Fabrication of a paper-based colorimetric molecular test for SARS-CoV-2

The ongoing pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused unprecedented damage to the global economy. Diagnostic testing is a key factor in limiting virus transmission and safeguarding public health. We present the fabrication process of a paper-based dev...

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Autores principales:	Jiangshan Wang, Andres Dextre, Ana Pascual-Garrigos, Josiah Levi Davidson, Darby McChesney, Jordan Seville, Mohit S. Verma
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Paper-based colorimetric LAMP Science Q
Acceso en línea:	https://doaj.org/article/4dfefa5b7c7b41988312586a2eb72970
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spelling	oai:doaj.org-article:4dfefa5b7c7b41988312586a2eb729702021-11-26T04:30:09ZFabrication of a paper-based colorimetric molecular test for SARS-CoV-22215-016110.1016/j.mex.2021.101586https://doaj.org/article/4dfefa5b7c7b41988312586a2eb729702021-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2215016121003769https://doaj.org/toc/2215-0161The ongoing pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused unprecedented damage to the global economy. Diagnostic testing is a key factor in limiting virus transmission and safeguarding public health. We present the fabrication process of a paper-based device that uses reverse-transcription loop-mediated isothermal amplification (RT-LAMP) to detect SARS-CoV-2 in complex matrices by providing a colorimetric response apparent to the naked eye. Because of LAMP's functionality, this device just requires a simple heat source (e.g., water bath, incubator), it can be deployed in resource-constrained areas and can be used as a supplement to current point-of-care (POC) and community testing procedures. Since the test is based on nucleic acids, the testing platform itself lends to further applications including food safety monitoring, animal diagnostics, etc. simply by changing the specific primers. • We developed a platform capable of on-paper detection of SARS-CoV-2 using colorimetric reporters that produce responses visible to the naked eye. • The platform is easily reconfigurable to target different pathogens by changing the primer sets, and multiplexing is possible by adding additional reaction sites to the device.Jiangshan WangAndres DextreAna Pascual-GarrigosJosiah Levi DavidsonDarby McChesneyJordan SevilleMohit S. VermaElsevierarticlePaper-based colorimetric LAMPScienceQENMethodsX, Vol 8, Iss , Pp 101586- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Paper-based colorimetric LAMP Science Q
spellingShingle	Paper-based colorimetric LAMP Science Q Jiangshan Wang Andres Dextre Ana Pascual-Garrigos Josiah Levi Davidson Darby McChesney Jordan Seville Mohit S. Verma Fabrication of a paper-based colorimetric molecular test for SARS-CoV-2
description	The ongoing pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused unprecedented damage to the global economy. Diagnostic testing is a key factor in limiting virus transmission and safeguarding public health. We present the fabrication process of a paper-based device that uses reverse-transcription loop-mediated isothermal amplification (RT-LAMP) to detect SARS-CoV-2 in complex matrices by providing a colorimetric response apparent to the naked eye. Because of LAMP's functionality, this device just requires a simple heat source (e.g., water bath, incubator), it can be deployed in resource-constrained areas and can be used as a supplement to current point-of-care (POC) and community testing procedures. Since the test is based on nucleic acids, the testing platform itself lends to further applications including food safety monitoring, animal diagnostics, etc. simply by changing the specific primers. • We developed a platform capable of on-paper detection of SARS-CoV-2 using colorimetric reporters that produce responses visible to the naked eye. • The platform is easily reconfigurable to target different pathogens by changing the primer sets, and multiplexing is possible by adding additional reaction sites to the device.
format	article
author	Jiangshan Wang Andres Dextre Ana Pascual-Garrigos Josiah Levi Davidson Darby McChesney Jordan Seville Mohit S. Verma
author_facet	Jiangshan Wang Andres Dextre Ana Pascual-Garrigos Josiah Levi Davidson Darby McChesney Jordan Seville Mohit S. Verma
author_sort	Jiangshan Wang
title	Fabrication of a paper-based colorimetric molecular test for SARS-CoV-2
title_short	Fabrication of a paper-based colorimetric molecular test for SARS-CoV-2
title_full	Fabrication of a paper-based colorimetric molecular test for SARS-CoV-2
title_fullStr	Fabrication of a paper-based colorimetric molecular test for SARS-CoV-2
title_full_unstemmed	Fabrication of a paper-based colorimetric molecular test for SARS-CoV-2
title_sort	fabrication of a paper-based colorimetric molecular test for sars-cov-2
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/4dfefa5b7c7b41988312586a2eb72970
work_keys_str_mv	AT jiangshanwang fabricationofapaperbasedcolorimetricmoleculartestforsarscov2 AT andresdextre fabricationofapaperbasedcolorimetricmoleculartestforsarscov2 AT anapascualgarrigos fabricationofapaperbasedcolorimetricmoleculartestforsarscov2 AT josiahlevidavidson fabricationofapaperbasedcolorimetricmoleculartestforsarscov2 AT darbymcchesney fabricationofapaperbasedcolorimetricmoleculartestforsarscov2 AT jordanseville fabricationofapaperbasedcolorimetricmoleculartestforsarscov2 AT mohitsverma fabricationofapaperbasedcolorimetricmoleculartestforsarscov2
_version_	1718409840113483776

Fabrication of a paper-based colorimetric molecular test for SARS-CoV-2

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