The DNA co-vaccination using Sm antigen and IL-10 as prophylactic experimental therapy ameliorates nephritis in a model of lupus induced by pristane.

<h4>Introduction</h4>Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by the production of autoantibodies such as anti-Sm. Studies in patients with SLE and murine models of lupus reveal that the most critical anti-Sm autoantibodies are predominantly direct agains...

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Autores principales:	Beatriz Teresita Martín-Márquez, Minoru Satoh, Rogelio Hernández-Pando, Erika Aurora Martínez-García, Marcelo Heron Petri, Flavio Sandoval-García, Oscar Pizano-Martinez, Trinidad García-Iglesias, Fernanda Isadora Corona-Meraz, Monica Vázquez-Del Mercado
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5f69cb4eb2bf4954bb583b30ad84d7bc
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spelling	oai:doaj.org-article:5f69cb4eb2bf4954bb583b30ad84d7bc2021-12-02T20:13:27ZThe DNA co-vaccination using Sm antigen and IL-10 as prophylactic experimental therapy ameliorates nephritis in a model of lupus induced by pristane.1932-620310.1371/journal.pone.0259114https://doaj.org/article/5f69cb4eb2bf4954bb583b30ad84d7bc2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0259114https://doaj.org/toc/1932-6203<h4>Introduction</h4>Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by the production of autoantibodies such as anti-Sm. Studies in patients with SLE and murine models of lupus reveal that the most critical anti-Sm autoantibodies are predominantly direct against D1(83-119), D2, and B´/B epitopes.<h4>Objectives</h4>The present study aimed to analyze the induction of antigen-specific tolerance after prophylactic immunization with a DNA vaccine encoding the epitopes: D183-119, D2, B´/B, and B´/BCOOH in co-vaccination with IFN-γ or IL-10 in a murine model of lupus induced by pristane.<h4>Material and methods</h4>To obtain endotoxin-free DNA vaccines, direct cloning techniques using pcDNA were performed: D183-119, D2, B´/B, B´/BCOOH, IFN-γ, or IL-10. Lupus was induced by 0.5 mL of pristane via intraperitoneal in BALB/c female mice. Immunoprecipitation with K562 cells was metabolically labeled with 35S and ELISA to detect serum antibodies or mice IgG1, IgG2a isotypes. ELISA determined IL-10 and IFN-γ from splenocytes supernatants. Proteinuria was assessed monthly, and lupus nephritis was evaluated by immunofluorescence, and electron microscopy.<h4>Results</h4>The prophylactic co-vaccination with D2/IL-10 reduced the expression of kidney damage observed by electron microscopy, direct immunofluorescence, and H & E, along with reduced level of anti-nRNP/Sm antibodies (P = 0.048).<h4>Conclusion</h4>The prophylactic co-vaccination of IL-10 with D2 in pristane-induced lupus ameliorates the renal damage maybe by acting as prophylactic DNA tolerizing therapy.Beatriz Teresita Martín-MárquezMinoru SatohRogelio Hernández-PandoErika Aurora Martínez-GarcíaMarcelo Heron PetriFlavio Sandoval-GarcíaOscar Pizano-MartinezTrinidad García-IglesiasFernanda Isadora Corona-MerazMonica Vázquez-Del MercadoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0259114 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Beatriz Teresita Martín-Márquez Minoru Satoh Rogelio Hernández-Pando Erika Aurora Martínez-García Marcelo Heron Petri Flavio Sandoval-García Oscar Pizano-Martinez Trinidad García-Iglesias Fernanda Isadora Corona-Meraz Monica Vázquez-Del Mercado The DNA co-vaccination using Sm antigen and IL-10 as prophylactic experimental therapy ameliorates nephritis in a model of lupus induced by pristane.
description	<h4>Introduction</h4>Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by the production of autoantibodies such as anti-Sm. Studies in patients with SLE and murine models of lupus reveal that the most critical anti-Sm autoantibodies are predominantly direct against D1(83-119), D2, and B´/B epitopes.<h4>Objectives</h4>The present study aimed to analyze the induction of antigen-specific tolerance after prophylactic immunization with a DNA vaccine encoding the epitopes: D183-119, D2, B´/B, and B´/BCOOH in co-vaccination with IFN-γ or IL-10 in a murine model of lupus induced by pristane.<h4>Material and methods</h4>To obtain endotoxin-free DNA vaccines, direct cloning techniques using pcDNA were performed: D183-119, D2, B´/B, B´/BCOOH, IFN-γ, or IL-10. Lupus was induced by 0.5 mL of pristane via intraperitoneal in BALB/c female mice. Immunoprecipitation with K562 cells was metabolically labeled with 35S and ELISA to detect serum antibodies or mice IgG1, IgG2a isotypes. ELISA determined IL-10 and IFN-γ from splenocytes supernatants. Proteinuria was assessed monthly, and lupus nephritis was evaluated by immunofluorescence, and electron microscopy.<h4>Results</h4>The prophylactic co-vaccination with D2/IL-10 reduced the expression of kidney damage observed by electron microscopy, direct immunofluorescence, and H & E, along with reduced level of anti-nRNP/Sm antibodies (P = 0.048).<h4>Conclusion</h4>The prophylactic co-vaccination of IL-10 with D2 in pristane-induced lupus ameliorates the renal damage maybe by acting as prophylactic DNA tolerizing therapy.
format	article
author	Beatriz Teresita Martín-Márquez Minoru Satoh Rogelio Hernández-Pando Erika Aurora Martínez-García Marcelo Heron Petri Flavio Sandoval-García Oscar Pizano-Martinez Trinidad García-Iglesias Fernanda Isadora Corona-Meraz Monica Vázquez-Del Mercado
author_facet	Beatriz Teresita Martín-Márquez Minoru Satoh Rogelio Hernández-Pando Erika Aurora Martínez-García Marcelo Heron Petri Flavio Sandoval-García Oscar Pizano-Martinez Trinidad García-Iglesias Fernanda Isadora Corona-Meraz Monica Vázquez-Del Mercado
author_sort	Beatriz Teresita Martín-Márquez
title	The DNA co-vaccination using Sm antigen and IL-10 as prophylactic experimental therapy ameliorates nephritis in a model of lupus induced by pristane.
title_short	The DNA co-vaccination using Sm antigen and IL-10 as prophylactic experimental therapy ameliorates nephritis in a model of lupus induced by pristane.
title_full	The DNA co-vaccination using Sm antigen and IL-10 as prophylactic experimental therapy ameliorates nephritis in a model of lupus induced by pristane.
title_fullStr	The DNA co-vaccination using Sm antigen and IL-10 as prophylactic experimental therapy ameliorates nephritis in a model of lupus induced by pristane.
title_full_unstemmed	The DNA co-vaccination using Sm antigen and IL-10 as prophylactic experimental therapy ameliorates nephritis in a model of lupus induced by pristane.
title_sort	dna co-vaccination using sm antigen and il-10 as prophylactic experimental therapy ameliorates nephritis in a model of lupus induced by pristane.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/5f69cb4eb2bf4954bb583b30ad84d7bc
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The DNA co-vaccination using Sm antigen and IL-10 as prophylactic experimental therapy ameliorates nephritis in a model of lupus induced by pristane.

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