Mycobacterial Membrane Vesicles Administered Systemically in Mice Induce a Protective Immune Response to Surface Compartments of <named-content content-type="genus-species">Mycobacterium tuberculosis</named-content>

ABSTRACT Pathogenic and nonpathogenic species of bacteria and fungi release membrane vesicles (MV), containing proteins, polysaccharides, and lipids, into the extracellular milieu. Previously, we demonstrated that several mycobacterial species, including bacillus Calmette-Guerin (BCG) and Mycobacter...

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Autores principales:	Rafael Prados-Rosales, Leandro J. Carreño, Ana Batista-Gonzalez, Andres Baena, Manjunatha M. Venkataswamy, Jiayong Xu, Xiaobo Yu, Garrick Wallstrom, D. Mitchell Magee, Joshua LaBaer, Jacqueline M. Achkar, William R. Jacobs, John Chan, Steven A. Porcelli, Arturo Casadevall
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2014
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/521989cbc81a4c6ab47624c8ab6a1094
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spelling	oai:doaj.org-article:521989cbc81a4c6ab47624c8ab6a10942021-11-15T15:45:54ZMycobacterial Membrane Vesicles Administered Systemically in Mice Induce a Protective Immune Response to Surface Compartments of <named-content content-type="genus-species">Mycobacterium tuberculosis</named-content>10.1128/mBio.01921-142150-7511https://doaj.org/article/521989cbc81a4c6ab47624c8ab6a10942014-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01921-14https://doaj.org/toc/2150-7511ABSTRACT Pathogenic and nonpathogenic species of bacteria and fungi release membrane vesicles (MV), containing proteins, polysaccharides, and lipids, into the extracellular milieu. Previously, we demonstrated that several mycobacterial species, including bacillus Calmette-Guerin (BCG) and Mycobacterium tuberculosis, release MV containing lipids and proteins that subvert host immune response in a Toll-like receptor 2 (TLR2)-dependent manner (R. Prados-Rosales et al., J. Clin. Invest. 121:1471–1483, 2011, doi:10.1172/JCI44261). In this work, we analyzed the vaccine potential of MV in a mouse model and compared the effects of immunization with MV to those of standard BCG vaccination. Immunization with MV from BCG or M. tuberculosis elicited a mixed humoral and cellular response directed to both membrane and cell wall components, such as lipoproteins. However, only vaccination with M. tuberculosis MV was able to protect as well as live BCG immunization. M. tuberculosis MV boosted BCG vaccine efficacy. In summary, MV are highly immunogenic without adjuvants and elicit immune responses comparable to those achieved with BCG in protection against M. tuberculosis. IMPORTANCE This work offers a new vaccine approach against tuberculosis using mycobacterial MV. Mycobacterium MV are a naturally released product combining immunogenic antigens in the context of a lipid structure. The fact that MV do not need adjuvants and elicit protection comparable to that elicited by the BCG vaccine encourages vaccine approaches that combine protein antigens and lipids. Consequently, mycobacterium MV establish a new type of vaccine formulation.Rafael Prados-RosalesLeandro J. CarreñoAna Batista-GonzalezAndres BaenaManjunatha M. VenkataswamyJiayong XuXiaobo YuGarrick WallstromD. Mitchell MageeJoshua LaBaerJacqueline M. AchkarWilliam R. JacobsJohn ChanSteven A. PorcelliArturo CasadevallAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 5, Iss 5 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Rafael Prados-Rosales Leandro J. Carreño Ana Batista-Gonzalez Andres Baena Manjunatha M. Venkataswamy Jiayong Xu Xiaobo Yu Garrick Wallstrom D. Mitchell Magee Joshua LaBaer Jacqueline M. Achkar William R. Jacobs John Chan Steven A. Porcelli Arturo Casadevall Mycobacterial Membrane Vesicles Administered Systemically in Mice Induce a Protective Immune Response to Surface Compartments of <named-content content-type="genus-species">Mycobacterium tuberculosis</named-content>
description	ABSTRACT Pathogenic and nonpathogenic species of bacteria and fungi release membrane vesicles (MV), containing proteins, polysaccharides, and lipids, into the extracellular milieu. Previously, we demonstrated that several mycobacterial species, including bacillus Calmette-Guerin (BCG) and Mycobacterium tuberculosis, release MV containing lipids and proteins that subvert host immune response in a Toll-like receptor 2 (TLR2)-dependent manner (R. Prados-Rosales et al., J. Clin. Invest. 121:1471–1483, 2011, doi:10.1172/JCI44261). In this work, we analyzed the vaccine potential of MV in a mouse model and compared the effects of immunization with MV to those of standard BCG vaccination. Immunization with MV from BCG or M. tuberculosis elicited a mixed humoral and cellular response directed to both membrane and cell wall components, such as lipoproteins. However, only vaccination with M. tuberculosis MV was able to protect as well as live BCG immunization. M. tuberculosis MV boosted BCG vaccine efficacy. In summary, MV are highly immunogenic without adjuvants and elicit immune responses comparable to those achieved with BCG in protection against M. tuberculosis. IMPORTANCE This work offers a new vaccine approach against tuberculosis using mycobacterial MV. Mycobacterium MV are a naturally released product combining immunogenic antigens in the context of a lipid structure. The fact that MV do not need adjuvants and elicit protection comparable to that elicited by the BCG vaccine encourages vaccine approaches that combine protein antigens and lipids. Consequently, mycobacterium MV establish a new type of vaccine formulation.
format	article
author	Rafael Prados-Rosales Leandro J. Carreño Ana Batista-Gonzalez Andres Baena Manjunatha M. Venkataswamy Jiayong Xu Xiaobo Yu Garrick Wallstrom D. Mitchell Magee Joshua LaBaer Jacqueline M. Achkar William R. Jacobs John Chan Steven A. Porcelli Arturo Casadevall
author_facet	Rafael Prados-Rosales Leandro J. Carreño Ana Batista-Gonzalez Andres Baena Manjunatha M. Venkataswamy Jiayong Xu Xiaobo Yu Garrick Wallstrom D. Mitchell Magee Joshua LaBaer Jacqueline M. Achkar William R. Jacobs John Chan Steven A. Porcelli Arturo Casadevall
author_sort	Rafael Prados-Rosales
title	Mycobacterial Membrane Vesicles Administered Systemically in Mice Induce a Protective Immune Response to Surface Compartments of <named-content content-type="genus-species">Mycobacterium tuberculosis</named-content>
title_short	Mycobacterial Membrane Vesicles Administered Systemically in Mice Induce a Protective Immune Response to Surface Compartments of <named-content content-type="genus-species">Mycobacterium tuberculosis</named-content>
title_full	Mycobacterial Membrane Vesicles Administered Systemically in Mice Induce a Protective Immune Response to Surface Compartments of <named-content content-type="genus-species">Mycobacterium tuberculosis</named-content>
title_fullStr	Mycobacterial Membrane Vesicles Administered Systemically in Mice Induce a Protective Immune Response to Surface Compartments of <named-content content-type="genus-species">Mycobacterium tuberculosis</named-content>
title_full_unstemmed	Mycobacterial Membrane Vesicles Administered Systemically in Mice Induce a Protective Immune Response to Surface Compartments of <named-content content-type="genus-species">Mycobacterium tuberculosis</named-content>
title_sort	mycobacterial membrane vesicles administered systemically in mice induce a protective immune response to surface compartments of <named-content content-type="genus-species">mycobacterium tuberculosis</named-content>
publisher	American Society for Microbiology
publishDate	2014
url	https://doaj.org/article/521989cbc81a4c6ab47624c8ab6a1094
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Mycobacterial Membrane Vesicles Administered Systemically in Mice Induce a Protective Immune Response to Surface Compartments of <named-content content-type="genus-species">Mycobacterium tuberculosis</named-content>

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