In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting

CRISPR-Cas9-based gene editing involves double-strand breaks at target sequences, which are often repaired by mutagenic non-homologous end-joining. Here the authors use Cas9 nickases to generate coordinated single-strand breaks in donor and target DNA for precise homology-directed gene editing.

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Autores principales: Xiaoyu Chen, Josephine M. Janssen, Jin Liu, Ignazio Maggio, Anke E. J. ‘t Jong, Harald M.M. Mikkers, Manuel A. F. V. Gonçalves
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/60102009427545b88a37146eedf8137c
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