Detection of membrane-bound proteases of Francisella tularensis

Sterile preparations of membrane fractions were prepared by processing of live Francisella tularensis cells of different subspecies with 4.5 M urea solution and differential centrifugation. For the first time, proteolytic activity was detected and studied by tests of radial enzyme diffusion and subs...

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Autores principales:	A. V. Korneva, V. B. Nikolaev, C. N. Kozlov, E. Y. Markov, A. V. Mazepa, Y. O. Popova
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2016
Materias:	francisella tularensis subcellular fractions outer membrane enzyme protease proteolytic activity Science Q
Acceso en línea:	https://doaj.org/article/5e8d87e3dc604e499c1ef952406c676b
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spelling	oai:doaj.org-article:5e8d87e3dc604e499c1ef952406c676b2021-11-23T06:14:36ZDetection of membrane-bound proteases of Francisella tularensis2541-94202587-959610.12737/23416https://doaj.org/article/5e8d87e3dc604e499c1ef952406c676b2016-09-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/300https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Sterile preparations of membrane fractions were prepared by processing of live Francisella tularensis cells of different subspecies with 4.5 M urea solution and differential centrifugation. For the first time, proteolytic activity was detected and studied by tests of radial enzyme diffusion and substrate polyacrylamide gel electrophoresis using gelatin as a substrate. Spectrum of gelatinases in the resulted preparation were detected. Quantitative inter-strain differences in the protease activities and their qualitative composition in membrane preparations of various virulent F. tularensis strains was analyzed. Avirulent F. tularensis 21/400 subsp. holarctica (1-214) strain demonstrated the greatest gelatinase activity in enzyme diffusion method and the lowest hydrolytic activity was seen in F. tularensis B-399 A-Cole subsp. tularensis (1-386) and F. tularensis Utah 112 subsp. novicida (1-384), other preparations showed intermediate activity. Enzyme electrophoresis in the protease spectra determined the presence of proteins with proteases activity 50-100 kDa, and in the spectrum preparations of F. tularensis 1-386 and 1-384 were detected additional bands of proteases.A. V. KornevaV. B. NikolaevC. N. KozlovE. Y. MarkovA. V. MazepaY. O. PopovaScientific Сentre for Family Health and Human Reproduction Problemsarticlefrancisella tularensisfrancisella tularensissubcellular fractionsouter membraneenzymeproteaseproteolytic activityScienceQRUActa Biomedica Scientifica, Vol 1, Iss 5, Pp 155-159 (2016)
institution	DOAJ
collection	DOAJ
language	RU
topic	francisella tularensis francisella tularensis subcellular fractions outer membrane enzyme protease proteolytic activity Science Q
spellingShingle	francisella tularensis francisella tularensis subcellular fractions outer membrane enzyme protease proteolytic activity Science Q A. V. Korneva V. B. Nikolaev C. N. Kozlov E. Y. Markov A. V. Mazepa Y. O. Popova Detection of membrane-bound proteases of Francisella tularensis
description	Sterile preparations of membrane fractions were prepared by processing of live Francisella tularensis cells of different subspecies with 4.5 M urea solution and differential centrifugation. For the first time, proteolytic activity was detected and studied by tests of radial enzyme diffusion and substrate polyacrylamide gel electrophoresis using gelatin as a substrate. Spectrum of gelatinases in the resulted preparation were detected. Quantitative inter-strain differences in the protease activities and their qualitative composition in membrane preparations of various virulent F. tularensis strains was analyzed. Avirulent F. tularensis 21/400 subsp. holarctica (1-214) strain demonstrated the greatest gelatinase activity in enzyme diffusion method and the lowest hydrolytic activity was seen in F. tularensis B-399 A-Cole subsp. tularensis (1-386) and F. tularensis Utah 112 subsp. novicida (1-384), other preparations showed intermediate activity. Enzyme electrophoresis in the protease spectra determined the presence of proteins with proteases activity 50-100 kDa, and in the spectrum preparations of F. tularensis 1-386 and 1-384 were detected additional bands of proteases.
format	article
author	A. V. Korneva V. B. Nikolaev C. N. Kozlov E. Y. Markov A. V. Mazepa Y. O. Popova
author_facet	A. V. Korneva V. B. Nikolaev C. N. Kozlov E. Y. Markov A. V. Mazepa Y. O. Popova
author_sort	A. V. Korneva
title	Detection of membrane-bound proteases of Francisella tularensis
title_short	Detection of membrane-bound proteases of Francisella tularensis
title_full	Detection of membrane-bound proteases of Francisella tularensis
title_fullStr	Detection of membrane-bound proteases of Francisella tularensis
title_full_unstemmed	Detection of membrane-bound proteases of Francisella tularensis
title_sort	detection of membrane-bound proteases of francisella tularensis
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2016
url	https://doaj.org/article/5e8d87e3dc604e499c1ef952406c676b
work_keys_str_mv	AT avkorneva detectionofmembraneboundproteasesoffrancisellatularensis AT vbnikolaev detectionofmembraneboundproteasesoffrancisellatularensis AT cnkozlov detectionofmembraneboundproteasesoffrancisellatularensis AT eymarkov detectionofmembraneboundproteasesoffrancisellatularensis AT avmazepa detectionofmembraneboundproteasesoffrancisellatularensis AT yopopova detectionofmembraneboundproteasesoffrancisellatularensis
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Detection of membrane-bound proteases of Francisella tularensis

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