A Simplified Quantitative Real-Time PCR Assay for Monitoring SARS-CoV-2 Growth in Cell Culture

A Simplified Quantitative Real-Time PCR Assay for Monitoring SARS-CoV-2 Growth in Cell Culture

ABSTRACT Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected millions within just a few months, causing severe respiratory disease and mortality. Assays to monitor SARS-CoV-2 growth in vitro depend on time-consuming and costly RNA extraction steps, hampering progress in basic r...

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Bibliographic Details
Main Authors: Christian Shema Mugisha, Hung R. Vuong, Maritza Puray-Chavez, Adam L. Bailey, Julie M. Fox, Rita E. Chen, Alex W. Wessel, Jason M. Scott, Houda H. Harastani, Adrianus C. M. Boon, Haina Shin, Sebla B. Kutluay
Format: article
Language:EN
Published: American Society for Microbiology 2020
Subjects:
SARS-CoV-2
antivirals
assay development
viral entry
virus replication
Microbiology
QR1-502
Online Access:https://doaj.org/article/5fa8bcf9f0bd472e90df2dc12e6e4f5e
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https://doaj.org/article/5fa8bcf9f0bd472e90df2dc12e6e4f5e

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