Functional memory B cells and long-lived plasma cells are generated after a single Plasmodium chabaudi infection in mice.

Antibodies have long been shown to play a critical role in naturally acquired immunity to malaria, but it has been suggested that Plasmodium-specific antibodies in humans may not be long lived. The cellular mechanisms underlying B cell and antibody responses are difficult to study in human infection...

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Autores principales:	Francis Maina Ndungu, Emma Tamsin Cadman, Joshua Coulcher, Eunice Nduati, Elisabeth Couper, Douglas William Macdonald, Dorothy Ng, Jean Langhorne
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/5ce6b9585b2547c6a6346b8af8a95232
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spelling	oai:doaj.org-article:5ce6b9585b2547c6a6346b8af8a952322021-11-25T05:48:27ZFunctional memory B cells and long-lived plasma cells are generated after a single Plasmodium chabaudi infection in mice.1553-73661553-737410.1371/journal.ppat.1000690https://doaj.org/article/5ce6b9585b2547c6a6346b8af8a952322009-12-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20011127/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Antibodies have long been shown to play a critical role in naturally acquired immunity to malaria, but it has been suggested that Plasmodium-specific antibodies in humans may not be long lived. The cellular mechanisms underlying B cell and antibody responses are difficult to study in human infections; therefore, we have investigated the kinetics, duration and characteristics of the Plasmodium-specific memory B cell response in an infection of P. chabaudi in mice. Memory B cells and plasma cells specific for the C-terminal region of Merozoite Surface Protein 1 were detectable for more than eight months following primary infection. Furthermore, a classical memory response comprised predominantly of the T-cell dependent isotypes IgG2c, IgG2b and IgG1 was elicited upon rechallenge with the homologous parasite, confirming the generation of functional memory B cells. Using cyclophosphamide treatment to discriminate between long-lived and short-lived plasma cells, we demonstrated long-lived cells secreting Plasmodium-specific IgG in both bone marrow and in spleens of infected mice. The presence of these long-lived cells was independent of the presence of chronic infection, as removal of parasites with anti-malarial drugs had no impact on their numbers. Thus, in this model of malaria, both functional Plasmodium-specific memory B cells and long-lived plasma cells can be generated, suggesting that defects in generating these cell populations may not be the reason for generating short-lived antibody responses.Francis Maina NdunguEmma Tamsin CadmanJoshua CoulcherEunice NduatiElisabeth CouperDouglas William MacdonaldDorothy NgJean LanghornePublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 5, Iss 12, p e1000690 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Francis Maina Ndungu Emma Tamsin Cadman Joshua Coulcher Eunice Nduati Elisabeth Couper Douglas William Macdonald Dorothy Ng Jean Langhorne Functional memory B cells and long-lived plasma cells are generated after a single Plasmodium chabaudi infection in mice.
description	Antibodies have long been shown to play a critical role in naturally acquired immunity to malaria, but it has been suggested that Plasmodium-specific antibodies in humans may not be long lived. The cellular mechanisms underlying B cell and antibody responses are difficult to study in human infections; therefore, we have investigated the kinetics, duration and characteristics of the Plasmodium-specific memory B cell response in an infection of P. chabaudi in mice. Memory B cells and plasma cells specific for the C-terminal region of Merozoite Surface Protein 1 were detectable for more than eight months following primary infection. Furthermore, a classical memory response comprised predominantly of the T-cell dependent isotypes IgG2c, IgG2b and IgG1 was elicited upon rechallenge with the homologous parasite, confirming the generation of functional memory B cells. Using cyclophosphamide treatment to discriminate between long-lived and short-lived plasma cells, we demonstrated long-lived cells secreting Plasmodium-specific IgG in both bone marrow and in spleens of infected mice. The presence of these long-lived cells was independent of the presence of chronic infection, as removal of parasites with anti-malarial drugs had no impact on their numbers. Thus, in this model of malaria, both functional Plasmodium-specific memory B cells and long-lived plasma cells can be generated, suggesting that defects in generating these cell populations may not be the reason for generating short-lived antibody responses.
format	article
author	Francis Maina Ndungu Emma Tamsin Cadman Joshua Coulcher Eunice Nduati Elisabeth Couper Douglas William Macdonald Dorothy Ng Jean Langhorne
author_facet	Francis Maina Ndungu Emma Tamsin Cadman Joshua Coulcher Eunice Nduati Elisabeth Couper Douglas William Macdonald Dorothy Ng Jean Langhorne
author_sort	Francis Maina Ndungu
title	Functional memory B cells and long-lived plasma cells are generated after a single Plasmodium chabaudi infection in mice.
title_short	Functional memory B cells and long-lived plasma cells are generated after a single Plasmodium chabaudi infection in mice.
title_full	Functional memory B cells and long-lived plasma cells are generated after a single Plasmodium chabaudi infection in mice.
title_fullStr	Functional memory B cells and long-lived plasma cells are generated after a single Plasmodium chabaudi infection in mice.
title_full_unstemmed	Functional memory B cells and long-lived plasma cells are generated after a single Plasmodium chabaudi infection in mice.
title_sort	functional memory b cells and long-lived plasma cells are generated after a single plasmodium chabaudi infection in mice.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/5ce6b9585b2547c6a6346b8af8a95232
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Functional memory B cells and long-lived plasma cells are generated after a single Plasmodium chabaudi infection in mice.

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