Removing auto-activators from yeast-two-hybrid assays by conditional negative selection

Abstract Yeast-two-hybrid (Y2H) is widely used as a strategy to detect protein–protein interactions (PPIs). Recent advancements have made it possible to generate and analyse genome-wide PPI networks en masse by coupling Y2H with next-generation sequencing technology. However, one of the major challe...

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Autores principales: Devendra Shivhare, Magdalena Musialak-Lange, Irene Julca, Pawel Gluza, Marek Mutwil
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/5e8525fb58be461fad6a353f30568090
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