Random and combinatorial mutagenesis for improved total production of secretory target protein in Escherichia coli

Abstract Signal peptides and secretory carrier proteins are commonly used to secrete heterologous recombinant protein in Gram-negative bacteria. The Escherichia coli osmotically-inducible protein Y (OsmY) is a carrier protein that secretes a target protein extracellularly, and we have previously app...

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Autores principales: David Gonzalez-Perez, James Ratcliffe, Shu Khan Tan, Mary Chen May Wong, Yi Pei Yee, Natsai Nyabadza, Jian-He Xu, Tuck Seng Wong, Kang Lan Tee
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/51df7e5a175e403a9eddb2c4e1fddf6e
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