A quantitative sequencing framework for absolute abundance measurements of mucosal and lumenal microbial communities
Changes in microbial taxa are commonly derived from estimations of relative abundance, which inherently limits the depth of the analysis. Here, the authors present an absolute quantification method based on digital PCR anchoring and 16S rRNA gene sequencing and show how a ketogenic diet affects indi...
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Autores principales: | Jacob T. Barlow, Said R. Bogatyrev, Rustem F. Ismagilov |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2020
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Materias: | |
Acceso en línea: | https://doaj.org/article/5b905800d963464b804135207031a781 |
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