Local administration of HMGB-1 promotes bone regeneration on the critical-sized mandibular defects in rabbits

Abstract Reconstruction of a critical-sized osseous defect is challenging in maxillofacial surgery. Despite novel treatments and advances in supportive therapies, severe complications including infection, nonunion, and malunion can still occur. Here, we aimed to assess the use of a beta-tricalcium p...

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Autores principales:	Ahmed Monir, Taro Mukaibo, Abdel Basit M. Abd El-Aal, Tomotaka Nodai, Takashi Munemasa, Yusuke Kondo, Chihiro Masaki, Mahasen A. El-Shair, Kou Matsuo, Ryuji Hosokawa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5cd80a41744f43059bf72386a771eb72
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spelling	oai:doaj.org-article:5cd80a41744f43059bf72386a771eb722021-12-02T13:40:51ZLocal administration of HMGB-1 promotes bone regeneration on the critical-sized mandibular defects in rabbits10.1038/s41598-021-88195-72045-2322https://doaj.org/article/5cd80a41744f43059bf72386a771eb722021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88195-7https://doaj.org/toc/2045-2322Abstract Reconstruction of a critical-sized osseous defect is challenging in maxillofacial surgery. Despite novel treatments and advances in supportive therapies, severe complications including infection, nonunion, and malunion can still occur. Here, we aimed to assess the use of a beta-tricalcium phosphate (β-TCP) scaffold loaded with high mobility group box-1 protein (HMGB-1) as a novel critical-sized bone defect treatment in rabbits. The study was performed on 15 specific pathogen-free New Zealand rabbits divided into three groups: Group A had an osseous defect filled with a β-TCP scaffold loaded with phosphate-buffered saline (PBS) (100 µL/scaffold), the defect in group B was filled with recombinant human bone morphogenetic protein 2 (rhBMP-2) (10 µg/100 µL), and the defect in group C was loaded with HMGB-1 (10 µg/100 µL). Micro-computed tomography (CT) examination demonstrated that group C (HMGB-1) showed the highest new bone volume ratio, with a mean value of 66.5%, followed by the group B (rhBMP-2) (31.0%), and group A (Control) (7.1%). Histological examination of the HMGB-1 treated group showed a vast area covered by lamellar and woven bone surrounding the β-TCP granule remnants. These results suggest that HMGB-1 could be an effective alternative molecule for bone regeneration in critical-sized mandibular bone defects.Ahmed MonirTaro MukaiboAbdel Basit M. Abd El-AalTomotaka NodaiTakashi MunemasaYusuke KondoChihiro MasakiMahasen A. El-ShairKou MatsuoRyuji HosokawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ahmed Monir Taro Mukaibo Abdel Basit M. Abd El-Aal Tomotaka Nodai Takashi Munemasa Yusuke Kondo Chihiro Masaki Mahasen A. El-Shair Kou Matsuo Ryuji Hosokawa Local administration of HMGB-1 promotes bone regeneration on the critical-sized mandibular defects in rabbits
description	Abstract Reconstruction of a critical-sized osseous defect is challenging in maxillofacial surgery. Despite novel treatments and advances in supportive therapies, severe complications including infection, nonunion, and malunion can still occur. Here, we aimed to assess the use of a beta-tricalcium phosphate (β-TCP) scaffold loaded with high mobility group box-1 protein (HMGB-1) as a novel critical-sized bone defect treatment in rabbits. The study was performed on 15 specific pathogen-free New Zealand rabbits divided into three groups: Group A had an osseous defect filled with a β-TCP scaffold loaded with phosphate-buffered saline (PBS) (100 µL/scaffold), the defect in group B was filled with recombinant human bone morphogenetic protein 2 (rhBMP-2) (10 µg/100 µL), and the defect in group C was loaded with HMGB-1 (10 µg/100 µL). Micro-computed tomography (CT) examination demonstrated that group C (HMGB-1) showed the highest new bone volume ratio, with a mean value of 66.5%, followed by the group B (rhBMP-2) (31.0%), and group A (Control) (7.1%). Histological examination of the HMGB-1 treated group showed a vast area covered by lamellar and woven bone surrounding the β-TCP granule remnants. These results suggest that HMGB-1 could be an effective alternative molecule for bone regeneration in critical-sized mandibular bone defects.
format	article
author	Ahmed Monir Taro Mukaibo Abdel Basit M. Abd El-Aal Tomotaka Nodai Takashi Munemasa Yusuke Kondo Chihiro Masaki Mahasen A. El-Shair Kou Matsuo Ryuji Hosokawa
author_facet	Ahmed Monir Taro Mukaibo Abdel Basit M. Abd El-Aal Tomotaka Nodai Takashi Munemasa Yusuke Kondo Chihiro Masaki Mahasen A. El-Shair Kou Matsuo Ryuji Hosokawa
author_sort	Ahmed Monir
title	Local administration of HMGB-1 promotes bone regeneration on the critical-sized mandibular defects in rabbits
title_short	Local administration of HMGB-1 promotes bone regeneration on the critical-sized mandibular defects in rabbits
title_full	Local administration of HMGB-1 promotes bone regeneration on the critical-sized mandibular defects in rabbits
title_fullStr	Local administration of HMGB-1 promotes bone regeneration on the critical-sized mandibular defects in rabbits
title_full_unstemmed	Local administration of HMGB-1 promotes bone regeneration on the critical-sized mandibular defects in rabbits
title_sort	local administration of hmgb-1 promotes bone regeneration on the critical-sized mandibular defects in rabbits
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5cd80a41744f43059bf72386a771eb72
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Local administration of HMGB-1 promotes bone regeneration on the critical-sized mandibular defects in rabbits

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