IL-4Rα-associated antigen processing by B cells promotes immunity in Nippostrongylus brasiliensis infection.

In this study, B cell function in protective T(H)2 immunity against N. brasiliensis infection was investigated. Protection against secondary infection depended on IL-4Rα and IL-13; but not IL-4. Protection did not associate with parasite specific antibody responses. Re-infection of B cell-specific I...

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Autores principales:	William G C Horsnell, Matthew G Darby, Jennifer C Hoving, Natalie Nieuwenhuizen, Henry J McSorley, Hlumani Ndlovu, Saeeda Bobat, Matti Kimberg, Frank Kirstein, Anthony J Cutler, Benjamin Dewals, Adam F Cunningham, Frank Brombacher
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/5476bf1fef48402eafedb4a555b17f51
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spelling	oai:doaj.org-article:5476bf1fef48402eafedb4a555b17f512021-11-18T06:07:26ZIL-4Rα-associated antigen processing by B cells promotes immunity in Nippostrongylus brasiliensis infection.1553-73661553-737410.1371/journal.ppat.1003662https://doaj.org/article/5476bf1fef48402eafedb4a555b17f512013-10-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24204255/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374In this study, B cell function in protective T(H)2 immunity against N. brasiliensis infection was investigated. Protection against secondary infection depended on IL-4Rα and IL-13; but not IL-4. Protection did not associate with parasite specific antibody responses. Re-infection of B cell-specific IL-4Rα⁻/⁻ mice resulted in increased worm burdens compared to control mice, despite their equivalent capacity to control primary infection. Impaired protection correlated with reduced lymphocyte IL-13 production and B cell MHC class II and CD86 surface expression. Adoptive transfer of in vivo N. brasiliensis primed IL-4Rα expressing B cells into naïve BALB/c mice, but not IL-4Rα or IL-13 deficient B cells, conferred protection against primary N. brasiliensis infection. This protection required MHC class II compatibility on B cells suggesting cognate interactions by B cells with CD4⁺ T cells were important to co-ordinate immunity. Furthermore, the rapid nature of these protective effects by B cells suggested non-BCR mediated mechanisms, such as via Toll Like Receptors, was involved, and this was supported by transfer experiments using antigen pulsed Myd88⁻/⁻ B cells. These data suggest TLR dependent antigen processing by IL-4Rα-responsive B cells producing IL-13 contribute significantly to CD4⁺ T cell-mediated protective immunity against N. brasiliensis infection.William G C HorsnellMatthew G DarbyJennifer C HovingNatalie NieuwenhuizenHenry J McSorleyHlumani NdlovuSaeeda BobatMatti KimbergFrank KirsteinAnthony J CutlerBenjamin DewalsAdam F CunninghamFrank BrombacherPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 9, Iss 10, p e1003662 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 William G C Horsnell Matthew G Darby Jennifer C Hoving Natalie Nieuwenhuizen Henry J McSorley Hlumani Ndlovu Saeeda Bobat Matti Kimberg Frank Kirstein Anthony J Cutler Benjamin Dewals Adam F Cunningham Frank Brombacher IL-4Rα-associated antigen processing by B cells promotes immunity in Nippostrongylus brasiliensis infection.
description	In this study, B cell function in protective T(H)2 immunity against N. brasiliensis infection was investigated. Protection against secondary infection depended on IL-4Rα and IL-13; but not IL-4. Protection did not associate with parasite specific antibody responses. Re-infection of B cell-specific IL-4Rα⁻/⁻ mice resulted in increased worm burdens compared to control mice, despite their equivalent capacity to control primary infection. Impaired protection correlated with reduced lymphocyte IL-13 production and B cell MHC class II and CD86 surface expression. Adoptive transfer of in vivo N. brasiliensis primed IL-4Rα expressing B cells into naïve BALB/c mice, but not IL-4Rα or IL-13 deficient B cells, conferred protection against primary N. brasiliensis infection. This protection required MHC class II compatibility on B cells suggesting cognate interactions by B cells with CD4⁺ T cells were important to co-ordinate immunity. Furthermore, the rapid nature of these protective effects by B cells suggested non-BCR mediated mechanisms, such as via Toll Like Receptors, was involved, and this was supported by transfer experiments using antigen pulsed Myd88⁻/⁻ B cells. These data suggest TLR dependent antigen processing by IL-4Rα-responsive B cells producing IL-13 contribute significantly to CD4⁺ T cell-mediated protective immunity against N. brasiliensis infection.
format	article
author	William G C Horsnell Matthew G Darby Jennifer C Hoving Natalie Nieuwenhuizen Henry J McSorley Hlumani Ndlovu Saeeda Bobat Matti Kimberg Frank Kirstein Anthony J Cutler Benjamin Dewals Adam F Cunningham Frank Brombacher
author_facet	William G C Horsnell Matthew G Darby Jennifer C Hoving Natalie Nieuwenhuizen Henry J McSorley Hlumani Ndlovu Saeeda Bobat Matti Kimberg Frank Kirstein Anthony J Cutler Benjamin Dewals Adam F Cunningham Frank Brombacher
author_sort	William G C Horsnell
title	IL-4Rα-associated antigen processing by B cells promotes immunity in Nippostrongylus brasiliensis infection.
title_short	IL-4Rα-associated antigen processing by B cells promotes immunity in Nippostrongylus brasiliensis infection.
title_full	IL-4Rα-associated antigen processing by B cells promotes immunity in Nippostrongylus brasiliensis infection.
title_fullStr	IL-4Rα-associated antigen processing by B cells promotes immunity in Nippostrongylus brasiliensis infection.
title_full_unstemmed	IL-4Rα-associated antigen processing by B cells promotes immunity in Nippostrongylus brasiliensis infection.
title_sort	il-4rα-associated antigen processing by b cells promotes immunity in nippostrongylus brasiliensis infection.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/5476bf1fef48402eafedb4a555b17f51
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IL-4Rα-associated antigen processing by B cells promotes immunity in Nippostrongylus brasiliensis infection.

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