A proteomics protocol to identify stimulation-induced binding partners dependent on a specific gene in mammalian cells
Summary: Some protein-protein interactions are induced by different kinds of stimulation and are dependent on specific genes. To identify these interaction partners, we present a protocol which utilizes affinity purification of Flag-tagged protein complexes followed by mass-spectrometry-based proteo...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | article |
| Language: | EN |
| Published: |
Elsevier
2021
|
| Subjects: | |
| Online Access: | https://doaj.org/article/5b067d16d3214e3b9d2f7d55f45809db |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Be the first to leave a comment!