Mutations Driving Airborne Transmission of A/H5N1 Virus in Mammals Cause Substantial Attenuation in Chickens only when combined

Abstract A/H5N1 influenza viruses pose a threat to human and animal health. A fully avian A/H5N1 influenza virus was previously shown to acquire airborne transmissibility between ferrets upon accumulation of five or six substitutions that affected three traits: polymerase activity, hemagglutinin sta...

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Autores principales:	Mathilde Richard, Sander Herfst, Judith M. A. van den Brand, Dennis de Meulder, Pascal Lexmond, Theo M. Bestebroer, Ron A. M. Fouchier
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/517fb06f055b4f4cae1e52f7a0491796
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spelling	oai:doaj.org-article:517fb06f055b4f4cae1e52f7a04917962021-12-02T15:06:15ZMutations Driving Airborne Transmission of A/H5N1 Virus in Mammals Cause Substantial Attenuation in Chickens only when combined10.1038/s41598-017-07000-62045-2322https://doaj.org/article/517fb06f055b4f4cae1e52f7a04917962017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07000-6https://doaj.org/toc/2045-2322Abstract A/H5N1 influenza viruses pose a threat to human and animal health. A fully avian A/H5N1 influenza virus was previously shown to acquire airborne transmissibility between ferrets upon accumulation of five or six substitutions that affected three traits: polymerase activity, hemagglutinin stability and receptor binding. Here, the impact of these traits on A/H5N1 virus replication, tissue tropism, pathogenesis and transmission was investigated in chickens. The virus containing all substitutions associated with transmission in mammals was highly attenuated in chickens. However, single substitutions that affect polymerase activity, hemagglutinin stability and receptor binding generally had a small or negligible impact on virus replication, morbidity and mortality. A virus carrying two substitutions in the receptor-binding site was attenuated, although its tissue tropism in chickens was not affected. This data indicate that an A/H5N1 virus that is airborne-transmissible between mammals is unlikely to emerge in chickens, although individual mammalian adaptive substitutions have limited impact on viral fitness in chickens.Mathilde RichardSander HerfstJudith M. A. van den BrandDennis de MeulderPascal LexmondTheo M. BestebroerRon A. M. FouchierNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mathilde Richard Sander Herfst Judith M. A. van den Brand Dennis de Meulder Pascal Lexmond Theo M. Bestebroer Ron A. M. Fouchier Mutations Driving Airborne Transmission of A/H5N1 Virus in Mammals Cause Substantial Attenuation in Chickens only when combined
description	Abstract A/H5N1 influenza viruses pose a threat to human and animal health. A fully avian A/H5N1 influenza virus was previously shown to acquire airborne transmissibility between ferrets upon accumulation of five or six substitutions that affected three traits: polymerase activity, hemagglutinin stability and receptor binding. Here, the impact of these traits on A/H5N1 virus replication, tissue tropism, pathogenesis and transmission was investigated in chickens. The virus containing all substitutions associated with transmission in mammals was highly attenuated in chickens. However, single substitutions that affect polymerase activity, hemagglutinin stability and receptor binding generally had a small or negligible impact on virus replication, morbidity and mortality. A virus carrying two substitutions in the receptor-binding site was attenuated, although its tissue tropism in chickens was not affected. This data indicate that an A/H5N1 virus that is airborne-transmissible between mammals is unlikely to emerge in chickens, although individual mammalian adaptive substitutions have limited impact on viral fitness in chickens.
format	article
author	Mathilde Richard Sander Herfst Judith M. A. van den Brand Dennis de Meulder Pascal Lexmond Theo M. Bestebroer Ron A. M. Fouchier
author_facet	Mathilde Richard Sander Herfst Judith M. A. van den Brand Dennis de Meulder Pascal Lexmond Theo M. Bestebroer Ron A. M. Fouchier
author_sort	Mathilde Richard
title	Mutations Driving Airborne Transmission of A/H5N1 Virus in Mammals Cause Substantial Attenuation in Chickens only when combined
title_short	Mutations Driving Airborne Transmission of A/H5N1 Virus in Mammals Cause Substantial Attenuation in Chickens only when combined
title_full	Mutations Driving Airborne Transmission of A/H5N1 Virus in Mammals Cause Substantial Attenuation in Chickens only when combined
title_fullStr	Mutations Driving Airborne Transmission of A/H5N1 Virus in Mammals Cause Substantial Attenuation in Chickens only when combined
title_full_unstemmed	Mutations Driving Airborne Transmission of A/H5N1 Virus in Mammals Cause Substantial Attenuation in Chickens only when combined
title_sort	mutations driving airborne transmission of a/h5n1 virus in mammals cause substantial attenuation in chickens only when combined
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/517fb06f055b4f4cae1e52f7a0491796
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Mutations Driving Airborne Transmission of A/H5N1 Virus in Mammals Cause Substantial Attenuation in Chickens only when combined

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