An infinitely expandable cloning strategy plus repeat-proof PCR for working with multiple shRNA.

Vector construction with restriction enzymes (REs) typically involves the ligation of a digested donor fragment (insert) to a reciprocally digested recipient fragment (vector backbone). Creating a suitable cloning plan becomes increasingly difficult for complex strategies requiring repeated insertio...

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Autores principales: Glen John McIntyre, Jennifer Lynne Groneman, Anna Tran, Tanya Lynn Applegate
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2008
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Acceso en línea:https://doaj.org/article/5652a296c014455780fc3136aac76230
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