A quantitative sequencing framework for absolute abundance measurements of mucosal and lumenal microbial communities

Changes in microbial taxa are commonly derived from estimations of relative abundance, which inherently limits the depth of the analysis. Here, the authors present an absolute quantification method based on digital PCR anchoring and 16S rRNA gene sequencing and show how a ketogenic diet affects indi...

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Autores principales:	Jacob T. Barlow, Said R. Bogatyrev, Rustem F. Ismagilov
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/5b905800d963464b804135207031a781
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id	oai:doaj.org-article:5b905800d963464b804135207031a781
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spelling	oai:doaj.org-article:5b905800d963464b804135207031a7812021-12-02T16:51:25ZA quantitative sequencing framework for absolute abundance measurements of mucosal and lumenal microbial communities10.1038/s41467-020-16224-62041-1723https://doaj.org/article/5b905800d963464b804135207031a7812020-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-16224-6https://doaj.org/toc/2041-1723Changes in microbial taxa are commonly derived from estimations of relative abundance, which inherently limits the depth of the analysis. Here, the authors present an absolute quantification method based on digital PCR anchoring and 16S rRNA gene sequencing and show how a ketogenic diet affects individual taxon’s absolute abundance in stool and small-intestine mucosa samples in miceJacob T. BarlowSaid R. BogatyrevRustem F. IsmagilovNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jacob T. Barlow Said R. Bogatyrev Rustem F. Ismagilov A quantitative sequencing framework for absolute abundance measurements of mucosal and lumenal microbial communities
description	Changes in microbial taxa are commonly derived from estimations of relative abundance, which inherently limits the depth of the analysis. Here, the authors present an absolute quantification method based on digital PCR anchoring and 16S rRNA gene sequencing and show how a ketogenic diet affects individual taxon’s absolute abundance in stool and small-intestine mucosa samples in mice
format	article
author	Jacob T. Barlow Said R. Bogatyrev Rustem F. Ismagilov
author_facet	Jacob T. Barlow Said R. Bogatyrev Rustem F. Ismagilov
author_sort	Jacob T. Barlow
title	A quantitative sequencing framework for absolute abundance measurements of mucosal and lumenal microbial communities
title_short	A quantitative sequencing framework for absolute abundance measurements of mucosal and lumenal microbial communities
title_full	A quantitative sequencing framework for absolute abundance measurements of mucosal and lumenal microbial communities
title_fullStr	A quantitative sequencing framework for absolute abundance measurements of mucosal and lumenal microbial communities
title_full_unstemmed	A quantitative sequencing framework for absolute abundance measurements of mucosal and lumenal microbial communities
title_sort	quantitative sequencing framework for absolute abundance measurements of mucosal and lumenal microbial communities
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/5b905800d963464b804135207031a781
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A quantitative sequencing framework for absolute abundance measurements of mucosal and lumenal microbial communities

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