DETERMINATION OF PHENOLIC CONTENT AND EVALUATION OF ANTIOXIDANT ACTIVITY OF PROPOLIS FROM ANTIOQUIA (COLOMBIA)

Propolis, a natural product collected by honeybee from exudates of plants, is widely used in traditional medicine due to its known therapeutic properties. In this paper, the quality of ethanol extracts of propolis (EEP) from different regions of Antioquia (Colombia) is compared through the determin...

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Autores principales:	Lady R. PALOMINO G., Carlos M. GARCÍA P., Jesús H. GIL G., Benjamín A. ROJANO, Diego L. DURANGO R.
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2009
Materias:	propolis phenolic compounds flavonoids DPPH FRAP. Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
Acceso en línea:	https://doaj.org/article/50a19572673a4dd0bcd527630104f492
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spelling	oai:doaj.org-article:50a19572673a4dd0bcd527630104f4922021-11-19T04:14:49ZDETERMINATION OF PHENOLIC CONTENT AND EVALUATION OF ANTIOXIDANT ACTIVITY OF PROPOLIS FROM ANTIOQUIA (COLOMBIA)0121-40042145-2660https://doaj.org/article/50a19572673a4dd0bcd527630104f4922009-11-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/vitae/article/view/3020https://doaj.org/toc/0121-4004https://doaj.org/toc/2145-2660 Propolis, a natural product collected by honeybee from exudates of plants, is widely used in traditional medicine due to its known therapeutic properties. In this paper, the quality of ethanol extracts of propolis (EEP) from different regions of Antioquia (Colombia) is compared through the determination of flavonoids and total phenolic compounds content, and thein vitro antioxidant activity, using three assay systems: radical scavenging activity by means of the DPPH• (1-diphenyl-2-picrylhydrazyl) and ABTS•+ (2,2’-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid cation) assays, and ferric reducing antioxidant power (FRAP). Determinations of total phenolic compounds content are found between 22.11 ± 0.54 and 75.22 ± 1.35 mg GAE/g of EEP, and total content of flavonoids between 4.75 ± 0.01 y 34.50 ± 0.07 mg QE/g of EEP. The radical scavenging activity varies from 33.9 ± 9.7 to 324.6 ± 15.0, and from 455.5 ± 7.8 to 1091 ± 17.3 μmol TE/g of EEP (TEAC) in the DPPH and ABTS system, respectively. In the FRAP method, the activity is found between 40.9 ± 13.3 and 338.4 ± 22.4 μmol AAE/g of EEP (AEAC). Results show a positive linear correlation between antioxidant activity and total phenolic content. The antioxidant activity of some propolis indicates its potential role as nutraceutical. Lady R. PALOMINO G.Carlos M. GARCÍA P.Jesús H. GIL G.Benjamín A. ROJANODiego L. DURANGO R.Universidad de Antioquiaarticlepropolisphenolic compoundsflavonoidsDPPHFRAP.Food processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 16, Iss 3 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	propolis phenolic compounds flavonoids DPPH FRAP. Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
spellingShingle	propolis phenolic compounds flavonoids DPPH FRAP. Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675 Lady R. PALOMINO G. Carlos M. GARCÍA P. Jesús H. GIL G. Benjamín A. ROJANO Diego L. DURANGO R. DETERMINATION OF PHENOLIC CONTENT AND EVALUATION OF ANTIOXIDANT ACTIVITY OF PROPOLIS FROM ANTIOQUIA (COLOMBIA)
description	Propolis, a natural product collected by honeybee from exudates of plants, is widely used in traditional medicine due to its known therapeutic properties. In this paper, the quality of ethanol extracts of propolis (EEP) from different regions of Antioquia (Colombia) is compared through the determination of flavonoids and total phenolic compounds content, and thein vitro antioxidant activity, using three assay systems: radical scavenging activity by means of the DPPH• (1-diphenyl-2-picrylhydrazyl) and ABTS•+ (2,2’-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid cation) assays, and ferric reducing antioxidant power (FRAP). Determinations of total phenolic compounds content are found between 22.11 ± 0.54 and 75.22 ± 1.35 mg GAE/g of EEP, and total content of flavonoids between 4.75 ± 0.01 y 34.50 ± 0.07 mg QE/g of EEP. The radical scavenging activity varies from 33.9 ± 9.7 to 324.6 ± 15.0, and from 455.5 ± 7.8 to 1091 ± 17.3 μmol TE/g of EEP (TEAC) in the DPPH and ABTS system, respectively. In the FRAP method, the activity is found between 40.9 ± 13.3 and 338.4 ± 22.4 μmol AAE/g of EEP (AEAC). Results show a positive linear correlation between antioxidant activity and total phenolic content. The antioxidant activity of some propolis indicates its potential role as nutraceutical.
format	article
author	Lady R. PALOMINO G. Carlos M. GARCÍA P. Jesús H. GIL G. Benjamín A. ROJANO Diego L. DURANGO R.
author_facet	Lady R. PALOMINO G. Carlos M. GARCÍA P. Jesús H. GIL G. Benjamín A. ROJANO Diego L. DURANGO R.
author_sort	Lady R. PALOMINO G.
title	DETERMINATION OF PHENOLIC CONTENT AND EVALUATION OF ANTIOXIDANT ACTIVITY OF PROPOLIS FROM ANTIOQUIA (COLOMBIA)
title_short	DETERMINATION OF PHENOLIC CONTENT AND EVALUATION OF ANTIOXIDANT ACTIVITY OF PROPOLIS FROM ANTIOQUIA (COLOMBIA)
title_full	DETERMINATION OF PHENOLIC CONTENT AND EVALUATION OF ANTIOXIDANT ACTIVITY OF PROPOLIS FROM ANTIOQUIA (COLOMBIA)
title_fullStr	DETERMINATION OF PHENOLIC CONTENT AND EVALUATION OF ANTIOXIDANT ACTIVITY OF PROPOLIS FROM ANTIOQUIA (COLOMBIA)
title_full_unstemmed	DETERMINATION OF PHENOLIC CONTENT AND EVALUATION OF ANTIOXIDANT ACTIVITY OF PROPOLIS FROM ANTIOQUIA (COLOMBIA)
title_sort	determination of phenolic content and evaluation of antioxidant activity of propolis from antioquia (colombia)
publisher	Universidad de Antioquia
publishDate	2009
url	https://doaj.org/article/50a19572673a4dd0bcd527630104f492
work_keys_str_mv	AT ladyrpalominog determinationofphenoliccontentandevaluationofantioxidantactivityofpropolisfromantioquiacolombia AT carlosmgarciap determinationofphenoliccontentandevaluationofantioxidantactivityofpropolisfromantioquiacolombia AT jesushgilg determinationofphenoliccontentandevaluationofantioxidantactivityofpropolisfromantioquiacolombia AT benjaminarojano determinationofphenoliccontentandevaluationofantioxidantactivityofpropolisfromantioquiacolombia AT diegoldurangor determinationofphenoliccontentandevaluationofantioxidantactivityofpropolisfromantioquiacolombia
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DETERMINATION OF PHENOLIC CONTENT AND EVALUATION OF ANTIOXIDANT ACTIVITY OF PROPOLIS FROM ANTIOQUIA (COLOMBIA)

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