A quantitative sequencing framework for absolute abundance measurements of mucosal and lumenal microbial communities

Changes in microbial taxa are commonly derived from estimations of relative abundance, which inherently limits the depth of the analysis. Here, the authors present an absolute quantification method based on digital PCR anchoring and 16S rRNA gene sequencing and show how a ketogenic diet affects indi...

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Autores principales: Jacob T. Barlow, Said R. Bogatyrev, Rustem F. Ismagilov
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/5b905800d963464b804135207031a781
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