Random and combinatorial mutagenesis for improved total production of secretory target protein in Escherichia coli
Abstract Signal peptides and secretory carrier proteins are commonly used to secrete heterologous recombinant protein in Gram-negative bacteria. The Escherichia coli osmotically-inducible protein Y (OsmY) is a carrier protein that secretes a target protein extracellularly, and we have previously app...
Guardado en:
Autores principales: | David Gonzalez-Perez, James Ratcliffe, Shu Khan Tan, Mary Chen May Wong, Yi Pei Yee, Natsai Nyabadza, Jian-He Xu, Tuck Seng Wong, Kang Lan Tee |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2021
|
Materias: | |
Acceso en línea: | https://doaj.org/article/51df7e5a175e403a9eddb2c4e1fddf6e |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
Ejemplares similares
-
Comprehensive assessment of TP53 loss of function using multiple combinatorial mutagenesis libraries
por: Vincent Carbonnier, et al.
Publicado: (2020) -
Total biosynthesis of opiates by stepwise fermentation using engineered Escherichia coli
por: Akira Nakagawa, et al.
Publicado: (2016) -
A CRISPR-Cas9 based shuffle system for endogenous histone H3 and H4 combinatorial mutagenesis
por: Yu Fu, et al.
Publicado: (2021) -
Identification of an alpha-1 antitrypsin variant with enhanced specificity for factor XIa by phage display, bacterial expression, and combinatorial mutagenesis
por: Varsha Bhakta, et al.
Publicado: (2021) -
Engineered probiotic Escherichia coli can eliminate and prevent Pseudomonas aeruginosa gut infection in animal models
por: In Young Hwang, et al.
Publicado: (2017)