Semi-automated closed system manufacturing of lentivirus gene-modified haematopoietic stem cells for gene therapy

Current methods for haematopoietic stem cell gene therapy are laborious and require special licensed facilities. Here the authors develop a semi-automated protocol using a commercially available device to allow for benchtop generation of gene-modified blood cell products for transplantation, that me...

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Autores principales: Jennifer E. Adair, Timothy Waters, Kevin G. Haworth, Sara P. Kubek, Grant D. Trobridge, Jonah D. Hocum, Shelly Heimfeld, Hans-Peter Kiem
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2016
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Acceso en línea:https://doaj.org/article/583cfd673dbc436f8e0a56f2549b1b65
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Sumario:Current methods for haematopoietic stem cell gene therapy are laborious and require special licensed facilities. Here the authors develop a semi-automated protocol using a commercially available device to allow for benchtop generation of gene-modified blood cell products for transplantation, that meet current standards.