An intragenic mutagenesis strategy in Physcomitrella patens to preserve intron splicing

Abstract Gene targeting is a powerful reverse genetics technique for site-specific genome modification. Intrinsic homologous recombination in the moss Physcomitrella patens permits highly effective gene targeting, a characteristic that makes this organism a valuable model for functional genetics. Fu...

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Auteurs principaux: Ako Eugene Ako, Pierre-François Perroud, Joseph Innocent, Viktor Demko, Odd-Arne Olsen, Wenche Johansen
Format: article
Langue:EN
Publié: Nature Portfolio 2017
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Accès en ligne:https://doaj.org/article/5d791c27f43f41b2865c04cd62b2a9bd
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