A Novel 3D Culture Model of Human ASCs Reduces Cell Death in Spheroid Cores and Maintains Inner Cell Proliferation Compared With a Nonadherent 3D Culture

3D cell culture technologies have recently shown very valuable promise for applications in regenerative medicine, but the most common 3D culture methods for mesenchymal stem cells still have limitations for clinical application, mainly due to the slowdown of inner cell proliferation and increase in...

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Autores principales:	Liang Luo, Wei Zhang, Jing Wang, Ming Zhao, Kuo Shen, Yanhui Jia, Yan Li, Jian Zhang, Weixia Cai, Dan Xiao, Xiaozhi Bai, Kaituo Liu, Kejia Wang, Yue Zhang, Huayu Zhu, Qin Zhou, Dahai Hu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	3D culture organoid ASCs 3D-ASC transdifferentiation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/5f1fbbda193b40ffaf2ffd2533ccb64f
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spelling	oai:doaj.org-article:5f1fbbda193b40ffaf2ffd2533ccb64f2021-11-11T09:55:43ZA Novel 3D Culture Model of Human ASCs Reduces Cell Death in Spheroid Cores and Maintains Inner Cell Proliferation Compared With a Nonadherent 3D Culture2296-634X10.3389/fcell.2021.737275https://doaj.org/article/5f1fbbda193b40ffaf2ffd2533ccb64f2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.737275/fullhttps://doaj.org/toc/2296-634X3D cell culture technologies have recently shown very valuable promise for applications in regenerative medicine, but the most common 3D culture methods for mesenchymal stem cells still have limitations for clinical application, mainly due to the slowdown of inner cell proliferation and increase in cell death rate. We previously developed a new 3D culture of adipose-derived mesenchymal stem cells (ASCs) based on its self-feeder layer, which solves the two issues of ASC 3D cell culture on ultra-low attachment (ULA) surface. In this study, we compared the 3D spheroids formed on the self-feeder layer (SLF-3D ASCs) with the spheroids formed by using ULA plates (ULA-3D ASCs). We discovered that the cells of SLF-3D spheroids still have a greater proliferation ability than ULA-3D ASCs, and the volume of these spheroids increases rather than shrinks, with more viable cells in 3D spheroids compared with the ULA-3D ASCs. Furthermore, it was discovered that the SLF-3D ASCs are likely to exhibit the abovementioned unique properties due to change in the expression level of ECM-related genes, like COL3A1, MMP3, HAS1, and FN1. These results indicate that the SLF-3D spheroid is a promising way forward for clinical application.Liang LuoWei ZhangJing WangMing ZhaoKuo ShenYanhui JiaYan LiJian ZhangWeixia CaiDan XiaoXiaozhi BaiKaituo LiuKejia WangYue ZhangHuayu ZhuQin ZhouDahai HuFrontiers Media S.A.article3D cultureorganoidASCs3D-ASCtransdifferentiationBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	3D culture organoid ASCs 3D-ASC transdifferentiation Biology (General) QH301-705.5
spellingShingle	3D culture organoid ASCs 3D-ASC transdifferentiation Biology (General) QH301-705.5 Liang Luo Wei Zhang Jing Wang Ming Zhao Kuo Shen Yanhui Jia Yan Li Jian Zhang Weixia Cai Dan Xiao Xiaozhi Bai Kaituo Liu Kejia Wang Yue Zhang Huayu Zhu Qin Zhou Dahai Hu A Novel 3D Culture Model of Human ASCs Reduces Cell Death in Spheroid Cores and Maintains Inner Cell Proliferation Compared With a Nonadherent 3D Culture
description	3D cell culture technologies have recently shown very valuable promise for applications in regenerative medicine, but the most common 3D culture methods for mesenchymal stem cells still have limitations for clinical application, mainly due to the slowdown of inner cell proliferation and increase in cell death rate. We previously developed a new 3D culture of adipose-derived mesenchymal stem cells (ASCs) based on its self-feeder layer, which solves the two issues of ASC 3D cell culture on ultra-low attachment (ULA) surface. In this study, we compared the 3D spheroids formed on the self-feeder layer (SLF-3D ASCs) with the spheroids formed by using ULA plates (ULA-3D ASCs). We discovered that the cells of SLF-3D spheroids still have a greater proliferation ability than ULA-3D ASCs, and the volume of these spheroids increases rather than shrinks, with more viable cells in 3D spheroids compared with the ULA-3D ASCs. Furthermore, it was discovered that the SLF-3D ASCs are likely to exhibit the abovementioned unique properties due to change in the expression level of ECM-related genes, like COL3A1, MMP3, HAS1, and FN1. These results indicate that the SLF-3D spheroid is a promising way forward for clinical application.
format	article
author	Liang Luo Wei Zhang Jing Wang Ming Zhao Kuo Shen Yanhui Jia Yan Li Jian Zhang Weixia Cai Dan Xiao Xiaozhi Bai Kaituo Liu Kejia Wang Yue Zhang Huayu Zhu Qin Zhou Dahai Hu
author_facet	Liang Luo Wei Zhang Jing Wang Ming Zhao Kuo Shen Yanhui Jia Yan Li Jian Zhang Weixia Cai Dan Xiao Xiaozhi Bai Kaituo Liu Kejia Wang Yue Zhang Huayu Zhu Qin Zhou Dahai Hu
author_sort	Liang Luo
title	A Novel 3D Culture Model of Human ASCs Reduces Cell Death in Spheroid Cores and Maintains Inner Cell Proliferation Compared With a Nonadherent 3D Culture
title_short	A Novel 3D Culture Model of Human ASCs Reduces Cell Death in Spheroid Cores and Maintains Inner Cell Proliferation Compared With a Nonadherent 3D Culture
title_full	A Novel 3D Culture Model of Human ASCs Reduces Cell Death in Spheroid Cores and Maintains Inner Cell Proliferation Compared With a Nonadherent 3D Culture
title_fullStr	A Novel 3D Culture Model of Human ASCs Reduces Cell Death in Spheroid Cores and Maintains Inner Cell Proliferation Compared With a Nonadherent 3D Culture
title_full_unstemmed	A Novel 3D Culture Model of Human ASCs Reduces Cell Death in Spheroid Cores and Maintains Inner Cell Proliferation Compared With a Nonadherent 3D Culture
title_sort	novel 3d culture model of human ascs reduces cell death in spheroid cores and maintains inner cell proliferation compared with a nonadherent 3d culture
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/5f1fbbda193b40ffaf2ffd2533ccb64f
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A Novel 3D Culture Model of Human ASCs Reduces Cell Death in Spheroid Cores and Maintains Inner Cell Proliferation Compared With a Nonadherent 3D Culture

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