigated, mechanisms driving shape changes of the DA and individual ECs remain poorly understood. We address this problem by developing a new DA micromechanical model and applying it to experimental data on zebrafish morphogenesis. The model considers the DA as an isotropic tubular membrane subjected to hydrostatic blood pressure and axial stress. The DA evolution is described as a movement in the dimensionless controlling parameters space: normalized hydrostatic pressure and axial stress. We argue that HSPC production is accompanied by two mechanical instabilities arising in the system due to the plane stress in the DA walls and show how a complex interplay between mechanical forces in the system drives the emerging morphological changes.Dmitrii ChalinCharlotte BureauAndrea ParmeggianiSergei RochalKarima KissaIvan GolushkoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dmitrii Chalin Charlotte Bureau Andrea Parmeggiani Sergei Rochal Karima Kissa Ivan Golushko Modeling and live imaging of mechanical instabilities in the zebrafish aorta during hematopoiesis
description	Abstract All blood cells originate from hematopoietic stem/progenitor cells (HSPCs). HSPCs are formed from endothelial cells (ECs) of the dorsal aorta (DA), via endothelial-to-hematopoietic transition (EHT). The zebrafish is a primary model organism to study the process in vivo. While the role of mechanical stress in controlling gene expression promoting cell differentiation is actively investigated, mechanisms driving shape changes of the DA and individual ECs remain poorly understood. We address this problem by developing a new DA micromechanical model and applying it to experimental data on zebrafish morphogenesis. The model considers the DA as an isotropic tubular membrane subjected to hydrostatic blood pressure and axial stress. The DA evolution is described as a movement in the dimensionless controlling parameters space: normalized hydrostatic pressure and axial stress. We argue that HSPC production is accompanied by two mechanical instabilities arising in the system due to the plane stress in the DA walls and show how a complex interplay between mechanical forces in the system drives the emerging morphological changes.
format	article
author	Dmitrii Chalin Charlotte Bureau Andrea Parmeggiani Sergei Rochal Karima Kissa Ivan Golushko
author_facet	Dmitrii Chalin Charlotte Bureau Andrea Parmeggiani Sergei Rochal Karima Kissa Ivan Golushko
author_sort	Dmitrii Chalin
title	Modeling and live imaging of mechanical instabilities in the zebrafish aorta during hematopoiesis
title_short	Modeling and live imaging of mechanical instabilities in the zebrafish aorta during hematopoiesis
title_full	Modeling and live imaging of mechanical instabilities in the zebrafish aorta during hematopoiesis
title_fullStr	Modeling and live imaging of mechanical instabilities in the zebrafish aorta during hematopoiesis
title_full_unstemmed	Modeling and live imaging of mechanical instabilities in the zebrafish aorta during hematopoiesis
title_sort	modeling and live imaging of mechanical instabilities in the zebrafish aorta during hematopoiesis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4c755f40f6754e448911725955fc6061
work_keys_str_mv	AT dmitriichalin modelingandliveimagingofmechanicalinstabilitiesinthezebrafishaortaduringhematopoiesis AT charlottebureau modelingandliveimagingofmechanicalinstabilitiesinthezebrafishaortaduringhematopoiesis AT andreaparmeggiani modelingandliveimagingofmechanicalinstabilitiesinthezebrafishaortaduringhematopoiesis AT sergeirochal modelingandliveimagingofmechanicalinstabilitiesinthezebrafishaortaduringhematopoiesis AT karimakissa modelingandliveimagingofmechanicalinstabilitiesinthezebrafishaortaduringhematopoiesis AT ivangolushko modelingandliveimagingofmechanicalinstabilitiesinthezebrafishaortaduringhematopoiesis
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Modeling and live imaging of mechanical instabilities in the zebrafish aorta during hematopoiesis

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