D-Xylose Sensing in Saccharomyces cerevisiae: Insights from D-Glucose Signaling and Native D-Xylose Utilizers

Extension of the substrate range is among one of the metabolic engineering goals for microorganisms used in biotechnological processes because it enables the use of a wide range of raw materials as substrates. One of the most prominent examples is the engineering of baker’s yeast Saccharomy...

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Autores principales:	Daniel P. Brink, Celina Borgström, Viktor C. Persson, Karen Ofuji Osiro, Marie F. Gorwa-Grauslund
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Saccharomyces cerevisiae</i> <span style="font-variant: small-caps">d</span>-xylose sugar sensing sugar signaling non-native substrate signaling network engineering Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/5493b4f862084f3e93e0879fbac2d4a0
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spelling	oai:doaj.org-article:5493b4f862084f3e93e0879fbac2d4a02021-11-25T17:56:26Z<span style="font-variant: small-caps">D</span>-Xylose Sensing in <i>Saccharomyces cerevisiae</i>: Insights from <span style="font-variant: small-caps">D</span>-Glucose Signaling and Native <span style="font-variant: small-caps">D</span>-Xylose Utilizers10.3390/ijms2222124101422-00671661-6596https://doaj.org/article/5493b4f862084f3e93e0879fbac2d4a02021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12410https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Extension of the substrate range is among one of the metabolic engineering goals for microorganisms used in biotechnological processes because it enables the use of a wide range of raw materials as substrates. One of the most prominent examples is the engineering of baker’s yeast <i>Saccharomyces cerevisiae</i> for the utilization of <span style="font-variant: small-caps;">d-</span>xylose, a five-carbon sugar found in high abundance in lignocellulosic biomass and a key substrate to achieve good process economy in chemical production from renewable and non-edible plant feedstocks. Despite many excellent engineering strategies that have allowed recombinant <i>S. cerevisiae</i> to ferment <span style="font-variant: small-caps;">d-</span>xylose to ethanol at high yields, the consumption rate of <span style="font-variant: small-caps;">d</span>-xylose is still significantly lower than that of its preferred sugar <span style="font-variant: small-caps;">d</span>-glucose. In mixed <span style="font-variant: small-caps;">d</span>-glucose/<span style="font-variant: small-caps;">d</span>-xylose cultivations, <span style="font-variant: small-caps;">d</span>-xylose is only utilized after <span style="font-variant: small-caps;">d</span>-glucose depletion, which leads to prolonged process times and added costs. Due to this limitation, the response on <span style="font-variant: small-caps;">d</span>-xylose in the native sugar signaling pathways has emerged as a promising next-level engineering target. Here we review the current status of the knowledge of the response of <i>S. cerevisiae</i> signaling pathways to <span style="font-variant: small-caps;">d</span>-xylose. To do this, we first summarize the response of the native sensing and signaling pathways in <i>S. cerevisiae</i> to <span style="font-variant: small-caps;">d</span>-glucose (the preferred sugar of the yeast). Using the <span style="font-variant: small-caps;">d</span>-glucose case as a point of reference, we then proceed to discuss the known signaling response to <span style="font-variant: small-caps;">d</span>-xylose in <i>S. cerevisiae</i> and current attempts of improving the response by signaling engineering using native targets and synthetic (non-native) regulatory circuits.Daniel P. BrinkCelina BorgströmViktor C. PerssonKaren Ofuji OsiroMarie F. Gorwa-GrauslundMDPI AGarticle<i>Saccharomyces cerevisiae</i><span style="font-variant: small-caps">d</span>-xylosesugar sensingsugar signalingnon-native substratesignaling network engineeringBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12410, p 12410 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Saccharomyces cerevisiae</i> <span style="font-variant: small-caps">d</span>-xylose sugar sensing sugar signaling non-native substrate signaling network engineering Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	<i>Saccharomyces cerevisiae</i> <span style="font-variant: small-caps">d</span>-xylose sugar sensing sugar signaling non-native substrate signaling network engineering Biology (General) QH301-705.5 Chemistry QD1-999 Daniel P. Brink Celina Borgström Viktor C. Persson Karen Ofuji Osiro Marie F. Gorwa-Grauslund <span style="font-variant: small-caps">D</span>-Xylose Sensing in <i>Saccharomyces cerevisiae</i>: Insights from <span style="font-variant: small-caps">D</span>-Glucose Signaling and Native <span style="font-variant: small-caps">D</span>-Xylose Utilizers
description	Extension of the substrate range is among one of the metabolic engineering goals for microorganisms used in biotechnological processes because it enables the use of a wide range of raw materials as substrates. One of the most prominent examples is the engineering of baker’s yeast <i>Saccharomyces cerevisiae</i> for the utilization of <span style="font-variant: small-caps;">d-</span>xylose, a five-carbon sugar found in high abundance in lignocellulosic biomass and a key substrate to achieve good process economy in chemical production from renewable and non-edible plant feedstocks. Despite many excellent engineering strategies that have allowed recombinant <i>S. cerevisiae</i> to ferment <span style="font-variant: small-caps;">d-</span>xylose to ethanol at high yields, the consumption rate of <span style="font-variant: small-caps;">d</span>-xylose is still significantly lower than that of its preferred sugar <span style="font-variant: small-caps;">d</span>-glucose. In mixed <span style="font-variant: small-caps;">d</span>-glucose/<span style="font-variant: small-caps;">d</span>-xylose cultivations, <span style="font-variant: small-caps;">d</span>-xylose is only utilized after <span style="font-variant: small-caps;">d</span>-glucose depletion, which leads to prolonged process times and added costs. Due to this limitation, the response on <span style="font-variant: small-caps;">d</span>-xylose in the native sugar signaling pathways has emerged as a promising next-level engineering target. Here we review the current status of the knowledge of the response of <i>S. cerevisiae</i> signaling pathways to <span style="font-variant: small-caps;">d</span>-xylose. To do this, we first summarize the response of the native sensing and signaling pathways in <i>S. cerevisiae</i> to <span style="font-variant: small-caps;">d</span>-glucose (the preferred sugar of the yeast). Using the <span style="font-variant: small-caps;">d</span>-glucose case as a point of reference, we then proceed to discuss the known signaling response to <span style="font-variant: small-caps;">d</span>-xylose in <i>S. cerevisiae</i> and current attempts of improving the response by signaling engineering using native targets and synthetic (non-native) regulatory circuits.
format	article
author	Daniel P. Brink Celina Borgström Viktor C. Persson Karen Ofuji Osiro Marie F. Gorwa-Grauslund
author_facet	Daniel P. Brink Celina Borgström Viktor C. Persson Karen Ofuji Osiro Marie F. Gorwa-Grauslund
author_sort	Daniel P. Brink
title	<span style="font-variant: small-caps">D</span>-Xylose Sensing in <i>Saccharomyces cerevisiae</i>: Insights from <span style="font-variant: small-caps">D</span>-Glucose Signaling and Native <span style="font-variant: small-caps">D</span>-Xylose Utilizers
title_short	<span style="font-variant: small-caps">D</span>-Xylose Sensing in <i>Saccharomyces cerevisiae</i>: Insights from <span style="font-variant: small-caps">D</span>-Glucose Signaling and Native <span style="font-variant: small-caps">D</span>-Xylose Utilizers
title_full	<span style="font-variant: small-caps">D</span>-Xylose Sensing in <i>Saccharomyces cerevisiae</i>: Insights from <span style="font-variant: small-caps">D</span>-Glucose Signaling and Native <span style="font-variant: small-caps">D</span>-Xylose Utilizers
title_fullStr	<span style="font-variant: small-caps">D</span>-Xylose Sensing in <i>Saccharomyces cerevisiae</i>: Insights from <span style="font-variant: small-caps">D</span>-Glucose Signaling and Native <span style="font-variant: small-caps">D</span>-Xylose Utilizers
title_full_unstemmed	<span style="font-variant: small-caps">D</span>-Xylose Sensing in <i>Saccharomyces cerevisiae</i>: Insights from <span style="font-variant: small-caps">D</span>-Glucose Signaling and Native <span style="font-variant: small-caps">D</span>-Xylose Utilizers
title_sort	<span style="font-variant: small-caps">d</span>-xylose sensing in <i>saccharomyces cerevisiae</i>: insights from <span style="font-variant: small-caps">d</span>-glucose signaling and native <span style="font-variant: small-caps">d</span>-xylose utilizers
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/5493b4f862084f3e93e0879fbac2d4a0
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<span style="font-variant: small-caps">D</span>-Xylose Sensing in <i>Saccharomyces cerevisiae</i>: Insights from <span style="font-variant: small-caps">D</span>-Glucose Signaling and Native <span style="font-variant: small-caps">D</span>-Xylose Utilizers

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