Phage encoded H-NS: a potential achilles heel in the bacterial defence system.

The relationship between phage and their microbial hosts is difficult to elucidate in complex natural ecosystems. Engineered systems performing enhanced biological phosphorus removal (EBPR), offer stable, lower complexity communities for studying phage-host interactions. Here, metagenomic data from...

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Autores principales:	Connor T Skennerton, Florent E Angly, Mya Breitbart, Lauren Bragg, Shaomei He, Katherine D McMahon, Philip Hugenholtz, Gene W Tyson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5422968c68c946bfa34180d84c2450dc
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spelling	oai:doaj.org-article:5422968c68c946bfa34180d84c2450dc2021-11-18T06:53:46ZPhage encoded H-NS: a potential achilles heel in the bacterial defence system.1932-620310.1371/journal.pone.0020095https://doaj.org/article/5422968c68c946bfa34180d84c2450dc2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21625595/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The relationship between phage and their microbial hosts is difficult to elucidate in complex natural ecosystems. Engineered systems performing enhanced biological phosphorus removal (EBPR), offer stable, lower complexity communities for studying phage-host interactions. Here, metagenomic data from an EBPR reactor dominated by Candidatus Accumulibacter phosphatis (CAP), led to the recovery of three complete and six partial phage genomes. Heat-stable nucleoid structuring (H-NS) protein, a global transcriptional repressor in bacteria, was identified in one of the complete phage genomes (EPV1), and was most similar to a homolog in CAP. We infer that EPV1 is a CAP-specific phage and has the potential to repress up to 6% of host genes based on the presence of putative H-NS binding sites in the CAP genome. These genes include CRISPR associated proteins and a Type III restriction-modification system, which are key host defense mechanisms against phage infection. Further, EPV1 was the only member of the phage community found in an EBPR microbial metagenome collected seven months prior. We propose that EPV1 laterally acquired H-NS from CAP providing it with a means to reduce bacterial defenses, a selective advantage over other phage in the EBPR system. Phage encoded H-NS could constitute a previously unrecognized weapon in the phage-host arms race.Connor T SkennertonFlorent E AnglyMya BreitbartLauren BraggShaomei HeKatherine D McMahonPhilip HugenholtzGene W TysonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 5, p e20095 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Connor T Skennerton Florent E Angly Mya Breitbart Lauren Bragg Shaomei He Katherine D McMahon Philip Hugenholtz Gene W Tyson Phage encoded H-NS: a potential achilles heel in the bacterial defence system.
description	The relationship between phage and their microbial hosts is difficult to elucidate in complex natural ecosystems. Engineered systems performing enhanced biological phosphorus removal (EBPR), offer stable, lower complexity communities for studying phage-host interactions. Here, metagenomic data from an EBPR reactor dominated by Candidatus Accumulibacter phosphatis (CAP), led to the recovery of three complete and six partial phage genomes. Heat-stable nucleoid structuring (H-NS) protein, a global transcriptional repressor in bacteria, was identified in one of the complete phage genomes (EPV1), and was most similar to a homolog in CAP. We infer that EPV1 is a CAP-specific phage and has the potential to repress up to 6% of host genes based on the presence of putative H-NS binding sites in the CAP genome. These genes include CRISPR associated proteins and a Type III restriction-modification system, which are key host defense mechanisms against phage infection. Further, EPV1 was the only member of the phage community found in an EBPR microbial metagenome collected seven months prior. We propose that EPV1 laterally acquired H-NS from CAP providing it with a means to reduce bacterial defenses, a selective advantage over other phage in the EBPR system. Phage encoded H-NS could constitute a previously unrecognized weapon in the phage-host arms race.
format	article
author	Connor T Skennerton Florent E Angly Mya Breitbart Lauren Bragg Shaomei He Katherine D McMahon Philip Hugenholtz Gene W Tyson
author_facet	Connor T Skennerton Florent E Angly Mya Breitbart Lauren Bragg Shaomei He Katherine D McMahon Philip Hugenholtz Gene W Tyson
author_sort	Connor T Skennerton
title	Phage encoded H-NS: a potential achilles heel in the bacterial defence system.
title_short	Phage encoded H-NS: a potential achilles heel in the bacterial defence system.
title_full	Phage encoded H-NS: a potential achilles heel in the bacterial defence system.
title_fullStr	Phage encoded H-NS: a potential achilles heel in the bacterial defence system.
title_full_unstemmed	Phage encoded H-NS: a potential achilles heel in the bacterial defence system.
title_sort	phage encoded h-ns: a potential achilles heel in the bacterial defence system.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/5422968c68c946bfa34180d84c2450dc
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Phage encoded H-NS: a potential achilles heel in the bacterial defence system.

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