Single-cell barcode analysis provides a rapid readout of cellular signaling pathways in clinical specimens

Diagnostic tests need optimization to avoid invasive and costly repeated biopsies. Here the authors present an antibody-DNA barcoding approach where harvested single cells can be re-stained through custom oligonucleotide-fluorophore conjugates, enabling multiplexed analysis of cancer pathways.

Guardado en:

Detalles Bibliográficos
Autores principales:	Randy J. Giedt, Divya Pathania, Jonathan C. T. Carlson, Philip J. McFarland, Andres Fernandez del Castillo, Dejan Juric, Ralph Weissleder
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/4da7f9223b3c49949e8bbac7d3b4f4f6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4da7f9223b3c49949e8bbac7d3b4f4f6
record_format	dspace
spelling	oai:doaj.org-article:4da7f9223b3c49949e8bbac7d3b4f4f62021-12-02T15:33:55ZSingle-cell barcode analysis provides a rapid readout of cellular signaling pathways in clinical specimens10.1038/s41467-018-07002-62041-1723https://doaj.org/article/4da7f9223b3c49949e8bbac7d3b4f4f62018-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-07002-6https://doaj.org/toc/2041-1723Diagnostic tests need optimization to avoid invasive and costly repeated biopsies. Here the authors present an antibody-DNA barcoding approach where harvested single cells can be re-stained through custom oligonucleotide-fluorophore conjugates, enabling multiplexed analysis of cancer pathways.Randy J. GiedtDivya PathaniaJonathan C. T. CarlsonPhilip J. McFarlandAndres Fernandez del CastilloDejan JuricRalph WeisslederNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Randy J. Giedt Divya Pathania Jonathan C. T. Carlson Philip J. McFarland Andres Fernandez del Castillo Dejan Juric Ralph Weissleder Single-cell barcode analysis provides a rapid readout of cellular signaling pathways in clinical specimens
description	Diagnostic tests need optimization to avoid invasive and costly repeated biopsies. Here the authors present an antibody-DNA barcoding approach where harvested single cells can be re-stained through custom oligonucleotide-fluorophore conjugates, enabling multiplexed analysis of cancer pathways.
format	article
author	Randy J. Giedt Divya Pathania Jonathan C. T. Carlson Philip J. McFarland Andres Fernandez del Castillo Dejan Juric Ralph Weissleder
author_facet	Randy J. Giedt Divya Pathania Jonathan C. T. Carlson Philip J. McFarland Andres Fernandez del Castillo Dejan Juric Ralph Weissleder
author_sort	Randy J. Giedt
title	Single-cell barcode analysis provides a rapid readout of cellular signaling pathways in clinical specimens
title_short	Single-cell barcode analysis provides a rapid readout of cellular signaling pathways in clinical specimens
title_full	Single-cell barcode analysis provides a rapid readout of cellular signaling pathways in clinical specimens
title_fullStr	Single-cell barcode analysis provides a rapid readout of cellular signaling pathways in clinical specimens
title_full_unstemmed	Single-cell barcode analysis provides a rapid readout of cellular signaling pathways in clinical specimens
title_sort	single-cell barcode analysis provides a rapid readout of cellular signaling pathways in clinical specimens
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/4da7f9223b3c49949e8bbac7d3b4f4f6
work_keys_str_mv	AT randyjgiedt singlecellbarcodeanalysisprovidesarapidreadoutofcellularsignalingpathwaysinclinicalspecimens AT divyapathania singlecellbarcodeanalysisprovidesarapidreadoutofcellularsignalingpathwaysinclinicalspecimens AT jonathanctcarlson singlecellbarcodeanalysisprovidesarapidreadoutofcellularsignalingpathwaysinclinicalspecimens AT philipjmcfarland singlecellbarcodeanalysisprovidesarapidreadoutofcellularsignalingpathwaysinclinicalspecimens AT andresfernandezdelcastillo singlecellbarcodeanalysisprovidesarapidreadoutofcellularsignalingpathwaysinclinicalspecimens AT dejanjuric singlecellbarcodeanalysisprovidesarapidreadoutofcellularsignalingpathwaysinclinicalspecimens AT ralphweissleder singlecellbarcodeanalysisprovidesarapidreadoutofcellularsignalingpathwaysinclinicalspecimens
_version_	1718386958491713536

Single-cell barcode analysis provides a rapid readout of cellular signaling pathways in clinical specimens

Ejemplares similares