An Integrative Transcriptomic and Metabolomic Analysis of Red Pitaya (<i>Hylocereus polyrhizus</i>) Seedlings in Response to Heat Stress

Red pitaya (<i>Hylocereus polyrhizus</i>) is a significant functional food that is largely planted in Southeast Asia. Heat stress (HS) induced by high temperatures is likely to restrict the growth and survival of red pitaya. Although pitaya can tolerate temperatures as high as 40 °C, lit...

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Autores principales:	Zhengli Jiao, Weijuan Xu, Quandong Nong, Mei Zhang, Shuguang Jian, Hongfang Lu, Jiantong Chen, Mingyong Zhang, Kuaifei Xia
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	red pitaya heat stress transcriptome metabolome PR-1 protein Genetics QH426-470
Acceso en línea:	https://doaj.org/article/4f9fc5ee618d406a9036328460a3fc18
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spelling	oai:doaj.org-article:4f9fc5ee618d406a9036328460a3fc182021-11-25T17:41:11ZAn Integrative Transcriptomic and Metabolomic Analysis of Red Pitaya (<i>Hylocereus polyrhizus</i>) Seedlings in Response to Heat Stress10.3390/genes121117142073-4425https://doaj.org/article/4f9fc5ee618d406a9036328460a3fc182021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1714https://doaj.org/toc/2073-4425Red pitaya (<i>Hylocereus polyrhizus</i>) is a significant functional food that is largely planted in Southeast Asia. Heat stress (HS) induced by high temperatures is likely to restrict the growth and survival of red pitaya. Although pitaya can tolerate temperatures as high as 40 °C, little is known of how it can withstand HS. In this study, the transcriptomic and metabolomic responses of red pitaya seedlings to HS were analyzed. A total of 198 transcripts (122 upregulated and 76 downregulated) were significantly differentially expressed after 24 h and 72 h of exposure to 42 °C compared with a control grown at 28 °C. We also identified 64 differentially accumulated metabolites in pitaya under HS (37 increased and 27 decreased). These differential metabolites, especially amino acids, organic acids, and sugars, are involved in metabolic pathways and the biosynthesis of amino acids. Interaction network analysis of the heat-responsive genes and metabolites suggested that similar pathways and complex response mechanisms are involved in the response of pitaya to HS. Overexpression of one of the upregulated genes (<i>contig10820</i>) in <i>Arabidopsis</i>, which is a homolog of <i>PR-1</i> and named <i>HuPR-1</i>, significantly increased tolerance to HS. This is the first study showing that <i>HuPR-1</i> plays a role in the response of pitaya to abiotic stress. These findings provide valuable insights that will aid future studies examining adaptation to HS in pitaya.Zhengli JiaoWeijuan XuQuandong NongMei ZhangShuguang JianHongfang LuJiantong ChenMingyong ZhangKuaifei XiaMDPI AGarticlered pitayaheat stresstranscriptomemetabolomePR-1 proteinGeneticsQH426-470ENGenes, Vol 12, Iss 1714, p 1714 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	red pitaya heat stress transcriptome metabolome PR-1 protein Genetics QH426-470
spellingShingle	red pitaya heat stress transcriptome metabolome PR-1 protein Genetics QH426-470 Zhengli Jiao Weijuan Xu Quandong Nong Mei Zhang Shuguang Jian Hongfang Lu Jiantong Chen Mingyong Zhang Kuaifei Xia An Integrative Transcriptomic and Metabolomic Analysis of Red Pitaya (<i>Hylocereus polyrhizus</i>) Seedlings in Response to Heat Stress
description	Red pitaya (<i>Hylocereus polyrhizus</i>) is a significant functional food that is largely planted in Southeast Asia. Heat stress (HS) induced by high temperatures is likely to restrict the growth and survival of red pitaya. Although pitaya can tolerate temperatures as high as 40 °C, little is known of how it can withstand HS. In this study, the transcriptomic and metabolomic responses of red pitaya seedlings to HS were analyzed. A total of 198 transcripts (122 upregulated and 76 downregulated) were significantly differentially expressed after 24 h and 72 h of exposure to 42 °C compared with a control grown at 28 °C. We also identified 64 differentially accumulated metabolites in pitaya under HS (37 increased and 27 decreased). These differential metabolites, especially amino acids, organic acids, and sugars, are involved in metabolic pathways and the biosynthesis of amino acids. Interaction network analysis of the heat-responsive genes and metabolites suggested that similar pathways and complex response mechanisms are involved in the response of pitaya to HS. Overexpression of one of the upregulated genes (<i>contig10820</i>) in <i>Arabidopsis</i>, which is a homolog of <i>PR-1</i> and named <i>HuPR-1</i>, significantly increased tolerance to HS. This is the first study showing that <i>HuPR-1</i> plays a role in the response of pitaya to abiotic stress. These findings provide valuable insights that will aid future studies examining adaptation to HS in pitaya.
format	article
author	Zhengli Jiao Weijuan Xu Quandong Nong Mei Zhang Shuguang Jian Hongfang Lu Jiantong Chen Mingyong Zhang Kuaifei Xia
author_facet	Zhengli Jiao Weijuan Xu Quandong Nong Mei Zhang Shuguang Jian Hongfang Lu Jiantong Chen Mingyong Zhang Kuaifei Xia
author_sort	Zhengli Jiao
title	An Integrative Transcriptomic and Metabolomic Analysis of Red Pitaya (<i>Hylocereus polyrhizus</i>) Seedlings in Response to Heat Stress
title_short	An Integrative Transcriptomic and Metabolomic Analysis of Red Pitaya (<i>Hylocereus polyrhizus</i>) Seedlings in Response to Heat Stress
title_full	An Integrative Transcriptomic and Metabolomic Analysis of Red Pitaya (<i>Hylocereus polyrhizus</i>) Seedlings in Response to Heat Stress
title_fullStr	An Integrative Transcriptomic and Metabolomic Analysis of Red Pitaya (<i>Hylocereus polyrhizus</i>) Seedlings in Response to Heat Stress
title_full_unstemmed	An Integrative Transcriptomic and Metabolomic Analysis of Red Pitaya (<i>Hylocereus polyrhizus</i>) Seedlings in Response to Heat Stress
title_sort	integrative transcriptomic and metabolomic analysis of red pitaya (<i>hylocereus polyrhizus</i>) seedlings in response to heat stress
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4f9fc5ee618d406a9036328460a3fc18
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An Integrative Transcriptomic and Metabolomic Analysis of Red Pitaya (<i>Hylocereus polyrhizus</i>) Seedlings in Response to Heat Stress

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