Structural basis for the promiscuous PAM recognition by Corynebacterium diphtheriae Cas9

The RNA-guided DNA endonuclease Cas9 from Corynebacterium diphtheriae (CdCas9) recognizes a promiscuous protospacer adjacent motif (PAM). Here the authors provide insights into the CdCas9-mediated PAM recognition mechanism by determining the 2.9 Å crystal structure of CdCas9 in complex with the guid...

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Autores principales:	Seiichi Hirano, Omar O. Abudayyeh, Jonathan S. Gootenberg, Takuro Horii, Ryuichiro Ishitani, Izuho Hatada, Feng Zhang, Hiroshi Nishimasu, Osamu Nureki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/5de5b028ca60413797f77392310eac3b
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spelling	oai:doaj.org-article:5de5b028ca60413797f77392310eac3b2021-12-02T15:35:11ZStructural basis for the promiscuous PAM recognition by Corynebacterium diphtheriae Cas910.1038/s41467-019-09741-62041-1723https://doaj.org/article/5de5b028ca60413797f77392310eac3b2019-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-09741-6https://doaj.org/toc/2041-1723The RNA-guided DNA endonuclease Cas9 from Corynebacterium diphtheriae (CdCas9) recognizes a promiscuous protospacer adjacent motif (PAM). Here the authors provide insights into the CdCas9-mediated PAM recognition mechanism by determining the 2.9 Å crystal structure of CdCas9 in complex with the guide RNA and its target DNA, which is of interest for engineering of CRISPR-Cas9 genome-editor nucleases.Seiichi HiranoOmar O. AbudayyehJonathan S. GootenbergTakuro HoriiRyuichiro IshitaniIzuho HatadaFeng ZhangHiroshi NishimasuOsamu NurekiNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Seiichi Hirano Omar O. Abudayyeh Jonathan S. Gootenberg Takuro Horii Ryuichiro Ishitani Izuho Hatada Feng Zhang Hiroshi Nishimasu Osamu Nureki Structural basis for the promiscuous PAM recognition by Corynebacterium diphtheriae Cas9
description	The RNA-guided DNA endonuclease Cas9 from Corynebacterium diphtheriae (CdCas9) recognizes a promiscuous protospacer adjacent motif (PAM). Here the authors provide insights into the CdCas9-mediated PAM recognition mechanism by determining the 2.9 Å crystal structure of CdCas9 in complex with the guide RNA and its target DNA, which is of interest for engineering of CRISPR-Cas9 genome-editor nucleases.
format	article
author	Seiichi Hirano Omar O. Abudayyeh Jonathan S. Gootenberg Takuro Horii Ryuichiro Ishitani Izuho Hatada Feng Zhang Hiroshi Nishimasu Osamu Nureki
author_facet	Seiichi Hirano Omar O. Abudayyeh Jonathan S. Gootenberg Takuro Horii Ryuichiro Ishitani Izuho Hatada Feng Zhang Hiroshi Nishimasu Osamu Nureki
author_sort	Seiichi Hirano
title	Structural basis for the promiscuous PAM recognition by Corynebacterium diphtheriae Cas9
title_short	Structural basis for the promiscuous PAM recognition by Corynebacterium diphtheriae Cas9
title_full	Structural basis for the promiscuous PAM recognition by Corynebacterium diphtheriae Cas9
title_fullStr	Structural basis for the promiscuous PAM recognition by Corynebacterium diphtheriae Cas9
title_full_unstemmed	Structural basis for the promiscuous PAM recognition by Corynebacterium diphtheriae Cas9
title_sort	structural basis for the promiscuous pam recognition by corynebacterium diphtheriae cas9
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/5de5b028ca60413797f77392310eac3b
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Structural basis for the promiscuous PAM recognition by Corynebacterium diphtheriae Cas9

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