Cellular and molecular characterization of multipolar Map5-expressing cells: a subset of newly generated, stage-specific parenchymal cells in the mammalian central nervous system.

Although extremely interesting in adult neuro-glio-genesis and promising as an endogenous source for repair, parenchymal progenitors remain largely obscure in their identity and physiology, due to a scarce availability of stage-specific markers. What appears difficult is the distinction between real...

Description complète

Enregistré dans:

Détails bibliographiques
Auteurs principaux:	Paola Crociara, Roberta Parolisi, Daniele Conte, Marta Fumagalli, Luca Bonfanti
Format:	article
Langue:	EN
Publié:	Public Library of Science (PLoS) 2013
Sujets:	Medicine R Science Q
Accès en ligne:	https://doaj.org/article/5c306cf726bf4cada38af2c7e25aeed5
Tags:	Ajouter un tag Pas de tags, Soyez le premier à ajouter un tag!

id	oai:doaj.org-article:5c306cf726bf4cada38af2c7e25aeed5
record_format	dspace
spelling	oai:doaj.org-article:5c306cf726bf4cada38af2c7e25aeed52021-11-18T07:46:34ZCellular and molecular characterization of multipolar Map5-expressing cells: a subset of newly generated, stage-specific parenchymal cells in the mammalian central nervous system.1932-620310.1371/journal.pone.0063258https://doaj.org/article/5c306cf726bf4cada38af2c7e25aeed52013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23667595/?tool=EBIhttps://doaj.org/toc/1932-6203Although extremely interesting in adult neuro-glio-genesis and promising as an endogenous source for repair, parenchymal progenitors remain largely obscure in their identity and physiology, due to a scarce availability of stage-specific markers. What appears difficult is the distinction between real cell populations and various differentiation stages of the same population. Here we focused on a subset of multipolar, polydendrocyte-like cells (mMap5 cells) expressing the microtubule associated protein 5 (Map5), which is known to be present in most neurons. We characterized the morphology, phenotype, regional distribution, proliferative dynamics, and stage-specific marker expression of these cells in the rabbit and mouse CNS, also assessing their existence in other mammalian species. mMap5 cells were never found to co-express the Ng2 antigen. They appear to be a population of glial cells sharing features but also differences with Ng2+progenitor cells. We show that mMap5 cells are newly generated, postmitotic parenchymal elements of the oligodendroglial lineage, thus being a stage-specific population of polydendrocytes. Finally, we report that the number of mMap5 cells, although reduced within the brain of adult/old animals, can increase in neurodegenerative and traumatic conditions.Paola CrociaraRoberta ParolisiDaniele ConteMarta FumagalliLuca BonfantiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e63258 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Paola Crociara Roberta Parolisi Daniele Conte Marta Fumagalli Luca Bonfanti Cellular and molecular characterization of multipolar Map5-expressing cells: a subset of newly generated, stage-specific parenchymal cells in the mammalian central nervous system.
description	Although extremely interesting in adult neuro-glio-genesis and promising as an endogenous source for repair, parenchymal progenitors remain largely obscure in their identity and physiology, due to a scarce availability of stage-specific markers. What appears difficult is the distinction between real cell populations and various differentiation stages of the same population. Here we focused on a subset of multipolar, polydendrocyte-like cells (mMap5 cells) expressing the microtubule associated protein 5 (Map5), which is known to be present in most neurons. We characterized the morphology, phenotype, regional distribution, proliferative dynamics, and stage-specific marker expression of these cells in the rabbit and mouse CNS, also assessing their existence in other mammalian species. mMap5 cells were never found to co-express the Ng2 antigen. They appear to be a population of glial cells sharing features but also differences with Ng2+progenitor cells. We show that mMap5 cells are newly generated, postmitotic parenchymal elements of the oligodendroglial lineage, thus being a stage-specific population of polydendrocytes. Finally, we report that the number of mMap5 cells, although reduced within the brain of adult/old animals, can increase in neurodegenerative and traumatic conditions.
format	article
author	Paola Crociara Roberta Parolisi Daniele Conte Marta Fumagalli Luca Bonfanti
author_facet	Paola Crociara Roberta Parolisi Daniele Conte Marta Fumagalli Luca Bonfanti
author_sort	Paola Crociara
title	Cellular and molecular characterization of multipolar Map5-expressing cells: a subset of newly generated, stage-specific parenchymal cells in the mammalian central nervous system.
title_short	Cellular and molecular characterization of multipolar Map5-expressing cells: a subset of newly generated, stage-specific parenchymal cells in the mammalian central nervous system.
title_full	Cellular and molecular characterization of multipolar Map5-expressing cells: a subset of newly generated, stage-specific parenchymal cells in the mammalian central nervous system.
title_fullStr	Cellular and molecular characterization of multipolar Map5-expressing cells: a subset of newly generated, stage-specific parenchymal cells in the mammalian central nervous system.
title_full_unstemmed	Cellular and molecular characterization of multipolar Map5-expressing cells: a subset of newly generated, stage-specific parenchymal cells in the mammalian central nervous system.
title_sort	cellular and molecular characterization of multipolar map5-expressing cells: a subset of newly generated, stage-specific parenchymal cells in the mammalian central nervous system.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/5c306cf726bf4cada38af2c7e25aeed5
work_keys_str_mv	AT paolacrociara cellularandmolecularcharacterizationofmultipolarmap5expressingcellsasubsetofnewlygeneratedstagespecificparenchymalcellsinthemammaliancentralnervoussystem AT robertaparolisi cellularandmolecularcharacterizationofmultipolarmap5expressingcellsasubsetofnewlygeneratedstagespecificparenchymalcellsinthemammaliancentralnervoussystem AT danieleconte cellularandmolecularcharacterizationofmultipolarmap5expressingcellsasubsetofnewlygeneratedstagespecificparenchymalcellsinthemammaliancentralnervoussystem AT martafumagalli cellularandmolecularcharacterizationofmultipolarmap5expressingcellsasubsetofnewlygeneratedstagespecificparenchymalcellsinthemammaliancentralnervoussystem AT lucabonfanti cellularandmolecularcharacterizationofmultipolarmap5expressingcellsasubsetofnewlygeneratedstagespecificparenchymalcellsinthemammaliancentralnervoussystem
_version_	1718422973025615872

Cellular and molecular characterization of multipolar Map5-expressing cells: a subset of newly generated, stage-specific parenchymal cells in the mammalian central nervous system.

Documents similaires