Light microscopy of proteins in their ultrastructural context

Imaging specific proteins in the ultrastructural context largely relies on correlative light/electron microscopy, but fluorophore incompatibility and registration issues limit its use. Here the authors develop an expansion microscopy method with pan-labeling of the proteome to obtain EM-equivalent l...

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Autores principales:	Ons M’Saad, Joerg Bewersdorf
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/4d8ba5c29a00481891dbe1df9367f3e0
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spelling	oai:doaj.org-article:4d8ba5c29a00481891dbe1df9367f3e02021-12-02T16:13:57ZLight microscopy of proteins in their ultrastructural context10.1038/s41467-020-17523-82041-1723https://doaj.org/article/4d8ba5c29a00481891dbe1df9367f3e02020-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17523-8https://doaj.org/toc/2041-1723Imaging specific proteins in the ultrastructural context largely relies on correlative light/electron microscopy, but fluorophore incompatibility and registration issues limit its use. Here the authors develop an expansion microscopy method with pan-labeling of the proteome to obtain EM-equivalent light microscopy images.Ons M’SaadJoerg BewersdorfNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-15 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Ons M’Saad Joerg Bewersdorf Light microscopy of proteins in their ultrastructural context
description	Imaging specific proteins in the ultrastructural context largely relies on correlative light/electron microscopy, but fluorophore incompatibility and registration issues limit its use. Here the authors develop an expansion microscopy method with pan-labeling of the proteome to obtain EM-equivalent light microscopy images.
format	article
author	Ons M’Saad Joerg Bewersdorf
author_facet	Ons M’Saad Joerg Bewersdorf
author_sort	Ons M’Saad
title	Light microscopy of proteins in their ultrastructural context
title_short	Light microscopy of proteins in their ultrastructural context
title_full	Light microscopy of proteins in their ultrastructural context
title_fullStr	Light microscopy of proteins in their ultrastructural context
title_full_unstemmed	Light microscopy of proteins in their ultrastructural context
title_sort	light microscopy of proteins in their ultrastructural context
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/4d8ba5c29a00481891dbe1df9367f3e0
work_keys_str_mv	AT onsmsaad lightmicroscopyofproteinsintheirultrastructuralcontext AT joergbewersdorf lightmicroscopyofproteinsintheirultrastructuralcontext
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Light microscopy of proteins in their ultrastructural context

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