Aldosterone Modulates the Association between NCC and ENaC

Abstract Distal sodium transport is a final step in the regulation of blood pressure. As such, understanding how the two main sodium transport proteins, the thiazide-sensitive sodium chloride cotransporter (NCC) and the epithelial sodium channel (ENaC), are regulated is paramount. Both are expressed...

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Autores principales:	Brandi M. Wynne, Abinash C. Mistry, Otor Al-Khalili, Rickta Mallick, Franziska Theilig, Douglas C. Eaton, Robert S. Hoover
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5a57c393beb9449b97dd8ad6093995d4
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spelling	oai:doaj.org-article:5a57c393beb9449b97dd8ad6093995d42021-12-02T15:05:16ZAldosterone Modulates the Association between NCC and ENaC10.1038/s41598-017-03510-52045-2322https://doaj.org/article/5a57c393beb9449b97dd8ad6093995d42017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03510-5https://doaj.org/toc/2045-2322Abstract Distal sodium transport is a final step in the regulation of blood pressure. As such, understanding how the two main sodium transport proteins, the thiazide-sensitive sodium chloride cotransporter (NCC) and the epithelial sodium channel (ENaC), are regulated is paramount. Both are expressed in the late distal nephron; however, no evidence has suggested that these two sodium transport proteins interact. Recently, we established that these two sodium transport proteins functionally interact in the second part of the distal nephron (DCT2). Given their co-localization within the DCT2, we hypothesized that NCC and ENaC interactions might be modulated by aldosterone (Aldo). Aldo treatment increased NCC and αENaC colocalization (electron microscopy) and interaction (coimmunoprecipitation). Finally, with co-expression of the Aldo-induced protein serum- and glucocorticoid-inducible kinase 1 (SGK1), NCC and αENaC interactions were increased. These data demonstrate that Aldo promotes increased interaction of NCC and ENaC, within the DCT2 revealing a novel method of regulation for distal sodium reabsorption.Brandi M. WynneAbinash C. MistryOtor Al-KhaliliRickta MallickFranziska TheiligDouglas C. EatonRobert S. HooverNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Brandi M. Wynne Abinash C. Mistry Otor Al-Khalili Rickta Mallick Franziska Theilig Douglas C. Eaton Robert S. Hoover Aldosterone Modulates the Association between NCC and ENaC
description	Abstract Distal sodium transport is a final step in the regulation of blood pressure. As such, understanding how the two main sodium transport proteins, the thiazide-sensitive sodium chloride cotransporter (NCC) and the epithelial sodium channel (ENaC), are regulated is paramount. Both are expressed in the late distal nephron; however, no evidence has suggested that these two sodium transport proteins interact. Recently, we established that these two sodium transport proteins functionally interact in the second part of the distal nephron (DCT2). Given their co-localization within the DCT2, we hypothesized that NCC and ENaC interactions might be modulated by aldosterone (Aldo). Aldo treatment increased NCC and αENaC colocalization (electron microscopy) and interaction (coimmunoprecipitation). Finally, with co-expression of the Aldo-induced protein serum- and glucocorticoid-inducible kinase 1 (SGK1), NCC and αENaC interactions were increased. These data demonstrate that Aldo promotes increased interaction of NCC and ENaC, within the DCT2 revealing a novel method of regulation for distal sodium reabsorption.
format	article
author	Brandi M. Wynne Abinash C. Mistry Otor Al-Khalili Rickta Mallick Franziska Theilig Douglas C. Eaton Robert S. Hoover
author_facet	Brandi M. Wynne Abinash C. Mistry Otor Al-Khalili Rickta Mallick Franziska Theilig Douglas C. Eaton Robert S. Hoover
author_sort	Brandi M. Wynne
title	Aldosterone Modulates the Association between NCC and ENaC
title_short	Aldosterone Modulates the Association between NCC and ENaC
title_full	Aldosterone Modulates the Association between NCC and ENaC
title_fullStr	Aldosterone Modulates the Association between NCC and ENaC
title_full_unstemmed	Aldosterone Modulates the Association between NCC and ENaC
title_sort	aldosterone modulates the association between ncc and enac
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5a57c393beb9449b97dd8ad6093995d4
work_keys_str_mv	AT brandimwynne aldosteronemodulatestheassociationbetweennccandenac AT abinashcmistry aldosteronemodulatestheassociationbetweennccandenac AT otoralkhalili aldosteronemodulatestheassociationbetweennccandenac AT ricktamallick aldosteronemodulatestheassociationbetweennccandenac AT franziskatheilig aldosteronemodulatestheassociationbetweennccandenac AT douglasceaton aldosteronemodulatestheassociationbetweennccandenac AT robertshoover aldosteronemodulatestheassociationbetweennccandenac
_version_	1718388890982678528

Aldosterone Modulates the Association between NCC and ENaC

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