Single-cell barcode analysis provides a rapid readout of cellular signaling pathways in clinical specimens

Diagnostic tests need optimization to avoid invasive and costly repeated biopsies. Here the authors present an antibody-DNA barcoding approach where harvested single cells can be re-stained through custom oligonucleotide-fluorophore conjugates, enabling multiplexed analysis of cancer pathways.

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Auteurs principaux:	Randy J. Giedt, Divya Pathania, Jonathan C. T. Carlson, Philip J. McFarland, Andres Fernandez del Castillo, Dejan Juric, Ralph Weissleder
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2018
Sujets:	Science Q
Accès en ligne:	https://doaj.org/article/4da7f9223b3c49949e8bbac7d3b4f4f6
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spelling	oai:doaj.org-article:4da7f9223b3c49949e8bbac7d3b4f4f62021-12-02T15:33:55ZSingle-cell barcode analysis provides a rapid readout of cellular signaling pathways in clinical specimens10.1038/s41467-018-07002-62041-1723https://doaj.org/article/4da7f9223b3c49949e8bbac7d3b4f4f62018-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-07002-6https://doaj.org/toc/2041-1723Diagnostic tests need optimization to avoid invasive and costly repeated biopsies. Here the authors present an antibody-DNA barcoding approach where harvested single cells can be re-stained through custom oligonucleotide-fluorophore conjugates, enabling multiplexed analysis of cancer pathways.Randy J. GiedtDivya PathaniaJonathan C. T. CarlsonPhilip J. McFarlandAndres Fernandez del CastilloDejan JuricRalph WeisslederNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Randy J. Giedt Divya Pathania Jonathan C. T. Carlson Philip J. McFarland Andres Fernandez del Castillo Dejan Juric Ralph Weissleder Single-cell barcode analysis provides a rapid readout of cellular signaling pathways in clinical specimens
description	Diagnostic tests need optimization to avoid invasive and costly repeated biopsies. Here the authors present an antibody-DNA barcoding approach where harvested single cells can be re-stained through custom oligonucleotide-fluorophore conjugates, enabling multiplexed analysis of cancer pathways.
format	article
author	Randy J. Giedt Divya Pathania Jonathan C. T. Carlson Philip J. McFarland Andres Fernandez del Castillo Dejan Juric Ralph Weissleder
author_facet	Randy J. Giedt Divya Pathania Jonathan C. T. Carlson Philip J. McFarland Andres Fernandez del Castillo Dejan Juric Ralph Weissleder
author_sort	Randy J. Giedt
title	Single-cell barcode analysis provides a rapid readout of cellular signaling pathways in clinical specimens
title_short	Single-cell barcode analysis provides a rapid readout of cellular signaling pathways in clinical specimens
title_full	Single-cell barcode analysis provides a rapid readout of cellular signaling pathways in clinical specimens
title_fullStr	Single-cell barcode analysis provides a rapid readout of cellular signaling pathways in clinical specimens
title_full_unstemmed	Single-cell barcode analysis provides a rapid readout of cellular signaling pathways in clinical specimens
title_sort	single-cell barcode analysis provides a rapid readout of cellular signaling pathways in clinical specimens
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/4da7f9223b3c49949e8bbac7d3b4f4f6
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Single-cell barcode analysis provides a rapid readout of cellular signaling pathways in clinical specimens

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