Multistage hematopoietic stem cell regulation in the mouse: A combined biological and mathematical approach

Summary: We have reconciled steady-state and stress hematopoiesis in a single mathematical model based on murine in vivo experiments and with a focus on hematopoietic stem and progenitor cells. A phenylhydrazine stress was first applied to mice. A reduced cell number in each progenitor compartment w...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Céline Bonnet, Panhong Gou, Simon Girel, Vincent Bansaye, Catherine Lacout, Karine Bailly, Marie-Hélène Schlagetter, Evelyne Lauret, Sylvie Méléard, Stéphane Giraudier
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cell biology Stem cells research In silico biology Science Q
Acceso en línea:	https://doaj.org/article/51509df1070a4d4585faa5eea2a5d7f2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:51509df1070a4d4585faa5eea2a5d7f2
record_format	dspace
spelling	oai:doaj.org-article:51509df1070a4d4585faa5eea2a5d7f22021-11-24T04:33:00ZMultistage hematopoietic stem cell regulation in the mouse: A combined biological and mathematical approach2589-004210.1016/j.isci.2021.103399https://doaj.org/article/51509df1070a4d4585faa5eea2a5d7f22021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589004221013705https://doaj.org/toc/2589-0042Summary: We have reconciled steady-state and stress hematopoiesis in a single mathematical model based on murine in vivo experiments and with a focus on hematopoietic stem and progenitor cells. A phenylhydrazine stress was first applied to mice. A reduced cell number in each progenitor compartment was evidenced during the next 7 days through a drastic level of differentiation without proliferation, followed by a huge proliferative response in all compartments including long-term hematopoietic stem cells, before a return to normal levels. Data analysis led to the addition to the 6-compartment model, of time-dependent regulation that depended indirectly on the compartment sizes. The resulting model was finely calibrated using a stochastic optimization algorithm and could reproduce biological data in silico when applied to different stress conditions (bleeding, chemotherapy, HSC depletion). In conclusion, our multi-step and time-dependent model of immature hematopoiesis provides new avenues to a better understanding of both normal and pathological hematopoiesis.Céline BonnetPanhong GouSimon GirelVincent BansayeCatherine LacoutKarine BaillyMarie-Hélène SchlagetterEvelyne LauretSylvie MéléardStéphane GiraudierElsevierarticleCell biologyStem cells researchIn silico biologyScienceQENiScience, Vol 24, Iss 12, Pp 103399- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cell biology Stem cells research In silico biology Science Q
spellingShingle	Cell biology Stem cells research In silico biology Science Q Céline Bonnet Panhong Gou Simon Girel Vincent Bansaye Catherine Lacout Karine Bailly Marie-Hélène Schlagetter Evelyne Lauret Sylvie Méléard Stéphane Giraudier Multistage hematopoietic stem cell regulation in the mouse: A combined biological and mathematical approach
description	Summary: We have reconciled steady-state and stress hematopoiesis in a single mathematical model based on murine in vivo experiments and with a focus on hematopoietic stem and progenitor cells. A phenylhydrazine stress was first applied to mice. A reduced cell number in each progenitor compartment was evidenced during the next 7 days through a drastic level of differentiation without proliferation, followed by a huge proliferative response in all compartments including long-term hematopoietic stem cells, before a return to normal levels. Data analysis led to the addition to the 6-compartment model, of time-dependent regulation that depended indirectly on the compartment sizes. The resulting model was finely calibrated using a stochastic optimization algorithm and could reproduce biological data in silico when applied to different stress conditions (bleeding, chemotherapy, HSC depletion). In conclusion, our multi-step and time-dependent model of immature hematopoiesis provides new avenues to a better understanding of both normal and pathological hematopoiesis.
format	article
author	Céline Bonnet Panhong Gou Simon Girel Vincent Bansaye Catherine Lacout Karine Bailly Marie-Hélène Schlagetter Evelyne Lauret Sylvie Méléard Stéphane Giraudier
author_facet	Céline Bonnet Panhong Gou Simon Girel Vincent Bansaye Catherine Lacout Karine Bailly Marie-Hélène Schlagetter Evelyne Lauret Sylvie Méléard Stéphane Giraudier
author_sort	Céline Bonnet
title	Multistage hematopoietic stem cell regulation in the mouse: A combined biological and mathematical approach
title_short	Multistage hematopoietic stem cell regulation in the mouse: A combined biological and mathematical approach
title_full	Multistage hematopoietic stem cell regulation in the mouse: A combined biological and mathematical approach
title_fullStr	Multistage hematopoietic stem cell regulation in the mouse: A combined biological and mathematical approach
title_full_unstemmed	Multistage hematopoietic stem cell regulation in the mouse: A combined biological and mathematical approach
title_sort	multistage hematopoietic stem cell regulation in the mouse: a combined biological and mathematical approach
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/51509df1070a4d4585faa5eea2a5d7f2
work_keys_str_mv	AT celinebonnet multistagehematopoieticstemcellregulationinthemouseacombinedbiologicalandmathematicalapproach AT panhonggou multistagehematopoieticstemcellregulationinthemouseacombinedbiologicalandmathematicalapproach AT simongirel multistagehematopoieticstemcellregulationinthemouseacombinedbiologicalandmathematicalapproach AT vincentbansaye multistagehematopoieticstemcellregulationinthemouseacombinedbiologicalandmathematicalapproach AT catherinelacout multistagehematopoieticstemcellregulationinthemouseacombinedbiologicalandmathematicalapproach AT karinebailly multistagehematopoieticstemcellregulationinthemouseacombinedbiologicalandmathematicalapproach AT marieheleneschlagetter multistagehematopoieticstemcellregulationinthemouseacombinedbiologicalandmathematicalapproach AT evelynelauret multistagehematopoieticstemcellregulationinthemouseacombinedbiologicalandmathematicalapproach AT sylviemeleard multistagehematopoieticstemcellregulationinthemouseacombinedbiologicalandmathematicalapproach AT stephanegiraudier multistagehematopoieticstemcellregulationinthemouseacombinedbiologicalandmathematicalapproach
_version_	1718415974334464000

Multistage hematopoietic stem cell regulation in the mouse: A combined biological and mathematical approach

Ejemplares similares