A bladder cancer patient-derived xenograft displays aggressive growth dynamics in vivo and in organoid culture

Abstract Bladder cancer is among the most prevalent cancers worldwide. Currently, few bladder cancer models have undergone thorough characterization to assess their fidelity to patient tumors, especially upon propagation in the laboratory. Here, we establish and molecularly characterize CoCaB 1, an...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Elise Y. Cai, Jose Garcia, Yuzhen Liu, Funda Vakar-Lopez, Sonali Arora, Holly M. Nguyen, Bryce Lakely, Lisha Brown, Alicia Wong, Bruce Montgomery, John K. Lee, Eva Corey, Jonathan L. Wright, Andrew C. Hsieh, Hung-Ming Lam
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5b9f2e7e7f544668b01d526be6457f1a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5b9f2e7e7f544668b01d526be6457f1a
record_format	dspace
spelling	oai:doaj.org-article:5b9f2e7e7f544668b01d526be6457f1a2021-12-02T13:34:57ZA bladder cancer patient-derived xenograft displays aggressive growth dynamics in vivo and in organoid culture10.1038/s41598-021-83662-72045-2322https://doaj.org/article/5b9f2e7e7f544668b01d526be6457f1a2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83662-7https://doaj.org/toc/2045-2322Abstract Bladder cancer is among the most prevalent cancers worldwide. Currently, few bladder cancer models have undergone thorough characterization to assess their fidelity to patient tumors, especially upon propagation in the laboratory. Here, we establish and molecularly characterize CoCaB 1, an aggressive cisplatin-resistant muscle-invasive bladder cancer patient-derived xenograft (PDX) and companion organoid system. CoCaB 1 was a subcutaneous PDX model reliably transplanted in vivo and demonstrated an acceleration in growth upon serial transplantation, which was reflected in organoid and 2D cell culture systems. Transcriptome analysis revealed progression towards an increasingly proliferative and stem-like expression profile. Gene expression differences between organoid and PDX models reflected expected differences in cellular composition, with organoids enriched in lipid biosynthesis and metabolism genes and deprived of extracellular components observed in PDXs. Both PDX and organoid models maintained the histological fidelity and mutational heterogeneity of their parental tumor. This study establishes the CoCaB 1 PDX and organoid system as companion representative tumor models for the development of novel bladder cancer therapies.Elise Y. CaiJose GarciaYuzhen LiuFunda Vakar-LopezSonali AroraHolly M. NguyenBryce LakelyLisha BrownAlicia WongBruce MontgomeryJohn K. LeeEva CoreyJonathan L. WrightAndrew C. HsiehHung-Ming LamNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elise Y. Cai Jose Garcia Yuzhen Liu Funda Vakar-Lopez Sonali Arora Holly M. Nguyen Bryce Lakely Lisha Brown Alicia Wong Bruce Montgomery John K. Lee Eva Corey Jonathan L. Wright Andrew C. Hsieh Hung-Ming Lam A bladder cancer patient-derived xenograft displays aggressive growth dynamics in vivo and in organoid culture
description	Abstract Bladder cancer is among the most prevalent cancers worldwide. Currently, few bladder cancer models have undergone thorough characterization to assess their fidelity to patient tumors, especially upon propagation in the laboratory. Here, we establish and molecularly characterize CoCaB 1, an aggressive cisplatin-resistant muscle-invasive bladder cancer patient-derived xenograft (PDX) and companion organoid system. CoCaB 1 was a subcutaneous PDX model reliably transplanted in vivo and demonstrated an acceleration in growth upon serial transplantation, which was reflected in organoid and 2D cell culture systems. Transcriptome analysis revealed progression towards an increasingly proliferative and stem-like expression profile. Gene expression differences between organoid and PDX models reflected expected differences in cellular composition, with organoids enriched in lipid biosynthesis and metabolism genes and deprived of extracellular components observed in PDXs. Both PDX and organoid models maintained the histological fidelity and mutational heterogeneity of their parental tumor. This study establishes the CoCaB 1 PDX and organoid system as companion representative tumor models for the development of novel bladder cancer therapies.
format	article
author	Elise Y. Cai Jose Garcia Yuzhen Liu Funda Vakar-Lopez Sonali Arora Holly M. Nguyen Bryce Lakely Lisha Brown Alicia Wong Bruce Montgomery John K. Lee Eva Corey Jonathan L. Wright Andrew C. Hsieh Hung-Ming Lam
author_facet	Elise Y. Cai Jose Garcia Yuzhen Liu Funda Vakar-Lopez Sonali Arora Holly M. Nguyen Bryce Lakely Lisha Brown Alicia Wong Bruce Montgomery John K. Lee Eva Corey Jonathan L. Wright Andrew C. Hsieh Hung-Ming Lam
author_sort	Elise Y. Cai
title	A bladder cancer patient-derived xenograft displays aggressive growth dynamics in vivo and in organoid culture
title_short	A bladder cancer patient-derived xenograft displays aggressive growth dynamics in vivo and in organoid culture
title_full	A bladder cancer patient-derived xenograft displays aggressive growth dynamics in vivo and in organoid culture
title_fullStr	A bladder cancer patient-derived xenograft displays aggressive growth dynamics in vivo and in organoid culture
title_full_unstemmed	A bladder cancer patient-derived xenograft displays aggressive growth dynamics in vivo and in organoid culture
title_sort	bladder cancer patient-derived xenograft displays aggressive growth dynamics in vivo and in organoid culture
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5b9f2e7e7f544668b01d526be6457f1a
work_keys_str_mv	AT eliseycai abladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT josegarcia abladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT yuzhenliu abladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT fundavakarlopez abladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT sonaliarora abladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT hollymnguyen abladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT brycelakely abladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT lishabrown abladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT aliciawong abladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT brucemontgomery abladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT johnklee abladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT evacorey abladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT jonathanlwright abladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT andrewchsieh abladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT hungminglam abladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT eliseycai bladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT josegarcia bladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT yuzhenliu bladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT fundavakarlopez bladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT sonaliarora bladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT hollymnguyen bladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT brycelakely bladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT lishabrown bladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT aliciawong bladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT brucemontgomery bladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT johnklee bladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT evacorey bladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT jonathanlwright bladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT andrewchsieh bladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture AT hungminglam bladdercancerpatientderivedxenograftdisplaysaggressivegrowthdynamicsinvivoandinorganoidculture
_version_	1718392737536933888

A bladder cancer patient-derived xenograft displays aggressive growth dynamics in vivo and in organoid culture

Ejemplares similares