Evaluation of enzymatic activity and identification of potent proteolytic and chitinolytic bacteria isolated from crab shell waste

Abstract. Dliyauddin M, Ardyati T, Suharjono. 2020. Evaluation of enzymatic activity and Identification of Potent Proteolytic and Chitinolytic Bacteria Isolated from Crab Shell Waste. Biodiversitas 21: 211-218. Proteolytic and chitinolytic bacteria are needed to degrade protein-chitin fibers in crab...

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Autores principales:	MOH DLIYAUDDIN, TRI ARDYATI, SUHARJONO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	bacillus cereus, bacillus licheniformis, bacillus subtilis, chitinolytic bacteria, proteolytic bacteria Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/516526cfebec4dadb18807259f838c81
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spelling	oai:doaj.org-article:516526cfebec4dadb18807259f838c812021-11-21T22:09:24ZEvaluation of enzymatic activity and identification of potent proteolytic and chitinolytic bacteria isolated from crab shell waste1412-033X2085-472210.13057/biodiv/d210127https://doaj.org/article/516526cfebec4dadb18807259f838c812019-12-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4670https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Dliyauddin M, Ardyati T, Suharjono. 2020. Evaluation of enzymatic activity and Identification of Potent Proteolytic and Chitinolytic Bacteria Isolated from Crab Shell Waste. Biodiversitas 21: 211-218. Proteolytic and chitinolytic bacteria are needed to degrade protein-chitin fibers in crab shell waste. These bacteria are important for enhancing the degradation rate of crab shell waste in the environment. This study aims to study the enzymatic activity and identify potent proteolytic and chitinolytic bacteria based on 16S rDNA sequences. The bacteria were isolated from crab shell waste using skim milk agar and colloidal chitin agar for proteolytic and chitinolytic bacteria, respectively. The bacterial isolates were screened based on their protease and chitinase activity using casein and chitin as substrates, respectively. The selected isolates were identified based on 16S rDNA sequences similarity. A total of 20 proteolytic and 22 chitinolytic bacteria were successfully isolated from the samples. The isolate of AP9, AP5, and BP14 had a high protease activity. However, only BP14 and CK20 showed a high chitinase activity. The isolate of AP9 was identified as Bacillus subtilis, AP5, and CK20 were identified as Bacillus licheniformis, and BP14 was identified as Bacillus cereus. AP5 dan CK20 were classified as non-pathogenic isolates.MOH DLIYAUDDINTRI ARDYATISUHARJONOMBI & UNS Soloarticlebacillus cereus, bacillus licheniformis, bacillus subtilis, chitinolytic bacteria, proteolytic bacteriaBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 1 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacillus cereus, bacillus licheniformis, bacillus subtilis, chitinolytic bacteria, proteolytic bacteria Biology (General) QH301-705.5
spellingShingle	bacillus cereus, bacillus licheniformis, bacillus subtilis, chitinolytic bacteria, proteolytic bacteria Biology (General) QH301-705.5 MOH DLIYAUDDIN TRI ARDYATI SUHARJONO Evaluation of enzymatic activity and identification of potent proteolytic and chitinolytic bacteria isolated from crab shell waste
description	Abstract. Dliyauddin M, Ardyati T, Suharjono. 2020. Evaluation of enzymatic activity and Identification of Potent Proteolytic and Chitinolytic Bacteria Isolated from Crab Shell Waste. Biodiversitas 21: 211-218. Proteolytic and chitinolytic bacteria are needed to degrade protein-chitin fibers in crab shell waste. These bacteria are important for enhancing the degradation rate of crab shell waste in the environment. This study aims to study the enzymatic activity and identify potent proteolytic and chitinolytic bacteria based on 16S rDNA sequences. The bacteria were isolated from crab shell waste using skim milk agar and colloidal chitin agar for proteolytic and chitinolytic bacteria, respectively. The bacterial isolates were screened based on their protease and chitinase activity using casein and chitin as substrates, respectively. The selected isolates were identified based on 16S rDNA sequences similarity. A total of 20 proteolytic and 22 chitinolytic bacteria were successfully isolated from the samples. The isolate of AP9, AP5, and BP14 had a high protease activity. However, only BP14 and CK20 showed a high chitinase activity. The isolate of AP9 was identified as Bacillus subtilis, AP5, and CK20 were identified as Bacillus licheniformis, and BP14 was identified as Bacillus cereus. AP5 dan CK20 were classified as non-pathogenic isolates.
format	article
author	MOH DLIYAUDDIN TRI ARDYATI SUHARJONO
author_facet	MOH DLIYAUDDIN TRI ARDYATI SUHARJONO
author_sort	MOH DLIYAUDDIN
title	Evaluation of enzymatic activity and identification of potent proteolytic and chitinolytic bacteria isolated from crab shell waste
title_short	Evaluation of enzymatic activity and identification of potent proteolytic and chitinolytic bacteria isolated from crab shell waste
title_full	Evaluation of enzymatic activity and identification of potent proteolytic and chitinolytic bacteria isolated from crab shell waste
title_fullStr	Evaluation of enzymatic activity and identification of potent proteolytic and chitinolytic bacteria isolated from crab shell waste
title_full_unstemmed	Evaluation of enzymatic activity and identification of potent proteolytic and chitinolytic bacteria isolated from crab shell waste
title_sort	evaluation of enzymatic activity and identification of potent proteolytic and chitinolytic bacteria isolated from crab shell waste
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/516526cfebec4dadb18807259f838c81
work_keys_str_mv	AT mohdliyauddin evaluationofenzymaticactivityandidentificationofpotentproteolyticandchitinolyticbacteriaisolatedfromcrabshellwaste AT triardyati evaluationofenzymaticactivityandidentificationofpotentproteolyticandchitinolyticbacteriaisolatedfromcrabshellwaste AT suharjono evaluationofenzymaticactivityandidentificationofpotentproteolyticandchitinolyticbacteriaisolatedfromcrabshellwaste
_version_	1718418581875589120

Evaluation of enzymatic activity and identification of potent proteolytic and chitinolytic bacteria isolated from crab shell waste

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