Detection of membrane-bound proteases of Francisella tularensis

Sterile preparations of membrane fractions were prepared by processing of live Francisella tularensis cells of different subspecies with 4.5 M urea solution and differential centrifugation. For the first time, proteolytic activity was detected and studied by tests of radial enzyme diffusion and subs...

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Main Authors: A. V. Korneva, V. B. Nikolaev, C. N. Kozlov, E. Y. Markov, A. V. Mazepa, Y. O. Popova
Format: article
Language:RU
Published: Scientific Сentre for Family Health and Human Reproduction Problems 2016
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Online Access:https://doaj.org/article/5e8d87e3dc604e499c1ef952406c676b
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