An infinitely expandable cloning strategy plus repeat-proof PCR for working with multiple shRNA.
Vector construction with restriction enzymes (REs) typically involves the ligation of a digested donor fragment (insert) to a reciprocally digested recipient fragment (vector backbone). Creating a suitable cloning plan becomes increasingly difficult for complex strategies requiring repeated insertio...
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Autores principales: | , , , |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Public Library of Science (PLoS)
2008
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Materias: | |
Acceso en línea: | https://doaj.org/article/5652a296c014455780fc3136aac76230 |
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