Antimicrobial peptide-loaded liquid crystalline precursor bioadhesive system for the prevention of dental caries

Kelly Limi Aida,1 Paula Fernanda Kreling,1 Karina Sampaio Caiaffa,2 Giovana Maria Fioramonti Calixto,3 Marlus Chorilli,3 Denise MP Spolidorio,4 Norival Alves Santos-Filho,5,6 Eduardo Maffud Cilli,5 Cristiane Duque1 1Department of Pediatric Dentistry and Public Health, Araçatuba Dental Sc...

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Autores principales:	Aida KL, Kreling PF, Caiaffa KS, Calixto GMF, Chorilli M, Spolidorio DMP, Santos-Filho NA, Cilli EM, Duque C
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	peptide fragments drug delivery system dental caries biofilm Streptococcus mutans Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/564b95c8322944479661d491c9c9f3bd
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spelling	oai:doaj.org-article:564b95c8322944479661d491c9c9f3bd2021-12-02T02:24:21ZAntimicrobial peptide-loaded liquid crystalline precursor bioadhesive system for the prevention of dental caries1178-2013https://doaj.org/article/564b95c8322944479661d491c9c9f3bd2018-05-01T00:00:00Zhttps://www.dovepress.com/antimicrobial-peptide-loaded-liquid-crystalline-precursor-bioadhesive--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Kelly Limi Aida,1 Paula Fernanda Kreling,1 Karina Sampaio Caiaffa,2 Giovana Maria Fioramonti Calixto,3 Marlus Chorilli,3 Denise MP Spolidorio,4 Norival Alves Santos-Filho,5,6 Eduardo Maffud Cilli,5 Cristiane Duque1 1Department of Pediatric Dentistry and Public Health, Araçatuba Dental School, Sao Paulo State University (UNESP), Araçatuba, São Paulo, Brazil; 2Department of Endodontics, Araçatuba Dental School, Sao Paulo State University (UNESP), Araçatuba, São Paulo, Brazil; 3Department of Drugs and Medicines, School of Pharmaceutical Sciences, Sao Paulo State University (UNESP), Araraquara, São Paulo, Brazil; 4Department of Physiology and Pathology, Araraquara Dental School, Sao Paulo State University (UNESP), Araraquara, São Paulo, Brazil; 5Department of Biochemistry and Chemical Technology, Institute of Chemistry, Sao Paulo State University (UNESP), Araraquara, São Paulo, Brazil; 6Registro Experimental Campus, Sao Paulo State University (UNESP), Registro, São Paulo, Brazil Background: Anticaries agents must interfere with the adhesion of Streptococcus mutans and its proliferation in dental biofilm, without causing host toxicity and bacterial resistance. Natural substances, including cationic antimicrobial peptides (CAMPs) and their fragments, such as β-defensin-3 peptide fragment (D1–23), have been widely studied. However, the chemical and physical stability of CAMPs may be compromised by external factors, such as temperature and pH, reducing the period of antimicrobial activity. Methods: To overcome the aforementioned disadvantage, this study developed and characterized a drug delivery system and evaluated the cytotoxicity and effect against S. mutans biofilm of a D1–23-loaded bioadhesive liquid crystalline system (LCS). LCS was composed of oleic acid, polyoxypropylene-(5)-polyoxyethylene-(20)-cetyl alcohol, Carbopol® 974P and Carbopol® 971P. LCS was analyzed by polarized light microscopy (PLM), rheology (viscoelasticity and flow properties) and in vitro bioadhesion. The viability of epithelial cells was evaluated. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) against S. mutans were determined for D1–23 for further evaluation of the effect against S. mutans biofilm after 4 and 24 h of exposure to treatments. Results: PLM, rheology, and in vitro bioadhesion tests showed that both viscosity and bioadhesion of LCS increased after it was diluted with artificial saliva. D1–23-loaded LCS system presented better activity against S. mutans biofilm after 24 h when compared to 4 h of treatment, showing a cumulative effect. Neither LCS nor D1–23-loaded LCS presented toxicity on human epithelial cells. Conclusion: D1–23-loaded LCS is a promising drug delivery system for the prevention of dental caries. Keywords: peptide fragments, drug delivery system, dental caries, biofilm, Streptococcus mutansAida KLKreling PFCaiaffa KSCalixto GMFChorilli MSpolidorio DMPSantos-Filho NACilli EMDuque CDove Medical Pressarticlepeptide fragmentsdrug delivery systemdental cariesbiofilmStreptococcus mutansMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 3081-3091 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	peptide fragments drug delivery system dental caries biofilm Streptococcus mutans Medicine (General) R5-920
spellingShingle	peptide fragments drug delivery system dental caries biofilm Streptococcus mutans Medicine (General) R5-920 Aida KL Kreling PF Caiaffa KS Calixto GMF Chorilli M Spolidorio DMP Santos-Filho NA Cilli EM Duque C Antimicrobial peptide-loaded liquid crystalline precursor bioadhesive system for the prevention of dental caries
description	Kelly Limi Aida,1 Paula Fernanda Kreling,1 Karina Sampaio Caiaffa,2 Giovana Maria Fioramonti Calixto,3 Marlus Chorilli,3 Denise MP Spolidorio,4 Norival Alves Santos-Filho,5,6 Eduardo Maffud Cilli,5 Cristiane Duque1 1Department of Pediatric Dentistry and Public Health, Araçatuba Dental School, Sao Paulo State University (UNESP), Araçatuba, São Paulo, Brazil; 2Department of Endodontics, Araçatuba Dental School, Sao Paulo State University (UNESP), Araçatuba, São Paulo, Brazil; 3Department of Drugs and Medicines, School of Pharmaceutical Sciences, Sao Paulo State University (UNESP), Araraquara, São Paulo, Brazil; 4Department of Physiology and Pathology, Araraquara Dental School, Sao Paulo State University (UNESP), Araraquara, São Paulo, Brazil; 5Department of Biochemistry and Chemical Technology, Institute of Chemistry, Sao Paulo State University (UNESP), Araraquara, São Paulo, Brazil; 6Registro Experimental Campus, Sao Paulo State University (UNESP), Registro, São Paulo, Brazil Background: Anticaries agents must interfere with the adhesion of Streptococcus mutans and its proliferation in dental biofilm, without causing host toxicity and bacterial resistance. Natural substances, including cationic antimicrobial peptides (CAMPs) and their fragments, such as β-defensin-3 peptide fragment (D1–23), have been widely studied. However, the chemical and physical stability of CAMPs may be compromised by external factors, such as temperature and pH, reducing the period of antimicrobial activity. Methods: To overcome the aforementioned disadvantage, this study developed and characterized a drug delivery system and evaluated the cytotoxicity and effect against S. mutans biofilm of a D1–23-loaded bioadhesive liquid crystalline system (LCS). LCS was composed of oleic acid, polyoxypropylene-(5)-polyoxyethylene-(20)-cetyl alcohol, Carbopol® 974P and Carbopol® 971P. LCS was analyzed by polarized light microscopy (PLM), rheology (viscoelasticity and flow properties) and in vitro bioadhesion. The viability of epithelial cells was evaluated. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) against S. mutans were determined for D1–23 for further evaluation of the effect against S. mutans biofilm after 4 and 24 h of exposure to treatments. Results: PLM, rheology, and in vitro bioadhesion tests showed that both viscosity and bioadhesion of LCS increased after it was diluted with artificial saliva. D1–23-loaded LCS system presented better activity against S. mutans biofilm after 24 h when compared to 4 h of treatment, showing a cumulative effect. Neither LCS nor D1–23-loaded LCS presented toxicity on human epithelial cells. Conclusion: D1–23-loaded LCS is a promising drug delivery system for the prevention of dental caries. Keywords: peptide fragments, drug delivery system, dental caries, biofilm, Streptococcus mutans
format	article
author	Aida KL Kreling PF Caiaffa KS Calixto GMF Chorilli M Spolidorio DMP Santos-Filho NA Cilli EM Duque C
author_facet	Aida KL Kreling PF Caiaffa KS Calixto GMF Chorilli M Spolidorio DMP Santos-Filho NA Cilli EM Duque C
author_sort	Aida KL
title	Antimicrobial peptide-loaded liquid crystalline precursor bioadhesive system for the prevention of dental caries
title_short	Antimicrobial peptide-loaded liquid crystalline precursor bioadhesive system for the prevention of dental caries
title_full	Antimicrobial peptide-loaded liquid crystalline precursor bioadhesive system for the prevention of dental caries
title_fullStr	Antimicrobial peptide-loaded liquid crystalline precursor bioadhesive system for the prevention of dental caries
title_full_unstemmed	Antimicrobial peptide-loaded liquid crystalline precursor bioadhesive system for the prevention of dental caries
title_sort	antimicrobial peptide-loaded liquid crystalline precursor bioadhesive system for the prevention of dental caries
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/564b95c8322944479661d491c9c9f3bd
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Antimicrobial peptide-loaded liquid crystalline precursor bioadhesive system for the prevention of dental caries

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