Assessment of gentamicin and cisplatin-induced kidney damage mediated via necrotic and apoptosis genes in albino rats

Abstract Background Gentamicin (GM) is a low-cost, low-resistance antibiotic commonly used to treat gram-negative bacterial diseases. Cisplatin (Csp) is a platinum-derived anti-neoplastic agent. This experiment aimed to identify the early signs of gentamicin and cisplatin-induced nephrotoxicity in r...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Tarek Kamal Abouzed, Eman Abd Elrahman Sherif, Mohamed El Sayed Barakat, Kadry Mohamed Sadek, Adil Aldhahrani, Nasr Elsayed Nasr, Ehab Eldomany, Khaled Khailo, Doaa Abdallha Dorghamm
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Gentamycin Cisplatin Nephrotoxicity TNFα Caspase 3 Bax Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/5423bcf856564e5485f8ae58f05c5092
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5423bcf856564e5485f8ae58f05c5092
record_format	dspace
spelling	oai:doaj.org-article:5423bcf856564e5485f8ae58f05c50922021-11-21T12:25:56ZAssessment of gentamicin and cisplatin-induced kidney damage mediated via necrotic and apoptosis genes in albino rats10.1186/s12917-021-03023-41746-6148https://doaj.org/article/5423bcf856564e5485f8ae58f05c50922021-11-01T00:00:00Zhttps://doi.org/10.1186/s12917-021-03023-4https://doaj.org/toc/1746-6148Abstract Background Gentamicin (GM) is a low-cost, low-resistance antibiotic commonly used to treat gram-negative bacterial diseases. Cisplatin (Csp) is a platinum-derived anti-neoplastic agent. This experiment aimed to identify the early signs of gentamicin and cisplatin-induced nephrotoxicity in rats. Thirty Wistar rats were divided into three groups of 10: a control group, which received no treatment; a gentamicin group administered by a dose of (100 mg/kg, IP) for 7 consecutive days, and a cisplatin group was administered intraperitoneal in a dose of (1.5 mg/kg body weight) repeated twice a week for 3 weeks. Results Both experimental groups exhibited increased levels of creatinine, urea, and uric acid, with the cisplatin-treated group showing higher levels than the gentamicin group. Experimental groups also exhibited significantly increased Malondialdehyde (MDA), reduced glutathione (GSH), and glutathione peroxidase (GSH-Px) with more pronounced effects in the cisplatin-treated group. Further, both experimental groups exhibited significant up-regulation of Tumor Necrosis Factor α (TNF-α), caspase-3, and Bax and down regulation of Bcl-2. Conclusion These findings confirm the use of necrotic, apoptotic genes as early biomarkers in the detection of tubular kidney damage. Further, cisplatin was shown to have a greater nephrotoxic effect than gentamicin; therefore, its use should be constrained accordingly when co-administered with gentamicin.Tarek Kamal AbouzedEman Abd Elrahman SherifMohamed El Sayed BarakatKadry Mohamed SadekAdil AldhahraniNasr Elsayed NasrEhab EldomanyKhaled KhailoDoaa Abdallha DorghammBMCarticleGentamycinCisplatinNephrotoxicityTNFαCaspase 3BaxVeterinary medicineSF600-1100ENBMC Veterinary Research, Vol 17, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Gentamycin Cisplatin Nephrotoxicity TNFα Caspase 3 Bax Veterinary medicine SF600-1100
spellingShingle	Gentamycin Cisplatin Nephrotoxicity TNFα Caspase 3 Bax Veterinary medicine SF600-1100 Tarek Kamal Abouzed Eman Abd Elrahman Sherif Mohamed El Sayed Barakat Kadry Mohamed Sadek Adil Aldhahrani Nasr Elsayed Nasr Ehab Eldomany Khaled Khailo Doaa Abdallha Dorghamm Assessment of gentamicin and cisplatin-induced kidney damage mediated via necrotic and apoptosis genes in albino rats
description	Abstract Background Gentamicin (GM) is a low-cost, low-resistance antibiotic commonly used to treat gram-negative bacterial diseases. Cisplatin (Csp) is a platinum-derived anti-neoplastic agent. This experiment aimed to identify the early signs of gentamicin and cisplatin-induced nephrotoxicity in rats. Thirty Wistar rats were divided into three groups of 10: a control group, which received no treatment; a gentamicin group administered by a dose of (100 mg/kg, IP) for 7 consecutive days, and a cisplatin group was administered intraperitoneal in a dose of (1.5 mg/kg body weight) repeated twice a week for 3 weeks. Results Both experimental groups exhibited increased levels of creatinine, urea, and uric acid, with the cisplatin-treated group showing higher levels than the gentamicin group. Experimental groups also exhibited significantly increased Malondialdehyde (MDA), reduced glutathione (GSH), and glutathione peroxidase (GSH-Px) with more pronounced effects in the cisplatin-treated group. Further, both experimental groups exhibited significant up-regulation of Tumor Necrosis Factor α (TNF-α), caspase-3, and Bax and down regulation of Bcl-2. Conclusion These findings confirm the use of necrotic, apoptotic genes as early biomarkers in the detection of tubular kidney damage. Further, cisplatin was shown to have a greater nephrotoxic effect than gentamicin; therefore, its use should be constrained accordingly when co-administered with gentamicin.
format	article
author	Tarek Kamal Abouzed Eman Abd Elrahman Sherif Mohamed El Sayed Barakat Kadry Mohamed Sadek Adil Aldhahrani Nasr Elsayed Nasr Ehab Eldomany Khaled Khailo Doaa Abdallha Dorghamm
author_facet	Tarek Kamal Abouzed Eman Abd Elrahman Sherif Mohamed El Sayed Barakat Kadry Mohamed Sadek Adil Aldhahrani Nasr Elsayed Nasr Ehab Eldomany Khaled Khailo Doaa Abdallha Dorghamm
author_sort	Tarek Kamal Abouzed
title	Assessment of gentamicin and cisplatin-induced kidney damage mediated via necrotic and apoptosis genes in albino rats
title_short	Assessment of gentamicin and cisplatin-induced kidney damage mediated via necrotic and apoptosis genes in albino rats
title_full	Assessment of gentamicin and cisplatin-induced kidney damage mediated via necrotic and apoptosis genes in albino rats
title_fullStr	Assessment of gentamicin and cisplatin-induced kidney damage mediated via necrotic and apoptosis genes in albino rats
title_full_unstemmed	Assessment of gentamicin and cisplatin-induced kidney damage mediated via necrotic and apoptosis genes in albino rats
title_sort	assessment of gentamicin and cisplatin-induced kidney damage mediated via necrotic and apoptosis genes in albino rats
publisher	BMC
publishDate	2021
url	https://doaj.org/article/5423bcf856564e5485f8ae58f05c5092
work_keys_str_mv	AT tarekkamalabouzed assessmentofgentamicinandcisplatininducedkidneydamagemediatedvianecroticandapoptosisgenesinalbinorats AT emanabdelrahmansherif assessmentofgentamicinandcisplatininducedkidneydamagemediatedvianecroticandapoptosisgenesinalbinorats AT mohamedelsayedbarakat assessmentofgentamicinandcisplatininducedkidneydamagemediatedvianecroticandapoptosisgenesinalbinorats AT kadrymohamedsadek assessmentofgentamicinandcisplatininducedkidneydamagemediatedvianecroticandapoptosisgenesinalbinorats AT adilaldhahrani assessmentofgentamicinandcisplatininducedkidneydamagemediatedvianecroticandapoptosisgenesinalbinorats AT nasrelsayednasr assessmentofgentamicinandcisplatininducedkidneydamagemediatedvianecroticandapoptosisgenesinalbinorats AT ehabeldomany assessmentofgentamicinandcisplatininducedkidneydamagemediatedvianecroticandapoptosisgenesinalbinorats AT khaledkhailo assessmentofgentamicinandcisplatininducedkidneydamagemediatedvianecroticandapoptosisgenesinalbinorats AT doaaabdallhadorghamm assessmentofgentamicinandcisplatininducedkidneydamagemediatedvianecroticandapoptosisgenesinalbinorats
_version_	1718419006977736704

Assessment of gentamicin and cisplatin-induced kidney damage mediated via necrotic and apoptosis genes in albino rats

Ejemplares similares